Ultra-Performance Liquid Chromatography - An Updated Review

Bhattacharya, Kunal; Chanu, Nongmaithem Randhoni; Bhattacharjee, Atanu; Sahariah, Bhargab Jyoti; Devi, Chanam Melody; Bordoloi, Ripunjoy

doi:10.52711/0974-360x.2022.00987

RJPT

2022

DOI: 10.52711/0974-360x.2022.00987

|View full text |Cite

Ultra-Performance Liquid Chromatography - An Updated Review

Kunal Bhattacharya¹,

Nongmaithem Randhoni Chanu

Atanu Bhattacharjee

et al.

Abstract: Ultra-performance liquid chromatography (UPLC) has an advantage over conventional High-performance liquid chromatography (HPLC) as UPLC offers substantial resolution, speed, and sensitivity during analysis. This advanced chromatographic technique uses sub-2μm particles for the stationary phase. As a result, it saves time and reduces solvent consumption, which allows it to take less run time and makes it highly efficient.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Determination and identification of polyphenols in wine using mass spectrometry techniques

Temerdashev,

Atapattu,

Pamunuwa

2024

Journal of Chromatography Open

View full text Add to dashboard Cite

Determination and identification of polyphenols in wine using mass spectrometry techniques

Temerdashev,

Atapattu,

Pamunuwa

2024

Journal of Chromatography Open

View full text Add to dashboard Cite

Development and Validation of an LC/MS Compatible, Stability indicating RP-UPLC Method for Faropenem

Mathew,

Metri,

Durga

et al. 2024

RJPT

View full text Add to dashboard Cite

In this manuscript an ultrafast, stability-indicating RP-UPLC method with Waters Acquity UPLC having 2996 PDA detection was developed and validated for the quantification of faropenem in the bulk drug and its tablet formulation. The analysis was performed using End version C18 column powered with Software Empower 2 and a mobile phase of 10mM Ammonium formate buffer (pH=3.5): ACN in the ratio of 65: 35(v/v) in isocratic mode at a flow rate of 0.25mL/min at 313nm as the detection wavelength. The linearity range is 5–50μg/mL, and the linear regression analysis of the calibration plot showed a good linear relationship with R2 = 0.9999. The LOD and LOQ were 213 and 638ng/mL, respectively. The stress conditions include acid, alkali, hydrogen peroxide and UV light. The drug peak was well resolved from the degradation products’ peaks, and thereby, the proposed method can be used for the analysis of stability samples.

show abstract

A Review on UHPLC Method

R Patil,

Prakash Patil,

A Patil

2024

RJPPD

View full text Add to dashboard Cite

Ultra-high-pressure liquid chromatography (UHPLC) has emerged as the contemporary HPLC platform of choice in recent years. Because UHPLC requires less time for column equilibration and analysis, it is the perfect tool for developing methods quickly. The present state, advantages, and drawbacks of UHPLC in technique development are critically reviewed in this paper. To explain current developments and best practices, we employ case studies. Converting current HPLC methods to speedier analysis, quick screening of columns and mobile phases, and automated process optimisation are a few examples. Although the development of reversed-phase techniques for small-molecule pharmaceutical assay and impurity analysis is our primary emphasis, our findings and insights are applicable to other sample types and applications as well. When utilised with short, small-particle columns, UHPLC's greater pressure limitations not only produce quicker analysis but also make it possible to use longer columns more effectively for improved routine analysis of complicated materials.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Ultra-Performance Liquid Chromatography - An Updated Review

Cited by 3 publications

References 21 publications

Determination and identification of polyphenols in wine using mass spectrometry techniques

Determination and identification of polyphenols in wine using mass spectrometry techniques

Development and Validation of an LC/MS Compatible, Stability indicating RP-UPLC Method for Faropenem

A Review on UHPLC Method

Contact Info

Product

Resources

About