Development and Application of Molecularly Imprinted Polymers for the Selective Extraction of Chlordecone from Bovine Serum

Abstract

The widespread use of chlordecone (CLD), an organochlorine pesticide, until the 1990s to protect banana crops in the French West Indies led to significant pollution of water and soil and, subsequently, of bovine intended for human consumption. Carcasses are submitted to official controls based on perirenal fat CLD determination. In order to allow for pre-slaughter controls, a selective analytical method based on a molecularly imprinted polymer (MIP) associated to the LC/MS-MS method was developed to determine the level of CLD in bovine serum that can be collected before slaughter. Different synthesis conditions were therefore assayed by varying the nature of the monomer and of the porogen, and the most promising MIP in terms of selective retention for CLD (extraction recovery close to 100%) was completely characterized by solid-phase extraction (repeatability of the extraction procedure, of the synthesis, and of the cartridge filling) in pure medium. The capacity of the MIP was determined at 0.13 µmol g−1 of MIP. After application of a spiked bovine serum sample on the MIP, the selective retention was maintained (87 and 21%, respectively, on the MIP and on the corresponding non-imprinted polymer). Moreover, extraction on the MIP led to a cleaner extract compared to those issued from a conventional C18 sorbent.

Hemicryptophane: A Viable Supramolecular Approach to Improve Chlordecone Removal from Water

“…The same order of magnitude was obtained in the previously published study (up to 70 %). [30] Iterative series of hemicryptophane addition and filtration steps were performed. (Figure 3a).…”

“…[26][27] Other research labora-tories have also incorporated cyclodextrins within polymers, nanoporous carbons or molecular printed polymers, to improve retention properties toward various water pollutants with great recovery efficiency. [18,[27][28][29][30] In our case, we considered the use of hemicryptophane supramolecular hosts made of a cyclotriveratrylene (CTV) unit linked to a C 3 -symmetrical plaform. [31] Unlike cyclodextrins, these hosts have the property of being easily modulable in shape and size to specifically recognize well-defined guests.…”

Molecularly Imprinted Polymers: Selective Extraction Materials for Sample Preparation

“…This statement is nicely demonstrated in the paper by Bosman el al. [9], published in this Special Issue. Bosman et al examine the development and application of MIPs for the selective extraction of chlordecone in bovine serum.…”

Molecularly Imprinted Polymer-Based Adsorbents for the Selective Removal of Pharmaceuticals from Wastewater: Adsorption Kinetics, Isotherms, and Thermodynamics Studies

“…MIPs have been widely employed as effective sorbents in traditional solid-phase extraction, also known as molecularly imprinted solid-phase extraction (MISPE) for the extraction of a wide variety of chemicals from water samples . Currently, this is by far the most advanced method for using MIPs to prepare samples. , Following Sellergren and colleagues ground-breaking work in 1994 for the extraction of pentamidine from human urine samples, molecularly imprinted solid-phase extraction (MISPE) has attracted significant interest for the detection of several pharmaceuticals in biological matrices. , Consequently, solid-phase extraction (SPE), which uses stationary phases like nonpolar polymer or C18-bonded silica, is the most used technique for the extraction and cleanup of solutes of interest . The term “sample cleanup” describes the enrichment or extraction of specified analytes from simple or complicated matrices .…”

“…28 Currently, this is by far the most advanced method for using MIPs to prepare samples. 29,30 Following Sellergren and colleagues ground-breaking work in 1994 31 for the extraction of pentamidine from human urine samples, molecularly imprinted solid-phase extraction (MISPE) has attracted significant interest for the detection of several pharmaceuticals in biological matrices. 30,32 Consequently, solid-phase extraction (SPE), which uses stationary phases like nonpolar polymer or C18-bonded silica, is the most used technique for the extraction and cleanup of solutes of interest.…”