Uji Penghambatan Minyak Atsiri Kayu Putih dan Kunyit terhadap Patogen Penyebab Penyakit pada Tanaman Murbei Secara In Vitro

Abstract

Mulberry plants (Morus sp.) are one of the non-timber forest products that are used as feedfor silkworms, foodstuffs, or medicines. Plants that are attacked by disease can certainly harmvarious aspects, one of which can reduce the quality and quantity of plants. Vegetable pesticidescan be a solution to prevent the spread of the disease. This study aims to determine the symptomsof diseases that appear in mulberry plants and their spread in mahogany and find out theinhibitory power and concentration of essential oils that work optimally. Observations weremade using eucalyptus essential oil, turmeric essential oil, and a combination of both withconcentrations of 1%, 3%, and 5% on PDA media and GDP media. Based on observations, thetype of fungus found is Rhizoctonia sp.. The results of the observations showed that the optimalessential oil as an inhibitor of pathogenic growth is turmeric essential oil with a concentration of3% and a combined essential oil concentration of 5%. The results of the inoculation showed thatthere was fungal growth activity on mahogany seedlings so that the fungus could attack otherplants such as forestry plants.

Characterization of endophytic bacteria of Mindi (Melia azedarach) and Balsa (Ochroma pyramidale) plants and potential inhibition in plant diseases

“…This inhibition is also evidenced in the inhibition graph (Figure 1), which shows inhibited pathogen colonies on the first day compared to controls. Pathogen inhibition produced significant values on the first day and remained stagnant until the end of the observation [14]. Based on the morphological inhibition test results, it can be seen in Figure 1 that all the isolates obtained inhibited the pathogen from the first day and remained stagnant until the seventh day.…”

The Influence of Patchouli (Pogostemon cablin Benth.) and Turmeric (Curcuma domestica Val.) essential oils on the growth of Rhizoctonia sp. in vitro

“…The solution is then added to sterile water for 10 ml of essential oil. The composition used follows a modified research approach based on Firmansyah and Yuwono [12] and Pratiwi [13] (Table 1 and Table 2…”

“…The essential oil solutions are adjusted according to their types and concentrations and added to the PDA and PDB growth media. For each petri dish, 9.9 ml of liquid PDA is combined with 1.1 ml of the concentration solution, while for each jar, 29.7 ml of PDB is mixed with 0.3 ml of the concentration solution [12]. The essential oil and media mixture is carefully performed using a syringe within a controlled laminar airflow environment.…”

“…It is then weighed to determine its dry weight and the relative inhibition level is calculated accordingly. The formulas for calculating mycelium biomass [14] and relative inhibition [12] are as follows: Relative Inhibition (%) on PDB = B1 -B2 B1 x 100%…”