Ultra fast in vivo microwave irradiation for enhanced metabolic stability of brain biopsy samples during HRMAS NMR analysis

Détour, Julien; Elbayed, Karim; Piotto, Martial; Moussallieh, François‐Marie; Nehlig, Astrid; Namer, Izzie Jacques

doi:10.1016/j.jneumeth.2011.07.014

Cited by 16 publications

(40 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This provides a metabolite signature comparable to 1 H MRS metabolite stability measurements (de Graaf et al 2009) and validated by high resolution magic angle spinning NMR (Detour et al 2011). Importantly, the targeted metabolite profiling assays performed show the role of tissue preservation dependent on processing techniques.…”

Section: Discussionmentioning

confidence: 74%

Combinatorial assessments of brain tissue metabolomics and histopathology in rodent models of human immunodeficiency virus infection

Epstein

Narayanasamy

Dash

et al. 2013

J Neuroimmune Pharmacol

View full text Add to dashboard Cite

Metabolites are biomarkers for a broad range of central nervous system disorders serving as molecular drivers and byproducts of disease pathobiology. However, despite their importance, routine measures of brain tissue metabolomics are not readily available based on the requirements of rapid tissue preservation. They require preservation by microwave irradiation, rapid freezing or other methods designed to reduce post mortem metabolism. Our research on human immunodeficiency virus type one (HIV-1) infection has highlighted immediate needs to better link histology to neural metabolites. To this end, we investigated such needs in well-studied rodent models. First, the dynamics of brain metabolism during ex vivo tissue preparation was shown by proton magnetic resonance spectroscopy in normal mice. Second, tissue preservation methodologies were assessed using liquid chromatography tandem mass spectrometry and immunohistology to measure metabolites and neural antigens. Third, these methods were applied to two animal models. In the first, immunodeficient mice reconstituted with human peripheral blood lymphocytes then acutely infected with HIV-1. In the second, NOD scid IL2 receptor gamma chain knockout mice were humanized with CD34+ human hematopoietic stem cells and chronically infected with HIV-1. Replicate infected animals were treated with nanoformulated antiretroviral therapy (nanoART). Results from chronic infection showed that microgliosis was associated with increased myoinostitol, choline, phosphocholine concentrations and with decreased creatine concentrations. These changes were partially reversed with nanoART. Metabolite responses were contingent on the animal model. Taken together, these studies integrate brain metabolomics with histopathology towards uncovering putative biomarkers for neuroAIDS.

show abstract

Section: Discussionmentioning

confidence: 74%

Combinatorial assessments of brain tissue metabolomics and histopathology in rodent models of human immunodeficiency virus infection

Epstein

Narayanasamy

Dash

et al. 2013

J Neuroimmune Pharmacol

View full text Add to dashboard Cite

show abstract

“…lung, muscle, kidney, liver and pancreas), even the short delay between brain isolation and fixation results in metabolic alterations under hypoxemic conditions (Griffin and Salek, 2007). Additionally, metabolites are degraded at different rates, which further complicate analysis (de Graaf, et al, 2009; Detour, et al, 2011). In particular, glucose and energy-linked phosphates are prone to degradation within seconds of mammalian death (Lowry, et al, 1964).…”

Section: Resultsmentioning

confidence: 99%

“…Fixation methods, such as funnel freezing or the application of microwaves on an intact live brain coupled to 1 H MRS validation, as used in this report, insure the efficient quenching of metabolism (Detour, et al, 2011). When metabolism quenching is performed correctly, the advantage of brain tissue over biofluid profiling is significant as limited passage of metabolites by BBB, and differences between the brain parenchyma and CSF, present major pitfalls for the use of biomarkers from biofluid analysis.…”

Section: Resultsmentioning

confidence: 99%

Brain Region Mapping Using Global Metabolomics

Ivanišević¹,

Epstein²,

Kurczy³

et al. 2014

Chemistry & Biology

View full text Add to dashboard Cite

SUMMARY Historically, studies of brain metabolism have been based on targeted analyses of a limited number of metabolites. Here we present a novel untargeted mass spectrometry-based metabolomics approach that has successfully uncovered differences in broad array of metabolites across anatomical regions of the mouse brain. The NSG immunodeficient mouse model was chosen because of its ability to undergo humanization leading to numerous applications in oncology and infectious disease research. Metabolic phenotyping by hydrophilic interaction liquid chromatography and nanostructure imaging mass spectrometry revealed unique water-soluble and lipid metabolite patterns between brain regions. Neurochemical differences in metabolic phenotypes were mainly defined by various phospholipids and several intriguing metabolites including carnosine, cholesterol sulfate, lipoamino acids, uric and sialic acid whose physiological roles in brain metabolism are poorly understood. This study lays important groundwork by defining regional homeostasis for the normal mouse brain to give context to the reaction to pathological events.

show abstract

“…Heating tissue samples rapidly to 80–90°C for 1–2 s with pulsed microwaves inactivates enzyme activity within the tissue specimens and “freezes” the tissue's metabolic state for HRMAS analysis. No significant change was seen either in the metabolic profile from HRMAS measurement of samples at 37°C or in tissue histology following microwave radiation . The impacts of sample ischemia, prolonged spinning times, and freeze‐thawing of samples have also been studied.…”

Section: Improving Methodologiesmentioning

confidence: 99%

Applications of high‐resolution magic angle spinning MRS in biomedical studies I—cell line and animal models

et al. 2017

View full text Add to dashboard Cite

Summary High-resolution magic angle spinning (HRMAS) magnetic resonance spectroscopy (MRS) allows for direct measurements of non-liquid tissue and cell specimens to present valuable insights of the cellular metabolisms of physiological and pathological processes. HRMAS produces high resolution spectra comparable to those obtained from solutions of specimen extracts but without complex metabolite extraction processes, and preserves the tissue cellular structure suitable for pathological examinations following spectroscopic analysis. The technique has been applied in a wide variety of biomedical and biochemical studies and become one of the major platforms of metabolomic studies. By quantifying single metabolites, metabolite ratios or metabolic profiles in their entirety, HRMAS presents promising possibilities for diagnosis and prediction of clinical outcomes for various diseases, as well as deciphering of metabolic changes resulting from drug therapies or xenobiotic interactions. In this review, we evaluate HRMAS MRS results on animal models and cell lines reported in literature, and present the diverse applications of the method for the understanding of pathological processes, the effectiveness of therapies, the developments of disease animal models, and the new progresses in the HRMAS methodology.

show abstract

Ultra fast in vivo microwave irradiation for enhanced metabolic stability of brain biopsy samples during HRMAS NMR analysis

Cited by 16 publications

References 28 publications

Combinatorial assessments of brain tissue metabolomics and histopathology in rodent models of human immunodeficiency virus infection

Combinatorial assessments of brain tissue metabolomics and histopathology in rodent models of human immunodeficiency virus infection

Brain Region Mapping Using Global Metabolomics

Applications of high‐resolution magic angle spinning MRS in biomedical studies I—cell line and animal models

Contact Info

Product

Resources

About