Ultra‐Early Diagnosis of Acute Myocardial Infarction in Rats Using Ultrasound Imaging of Hollow Double‐Layer Silica Nanospheres

Guo, Mengzhe; Du, Wencheng; Lyu, Nan; Chen, Xi; Du, Yan; Wang, Haibo; Yang, Dongzhi; Wu, Shihua; Liang, Jun; Pan, Yuanjiang; Tang, Daoquan

doi:10.1002/adhm.201901155

Cited by 8 publications

(7 citation statements)

References 44 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While both controls (Figures 6 and S8) injected in the vascular phantom channels show nearly no signal in the ultrasound, metal-doped NGQDs exhibit substantial scattering and, thus, provide ultrasound contrast as they move through the channels (Figure 6). Ultrasound intensity from the metal-doped NGQDs in the vascular phantom is comparable to hollow silicon nanoparticles at 1 mg/mL 54 and oxidized single-wall carbon nanotubes at 1 mg/mL 48 tested in vitro. Image analyses of metal-doped and non-doped NGQDs yield statistically significant difference (P < 0.05), indicating that all metal-doped and non-doped NGQDs are echogenic as compared to the control water, although metal-doped NGQDs demonstrate around 10 times higher intensity.…”

Section: Resultsmentioning

confidence: 83%

Dual-Mode Fluorescence/Ultrasound Imaging with Biocompatible Metal-Doped Graphene Quantum Dots

Valimukhametova

Zub

Lee

et al. 2022

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Sonography offers many advantages over standard methods of diagnostic imaging due to its non-invasiveness, substantial tissue penetration depth, and low cost. The benefits of ultrasound imaging call for the development of ultrasound-trackable drug delivery vehicles that can address a variety of therapeutic targets. One disadvantage of the technique is the lack of highprecision imaging, which can be circumvented by complementing ultrasound contrast agents with visible and, especially, near-infrared (NIR) fluorophores. In this work, we, for the first time, develop a variety of lightly metal-doped (iron oxide, silver, thulium, neodymium, cerium oxide, cerium chloride, and molybdenum disulfide) nitrogen-containing graphene quantum dots (NGQDs) that demonstrate high-contrast properties in the ultrasound brightness mode and exhibit visible and/or near-infrared fluorescence imaging capabilities. NGQDs synthesized from glucosamine precursors with only a few percent metal doping do not introduce additional toxicity in vitro, yielding over 80% cell viability up to 2 mg/mL doses. Their small (<50 nm) sizes warrant effective cell internalization, while oxygen-containing surface functional groups decorating their surfaces render NGQDs water soluble and allow for the attachment of therapeutics and targeting agents. Utilizing visible and/or NIR fluorescence, we demonstrate that metal-doped NGQDs experience maximum accumulation within the HEK-293 cells 6−12 h after treatment. The successful 10-fold ultrasound signal enhancement is observed at 0.5−1.6 mg/mL for most metal-doped NGQDs in the vascular phantom, agarose gel, and animal tissue. A combination of non-invasive ultrasound imaging with capabilities of high-precision fluorescence tracking makes these metal-doped NGQDs a viable agent for a variety of theragnostic applications.

show abstract

Section: Resultsmentioning

confidence: 83%

Dual-Mode Fluorescence/Ultrasound Imaging with Biocompatible Metal-Doped Graphene Quantum Dots

Valimukhametova

Zub

Lee

et al. 2022

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…Hollow nanometric silica structures (HNSS) were designed with a size of 385 nm and an internal compartment content made of perfluorinated compounds to yield an ultrasound contrasting effect. Furthermore, they were associated with an antibody targeting hs-cTnI, which is a well-known marker of myocardial damage, thus allowing the detection by ultrasound imaging of acute myocardial infarction (AMI) following intravenous injection of HNSS to rats [33]. HNSS are characterized by two additional advantages.…”

Section: Various Types Of Nanomaterials Used As Theragnostic Ultrasou...mentioning

confidence: 99%

“…On the one hand, their stability can be achieved by an original method relying on the opposite polarities between the internal and external compartments of such structures. On the other hand, the presence of mesopores within silica promotes the attachment of compounds such as targeting agents to HNSS [33].…”

Section: Various Types Of Nanomaterials Used As Theragnostic Ultrasou...mentioning

confidence: 99%

Nanomaterials as Ultrasound Theragnostic Tools for Heart Disease Treatment/Diagnosis

Alphandéry

2022

IJMS

View full text Add to dashboard Cite

A variety of different nanomaterials (NMs) such as microbubbles (MBs), nanobubbles (NBs), nanodroplets (NDs), and silica hollow meso-structures have been tested as ultrasound contrast agents for the detection of heart diseases. The inner part of these NMs is made gaseous to yield an ultrasound contrast, which arises from the difference in acoustic impedance between the interior and exterior of such a structure. Furthermore, to specifically achieve a contrast in the diseased heart region (DHR), NMs can be designed to target this region in essentially three different ways (i.e., passively when NMs are small enough to diffuse through the holes of the vessels supplying the DHR, actively by being associated with a ligand that recognizes a receptor of the DHR, or magnetically by applying a magnetic field orientated in the direction of the DHR on a NM responding to such stimulus). The localization and resolution of ultrasound imaging can be further improved by applying ultrasounds in the DHR, by increasing the ultrasound frequency, or by using harmonic, sub-harmonic, or super-resolution imaging. Local imaging can be achieved with other non-gaseous NMs of metallic composition (i.e., essentially made of Au) by using photoacoustic imaging, thus widening the range of NMs usable for cardiac applications. These contrast agents may also have a therapeutic efficacy by carrying/activating/releasing a heart disease drug, by triggering ultrasound targeted microbubble destruction or enhanced cavitation in the DHR, for example, resulting in thrombolysis or helping to prevent heart transplant rejection.

show abstract

“…Imaging‐guided therapy of cancer is a novel oncological technique that minimizes the invasiveness of surgical intervention. With targeting ability to tumors, MONs‐based nanosystems have been developed to serve as imaging probes for theranostic applications, such as magnetic resonance, fluorescence, [ 125,126 ] ultrasound, [ 127 ] and photoacoustic [ 128 ] imaging. The introduction of functional organic groups to MONs can easily integrate the diagnostic and therapeutic capabilities into a single nanoparticle.…”

Section: Tuning Siliceous Framework By Incorporation Of Organic Speciesmentioning

confidence: 99%

Tuning Nanosiliceous Framework for Enhanced Cancer Theranostic Applications

et al. 2021

Advanced Therapeutics

View full text Add to dashboard Cite

As nanotechnology rapidly advances, siliceous nanomaterials have become increasingly examined for biomedical applications. To further expand their advantageous physicochemical properties and counter intrinsic limitations challenging their progress toward clinical translation, strategies ranging from new fabrication methods to diverse forms of modifications have been proposed. Among them, direct incorporation of foreign species into the siliceous frameworks has emerged. The incorporation of inorganic or organic moieties into an otherwise purely siliceous framework enriches the structure with novel or improved properties. In this review, the progress of inorganic modification is mainly examined on mesoporous silica nanoparticles (MSNs) due to their widely appreciated attributes that garnered them an important position within cancer nanomedicine, while organic modifications are studied through designs of mesoporous organosilica nanoparticles (MONs). General synthesis methods and philosophies behind framework modifications are first discussed in brief. Subsequently, several interesting inorganically doped MSNs and formulations of MONs are studied for their advantages, including the role of catalysis after metal impregnation, improved biodegradability, novel stimuli‐responsiveness, role in bioimaging, and enhancing the outcomes of key treatment strategies. Finally, the advances are summarized, along with proposed prospects for these hybrid materials in their progress toward clinical use.

show abstract

Ultra‐Early Diagnosis of Acute Myocardial Infarction in Rats Using Ultrasound Imaging of Hollow Double‐Layer Silica Nanospheres

Cited by 8 publications

References 44 publications

Dual-Mode Fluorescence/Ultrasound Imaging with Biocompatible Metal-Doped Graphene Quantum Dots

Dual-Mode Fluorescence/Ultrasound Imaging with Biocompatible Metal-Doped Graphene Quantum Dots

Nanomaterials as Ultrasound Theragnostic Tools for Heart Disease Treatment/Diagnosis

Tuning Nanosiliceous Framework for Enhanced Cancer Theranostic Applications

Contact Info

Product

Resources

About