Uji Toksisitas Akut Nanoinsektisida Tembakau (Nicotiana tabacum L.) terhadap Mencit

Abstract

Tobacco (Nicotiana tabacum) is a plant that can be used as a natural insecticide. Tobacco extracts can be made as alternative nanoinsecticides to be used as Aedes aegypti control. This study aims to obtain information on the toxicity of tobacco nanoinsecticides and obtain the LD50 of tobacco nanoinsecticides in mice. The research was conducted at the Center for Disease Vector and Reservoir Research and Development (B2P2VRP) with a pure experimental method. The results showed that the particle size ranged from 26.9; 53.7 and 89nm. The results of the metal content test with an Atomic Absorption Spectrophotometer (AAS) showed the presence of Ag content in the nanoinsecticide solution made. Spectrophotometry measurements showed a peak at a wavelength of 420 nm and functional group bonds were formed. The characteristic SEM photo shows the particle morphology in the form of round-face crystals. The results of the toxicity test using the OECD 423 LD50 method at 2500mg/kg BB showed that tobacco nanoinsecticides were in the slightly toxic category. Administration of tobacco nanoinsecticides causes toxic symptoms in the form of decreased cardiac activity, convulsions, and decreased movement activity, while from the histopathological test results, the number of kidney injuries is in the range of 10 to 25%.

Literature Review: Pharmacological and Toxicological Effects of Plants From the Solanaceae Family

“…The toxicity test of the thick extract of tobacco leaves was carried out on test animals of female DDY strain rats at doses of 300, 2000, and 2500 mg/kgBW given orally, while the nanoinsecticide preparation for tobacco was given a dose of 2500 mg/kgBW orally. Based on research results (Handayani, 2022), rats did not show any toxic effects from the extract at a dose of 300 mg/kgBW, then continued at a dose of 2000 mg/kgBW they began to show side effects from the extract treatment, such as stress, convulsions and one mouse which died, and at a dose of 2500 mg/kgBW, similar toxic effects were shown by rats with stable body weight. Apart from that, the tobacco nanoninsecticide toxicity test was also carried out at a dose of 2500 mg/kgBW, and a similar toxic effect was produced as treatment with a thick extract of tobacco leaves.…”

Antimicrobial Activity of Tobacco Flower Extract (Nicotiana tabacum L.) in Various Solvent

“…Tobacco flowers were washed thoroughly with running water, drained, dried at 45 ºC to obtain dry simplicia. The dried simplicia was pulverized into powder and sieved using a 40 mesh sieve [4] [8].…”

“…Tobacco flower simplicia powder was extracted using maceration method with methanol, ethanol, and N-hexane solvents (1:10) [8]. The extract was filtered using filter paper and concentrated using a rotary evaporator and evaporated in a waterbath to obtain a liquid extract of tobacco flower.…”

PERBANDINGAN MORFOLOGI TELUR Taenia saginata YANG DISIMPAN DENGAN VARIASI SUHU DAN WAKTU PENYIMPANAN

“…Namun, infeksi juga bisa terjadi melalui sayuran yang terkontaminasi oleh telur cacing pita, serta penggunaan pupuk organik yang berasal dari kotoran hewan seperti sapi atau kambing. 16 Pemeriksaan kecacingan dapat dilakukan melalui dua metode, yaitu pemeriksaan langsung dan tidak langsung.…”

“…feses secara langsung dari rectum sapi atau diambil sebelum feses jatuh ke tanah akan sangat beresiko. 16 Sehingga, pengambilan sampel feses yang aman ialah mengambil yang sudah jatuh ke tanah kurang dari 3 jam setelah sapi mengalami defekasi. Struktur feses sapi yang didapatkan ialah agak lembek dan warnanya coklat kehijauan.…”