Ultra high performance liquid chromatography versus high performance liquid chromatography: Stationary phase selectivity for generic carotenoid screening

Bijttebier, Sebastiaan; Dhondt, Elisabeth; Noten, Bart; Hermans, Nina; Apers, Sandra; Voorspoels, Stefan

doi:10.1016/j.chroma.2014.01.042

Cited by 47 publications

(29 citation statements)

References 38 publications

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“…Therefore, the extraction methods mean the application of similar protocols applied for the extraction of free carotenoids, but the oily-dense nature of some matrices containing carotenoid esters requires clean-up steps. Different approaches have been set up, including the utilization of open column chromatography on silica gel [17] or magnesium oxide [18], enzymatic hydrolysis [19], solid-phase extraction [20], or even the use of semi-preparative isolation on C18 column and subsequent identification [21].…”

Section: Extraction Of Carotenoids and Sample Preparation For Analysismentioning

confidence: 99%

Recent Developments in the Analysis of Carotenoids by Mass Spectrometry

Pérez‐Gálvez¹,

Roca²

2018

Progress in Carotenoid Research

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Mass spectrometry has become an indispensable tool for the analysis of carotenoids in photosynthetic tissues, foods, and biological materials from different sources to accurately establish their pigment profile, to provide evidences to distinguish the different structural arrangements, and to obtain biological meaning from metabolic processes where carotenoids participate during the development of their natural functions and activities. The recent progresses in the hyphenated HPLC systems with hybrid mass spectrometers, which enhance the acquisition of independent and complementary physicochemical properties for the identification of carotenoids, are detailed in this chapter. A reasonable guide for the implementation of post-processing routines, assisted by modern software tools, and the key issues for the analysis of the characteristic product ions are also defined in this contribution to help the readers in the understanding of the potential capabilities of mass spectrometry in the field of carotenoid pigments.

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Section: Extraction Of Carotenoids and Sample Preparation For Analysismentioning

confidence: 99%

Recent Developments in the Analysis of Carotenoids by Mass Spectrometry

Pérez‐Gálvez¹,

Roca²

2018

Progress in Carotenoid Research

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“…The separation of carotenoids is generally carried out using C 18 or C 30 stationary phases. The more hydrophobic C 30 phase has been demonstrated to separate carotenoids more efficiently, especially the Z / E isomers of lutein and zeaxanthin . Carotenoids in foods occur as complex mixtures and are highly sensitive to oxidation upon exposure to light, high temperature, oxygen, acid, certain enzymes, etc.…”

Section: Introductionmentioning

confidence: 99%

A fast isocratic liquid chromatography method for the quantification of xanthophylls and their stereoisomers

et al. 2015

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A fast isocratic liquid chromatography method was developed for the simultaneous quantification of eight xanthophylls (13-Z-lutein, 13'-Z-lutein, 13-Z-zeaxanthin, all-E-lutein, all-E-zeaxanthin, all-E-canthaxanthin, all-E-β-apo-8'-carotenoic acid ethyl ester and all-E-β-apo-8'-carotenal) within 12 min, compared to 90 min by the conventional high-performance liquid chromatography method. The separation was achieved on a YMC C30 reversed-phase column (100 mm x 2.0 mm; 3 μm) operated at 20°C using a methanol/tert-butyl methyl ether/water solvent system at a flow rate of 0.8 mL/min. The method was successfully applied to quantify lutein and zeaxanthin stereoisomers in egg yolk, raw and cooked spinach, and a dietary supplement. The method can be used for the rapid analysis of xanthophyll isomers in different food products and for quality control purposes.

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“…Thus, UPLC offers several advantages over conventional HPLC, such as rapid analysis with shorter retention time, narrower peaks giving increased signal-to-noise ratio with high resolution and sensitivity. However, the drawback of UHPLC is currently there is no C 30 stationary phase, which is considered as a 'golden standard' in the chromatographic separation of carotenoids (Bijttebier et al, 2014). Generally, closely related carotenoids and their metabolites co-elute in various analytical methods.…”

Section: Metabolism Of Oxygenated Carotenoidsmentioning

confidence: 99%

“…This facilitates identification of unknown compounds and helps in the elucidation of fragmentation pattern. Bijttebier et al (2014) reported for the first time using orbitrap technology for the elucidation of fragmentation pathways of carotenoids. More recently, Van Meulebroek, Vanden Bussche, Steppe, and Vanhaecke (2014) developed a full-scan high-resolution metabolomic screening for carotenoids in tomato fruit by using orbitrap-mass spectrometry.…”

Section: Metabolism Of Oxygenated Carotenoidsmentioning

confidence: 99%

“…Previously, chromatographic separation of carotenoids was based on HPLC analysis using C 18 and C 30 columns. In general, C 30 and C 18 stationary phases are extremely employed for the separation of geometrical isomers (Bijttebier et al, 2014;Liu, Lee, Garofalo, Jenkins, & EliSohemy, 2011). Advancement in Liquid chromatography for separation of carotenoids and metabolites using ultra high-performance liquid chromatography (UPLC) are of current interest.…”

Section: Metabolism Of Oxygenated Carotenoidsmentioning

confidence: 99%

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Metabolomics of carotenoids: The challenges and prospects – A review

Arathi

Sowmya

Vijay

et al. 2015

Trends in Food Science & Technology

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Considerable progress in carotenoids research has been made to understand the carotenoid metabolism in animals including human. Epidemiological and clinical studies have correlated with dietary intake of carotenoids on reduction of vitamin A deficiency, age-related macular degeneration, cancer and cardiovascular diseases. Recent findings demonstrate the existence of carotenoid metabolites in vivo and their efficacy have made greater insight on prospecting carotenoid metabolites. Owing to their biological activity, exploration of analytical methods for the characterization of carotenoid metabolites is considered to be important before addressing the stability and bioactivity. Although few studies are available on carotenoid metabolites, their structural characterization in biological samples require a substantial refining of analytical protocols like isolation, purification, prerequisite of equipment parameters and robustness in hyphenated techniques.Recently, researchers have focused on biotransformation of carotenoids and made an attempt to screen their metabolites by high-throughput analytical strategies. However, till date there is no detailed analytical techniques available to fingerprint carotenoid metabolites, due to interference with complex biological matrices. This review highlights the carotenoid metabolism, possible bioconversion and available bio-analytical techniques to characterize metabolites in vivo. Further, advancement in sensitivity, mode of ionization and fragmentation patterns of metabolites were also discussed. The identification of carotenoid metabolites in system specific will have further insight in the emerging field of nutritional metabolomics.

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Ultra high performance liquid chromatography versus high performance liquid chromatography: Stationary phase selectivity for generic carotenoid screening

Cited by 47 publications

References 38 publications

Recent Developments in the Analysis of Carotenoids by Mass Spectrometry

Recent Developments in the Analysis of Carotenoids by Mass Spectrometry

A fast isocratic liquid chromatography method for the quantification of xanthophylls and their stereoisomers

Metabolomics of carotenoids: The challenges and prospects – A review

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