Ultra technically-simple and sensitive detection for Salmonella Enteritidis by immunochromatographic assay based on gold growth

Bu, Tong; Huang, Qiong; Yan, Litao; Huang, Lunjie; Zhang, Mengyue; Yang, Qingfeng; Yang, Baowei; Wang, Jianlong; Zhang, Daohong

doi:10.1016/j.foodcont.2017.08.036

Cited by 90 publications

(45 citation statements)

References 37 publications

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“…This allowed for in situ reduction of Au 3+ ions on the surface of primary AuNPs to increase their size, resulting in enhanced signal intensity. Based on this strategy, Bu et al also established an LFA strip to detect Salmonella enteritidis within 20 min by visual observation [ 42 ]. In this assay, a traditional LFA strip (10 min) was used, and then further dipped into an enhancer solution for another 10 min to boost signal intensity.…”

Section: Noble Metal Nanoparticle-mediated Colorimetric Pathogen Detementioning

confidence: 99%

Nanomaterial-mediated paper-based biosensors for colorimetric pathogen detection

Nguyen

Kim

2020

TrAC Trends in Analytical Chemistry

139

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The pervasive spread of infectious diseases and pandemics, such as the 2019 coronavirus disease (COVID-19), are becoming increasingly serious and urgent threats to human health. Preventing the spread of such diseases prioritizes the development of sensing devices that can rapidly, selectively, and reliably detect pathogens at minimal cost. Paper-based analytical devices (PADs) are promising tools that satisfy those criteria. Numerous paper-based biosensors have been established that rival conventional pathogen detection methods. Among them, colorimetric strategies are promising since results can be interpreted by eye, and are simple to operate, which is advantageous for point-of-care testing (POCT). Particularly, the application of nanomaterials on paper-based biosensors has become important as these materials are capable of converting signals from pathogens through unique mechanisms to yield an amplified colorimetric readout. To highlight the research progress on using nanomaterials in colorimetric paper-based biosensor for pathogen detection, we discuss the sensing mechanisms of how they work, structural and analytical characteristics of the devices, and representative recent applications. Current challenges and future directions of using PADs and nanomaterial-mediated strategies are also discussed.

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Section: Noble Metal Nanoparticle-mediated Colorimetric Pathogen Detementioning

confidence: 99%

Nanomaterial-mediated paper-based biosensors for colorimetric pathogen detection

Nguyen

Kim

2020

TrAC Trends in Analytical Chemistry

139

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“…In addition to ELISA, there is another powerful immunodetection technique called lateral flow immunoassay (LFIA), which is a point‐of‐care bioassay performing on paper‐based device (Han et al., ). Typically, a LFIA strip consists of a sample pad for sampling liquid samples, a conjugation pad loaded with dark‐colored NPs labeled with detection antibodies (Ab‐NPs) (Bu et al., ; Wang, Yao et al., ; Yao et al., ), a test pad composed of test line (T‐line) and control line (C line), and an absorbent pad (panel [a] of Figure B). In details, the target analytes are first dropped onto the sample pad (Figure B, ai), and flow with the sample liquid by the capillary force to the conjugation pad, where the analyte‐Ab‐NP complexes are formed through binding of target analytes to the Ab‐NPs (Figure B, aii).…”

Section: Principles Of Detection Using Enzyme‐mimetic Nanomaterialsmentioning

confidence: 99%

“…The visual signal of LFIA is mainly derived from the gathering effect of intrinsically colored NPs, such as AuNPs (Bu et al., ), which will fail if the concentration of NPs is too low to be visible. Therefore, probe materials are of significance to enhance the detection efficiency and sensitivity of LFIA (Bu, Huang, Sung et al., , Bu, Wang et al., ; Dou et al., ).…”

Section: Principles Of Detection Using Enzyme‐mimetic Nanomaterialsmentioning

confidence: 99%

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Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Huang

Sun

et al. 2019

Comp Rev Food Sci Food Safe

Self Cite

130

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The public concerns about agrifood safety call for innovative and reformative analytical techniques to meet the inspection requirements of high sensitivity, specificity, and reproducibility. Enzyme‐mimetic nanomaterials or nanozymes, which combine enzyme‐like properties with nanoscale features, emerge as an excellent tool for quality and safety detection in the agrifood sector, due to not only their robust capacity in detection but also their attraction in future‐oriented exploitations. However, in‐depth understanding about the fundamental principles of nanozymes for food quality and safety detection remains limited, which makes their applications largely empirical. This review provides a comprehensive overview of the principles, designs, and applications of nanozyme‐based detection technique in the agrifood industry. The discussion mainly involves three mimicking types, that is, peroxidase, oxidase, and catalase‐like nanozymes, capable of detecting major agrifood analytes. The current principles and strategies are classified and then discussed in details through discriminating the roles of nanozymes in diverse detection platforms. Thereafter, recent applications of nanozymes in detecting various endogenous ingredients and exogenous contaminants in foods are reviewed, and the outlook of profound developments are explained. Evidenced by the increasing publications, nanozyme‐based detection techniques are narrowing the gap to practical‐oriented food analytical methods, while some challenges in optimization of nanozymes, diversification of recognition‐to‐signal manners, and sustainability of methodology need to conquer in the future.

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“…The growth of the size of gold nanoparticles with the help of the catalyzed reaction of their surface between HAuCl 4 and NH 2 OH was examined by Bu et al [131] as a means of amplification for LFIA. The layered build-up of gold nanoparticles was described by Li et al [132].…”

Section: Proper Response For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

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Ultra technically-simple and sensitive detection for Salmonella Enteritidis by immunochromatographic assay based on gold growth

Cited by 90 publications

References 37 publications

Nanomaterial-mediated paper-based biosensors for colorimetric pathogen detection

Nanomaterial-mediated paper-based biosensors for colorimetric pathogen detection

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

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