Ultra high performance liquid chromatography coupled to tandem mass spectrometry determination of lipid peroxidation biomarkers in newborn serum samples

Prenatal exposure to particulate matter and ozone: Bulky DNA adducts, plasma isoprostanes, allele risk variants, and neonate susceptibility in the Mexico City Metropolitan Area

“…The levels of 8-iso-PGF 2α were determined in both maternal and cord blood plasma. Maternal and cord blood isoprostanes correlated significantly (ρ = 0.265, 95% CI [−0.039, 0.524], P = 0.039), although cord blood levels were significantly higher (P < 0.0001) (Table I) as previously reported Chafer-Pericas et al, 2015). In addition, we observed a significant association between maternal 8-iso-PGF 2α and ozone levels only during the last month of pregnancy (ρ = 0.322, 95% CI [0.067, 0.538], P = 0.0060), but they were not associated A B Fig.…”

DHA reduces oxidative stress following hypoxia-ischemia in newborn piglets: a study of lipid peroxidation products in urine and plasma

“…The technique is used in pharmacokinetics and for structural elucidation giving information about fragmentation patterns. The details on methodology are found in paper I and II and in the publications of Chafer-Pericas et.al 179 and Casetta et.al 180 .…”

Preliminary case control study to establish the correlation between novel peroxidation biomarkers in cord serum and the severity of hypoxic ischemic encephalopathy

“…Significant elevation of isoprostanes has been detected in neurodegenerative diseases [35], and HIE [15]. Our group has developed and validated highly reliable methods based on ultra-performance liquid chromatography coupled to tandem mass spectrometry to determine prostaglandin-like and derivatives in plasma, serum, and urine of newborn infants [19,30]. We wanted to establish a correlation between severe metabolic acidosis and increased prostanoidderivatives as a first step to identify possible early and reliable markers of brain damage.…”

“…The conditions employed were established in a recently published work [30]. Briefly, negative electrospray ionization (ESI À ), capillary voltage 3.5 kV, source temperature 120°C, desolvation temperature 300°C, nitrogen cone and desolvation gas flows were 25 and 680 Lh…”

“…The sample treatment was optimized and assayed as described before [30]. Briefly, serum samples were thawed on ice, and 100 mL of sample was added to 3 mL of internal standard (IS) solution PGF 2α -D4 20 mmol L À 1 and to 100 mL of potassium hydroxide (15%, w/v) incubating at 40°C for 30 min.…”