A Review on UHPLC Method

Abstract

Ultra-high-pressure liquid chromatography (UHPLC) has emerged as the contemporary HPLC platform of choice in recent years. Because UHPLC requires less time for column equilibration and analysis, it is the perfect tool for developing methods quickly. The present state, advantages, and drawbacks of UHPLC in technique development are critically reviewed in this paper. To explain current developments and best practices, we employ case studies. Converting current HPLC methods to speedier analysis, quick screening of columns and mobile phases, and automated process optimisation are a few examples. Although the development of reversed-phase techniques for small-molecule pharmaceutical assay and impurity analysis is our primary emphasis, our findings and insights are applicable to other sample types and applications as well. When utilised with short, small-particle columns, UHPLC's greater pressure limitations not only produce quicker analysis but also make it possible to use longer columns more effectively for improved routine analysis of complicated materials.