UJI FITOKIMIA DAN AKTIVITAS ANTIBAKTERI FRAKSI ETIL ASETAT, KLOROFORM DAN PETROLEUM ETER EKSTRAK METANOL ALGA COKLAT Sargassum vulgare DARI PANTAI KAPONG PAMEKASAN MADURA

Khoiriyah, Siti Imama; Hanapi, Ahmad; Fasya, Ahmad Ghanaim

doi:10.18860/al.v0i1.2914

Cited by 9 publications

(15 citation statements)

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“…The maceration used the extraction method because it was simply done by soaking, without heating, thus avoiding damage to the compound content (Dean, 2009). The solvent used in the extraction process was ethanol 96%, which was selected based on the safety and ease level when evaporating (Khoiriyah, 2014). Nevertheless, the maceration method's disadvantage is that it requires a lot of solvents and no less than perfect extraction, so stirring is essential to optimize the method.…”

Section: Resultsmentioning

confidence: 99%

Gastroprotective effect of Canna edulis Ker. ethanolic extract in piroxicam-induced rats

Tilarso¹,

Bachri²,

Widyaningsih³

2021

Pharmaciana

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Medications derived from plants and pure natural ingredients have far lower side effects and risks than synthetic drugs. One side effect of piroxicam is irritation of the digestive tract. One of the therapeutic preventions to minimize peptic ulcers was utilizing Canna edulis Ker. This study aims to prove that ethanolic extract of Canna edulis Ker. can be used as an alternative to prevent piroxicaminduced peptic ulcers based on ulcer index parameters and the protection ratio. The rats were divided randomly into 6 groups consisting of 5 rats. The normal group was given food and water. The negative control group was given 0.5% CMC-Na. The extract groups were given various doses of ethanolic extract of Canna edulis Ker. (50, 100, and 200 mg/kg BW). The positive control was given sucralfate at 360 mg/kg BW dose. Rats were treated orally for 14 days. One hour after the treatment on the 14th day, all groups except group I were orally administered with piroxicam dissolved in 0.5% CMC-Na at 1.8 mg/kg BW dose. Twenty-four hours later, animals were sacrificed, dissected, and their stomach organs were removed to analyze its number of ulcers. Ulcer observation was formed by giving a score and protection ratio. The mean ulcer index value was 0.33±0.58 for 50 mg/kg BW and 100 mg/kg BW ethanol extract treatment groups, while the 200 mg/kg BW group showed 0.67±0.58. The protection ratio was 83.33±28.87 in 50 mg/kg BW and 100 mg/kg BW treatment groups, while 66.67±28.87 was shown in the 200mg/kg BW group. Canna edulis Ker. ethanolic extract has the gastroprotective ability by decreasing the index of gastric ulcers and increasing the protection ratio to the stomach of piroxicam-induced rats.

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Section: Resultsmentioning

confidence: 99%

Gastroprotective effect of Canna edulis Ker. ethanolic extract in piroxicam-induced rats

Tilarso¹,

Bachri²,

Widyaningsih³

2021

Pharmaciana

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“…Menurut Khoiriyah dkk. [39], kadar air yang rendah dapat mempermudah proses penarikan zat aktif dalam sampel karena pelarut mudah menembus dinding sel sampel tanpa adanya gangguan dari molekul air. Hasil penentuan kadar air pada sampel kering H. verticillata sebesar 6,5%.…”

Section: Penentuan Kadar Air Secara Termogravimetriunclassified

Aktivitas Antioksidan Isolat Steroid Hasil Kromatografi Lapis Tipis dari Fraksi n-Heksana Hydrilla verticillata

Fasya

Purwantoro

Ulya

et al. 2020

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Hydrilla verticillata is one of Allah SWT creations that potential as an antioxidant. This study’s objectives were to determine antioxidant activity and identify steroid isolates of n-hexane fraction of H. verticillata. The steroid compounds were extracted using methanol solvent. The methanol extract was hydrolyzed with HCl 2 N and partitioned using n-hexane. The n-hexane fraction was tested phytochemically and separated using thin-layer chromatography (TLC). The steroids compounds were identified using UV-Vis spectrophotometer, FTIR, and LC-MS/MS. The result showed that maceration extraction produced 5.14% yield, whereas n-hexane fraction produce 47.95% yield. Steroid separation through analytical TLC revealed that n-hexane: ethyl acetate (4:1) as the best eluant with 12 spots, while separation using preparative TLC yielded 19 spots. Steroid isolate from TLC preparative has antioxidant activity with EC50 of 5.109 ppm. Identification of steroid compounds using UV-Vis produced maximum wavelengths at 203.9 and 276 nm, while using FTIR indicated the presence of O-H group, geminal dimethyl, C=O, C=C, secondary C-OH, and =C-H (alkene) which might contain steroid compounds. The result of LC-MS/MS showed the presence of β-sitosterol. Hydrilla verticillata merupakan salah satu ciptaan Allah SWT yang memiliki potensi sebagai antioksidan. Penelitian ini bertujuan mengetahui aktivitas antioksidan dan mengidentifikasi senyawa steroid pada isolat hasil pemisahan fraksi n-heksana H. verticillata. Senyawa steroid diekstraksi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis dengan HCl 2 N dan dipartisi dengan n-heksana. Fraksi n-heksana hasil partisi diuji fitokimia dan dipisahkan senyawanya menggunakan KLT (Kromatografi Lapis Tipis). Identifikasi dilakukan menggunakan UV-Vis, FTIR, dan LC-MS/MS. Hasil penelitian menunjukkan ekstraksi maserasi menghasilkan rendemen 5,14%, sedangkan rendemen partisi n-heksana sebesar 47,95%. Pemisahan steroid menggunakan KLT analitik menunjukkan bahwa variasi eluen terbaik adalah n-heksana: etil asetat (4:1) dengan 12 noda, sedangkan pemisahan KLT preparatif menghasilkan 19 noda. Isolat steroid hasil KLT preparatif memiliki aktivitas antioksidan dengan EC50 5,109 ppm. Identifikasi senyawa steroid menggunakan UV-Vis menunjukkan bahwa panjang gelombang maksimum sebesar 203,9 dan 276 nm, sedangkan untuk identifikasi isolat steroid menggunakan FTIR menunjukkan gugus fungsi O-H, geminal dimetil, C=O, C=C, C-OH sekunder dan =C-H (alkena) yang diduga merupakan senyawa steroid. Hasil LC-MS/MS menunjukkan adanya senyawa steroid β-sitosterol.

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“…Ekstrak pekat metanol alga merah sebanyak 5 g ditambahkan 10 mL larutan HCl 2 N dan diaduk menggunakan magnetic stirer pada suhu ruang selama 1 jam. Selanjutnya, hidrolisat yang diperoleh ditambahkan NaHCO3 sampai pHnya netral (pH 7) [16].…”

Section: Hidrolisis Ekstrak Pekat Metanolunclassified

“…Partisi dilakukan tiga kali pengulangan dengan pelarut yang sama untuk memperoleh senyawa aktif yang lebih maksimal. Ekstrak hasil partisi kemudian dipekatkan dengan rotary evaporator sehingga diperoleh ekstrak pekat yang kemudian ditimbang beratnya dan ditentukan rendemennya [16].…”

Section: Partisi Ekstrak Dengan Petroleum Eterunclassified

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Variasi Komposisi Eluen pada Isolasi Steroid dan Triterpenoid Alga Merah Eucheuma cottonii dengan Kromatografi Kolom Basah

Madjid

Rahmawati

Fasya

2020

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Steroid and triterpenoid compounds in petroleum ether fractions of red algae Eucheuma cottonii were isolated by slurry column chromatography. Isolation was started with maceration extraction using methanol solvent. The methanol extract was hydrolyzed with HCl 2 N and partitioned using petroleum ether solvent. The presence of steroid and triterpenoid was determined by phytochemical test using Lieberman Burchard reagent. Petroleum ether fraction was separated by slurry column chromatography with the variation of eluent composition n-hexane: ethyl acetate 16:4; 17:3; 18:2. The isolates were monitored by analytical thin-layer chromatography (TLC) and the best result was identified using FTIR. Percent yields of methanol extract and petroleum ether fraction were 11.866% and 8.03%, respectively. The optimum eluent composition in column chromatography for separation was n-hexane : ethyl acetate (18:2) obtained 2 steroid and 3 triterpenoid fractions. FTIR analysis gives information about functional groups of –OH, C=C, C-O, CH2, -C(CH3)2forsteroids and functional groups of –OH, C=O, C=C, CH2, -C(CH3)2 for triterpenoid.Keywords: Eucheuma cottonii, column chromatography, steroid, triterpenoid, variation of eluent composition Senyawa steroid dan triterpenoid dalam fraksi petroleum eter alga merah Eucheuma cottonii telah diisolasi menggunakan kromatografi kolom basah. Isolasi dilakukan dengan cara ekstraksi maserasi menggunakan pelarut metanol. Ekstrak pekat metanol dihidrolisis dengan HCl 2 N dan dipartisi menggunakan pelarut petroleum eter. Senyawa steroid dan triterpenoid diuji fitokimia menggunakan reagen Lieberman Burchard. Fraksi petroleum eter dipisahkan menggunakan kromatografi kolom basah variasi komposisi eluen n-heksana:etil asetat dengan perbandingan 16:4; 17:3; dan 18:2. Hasil isolasi dimonitoring menggunakan kromatografi lapis tipis (KLT) analitik. Hasil monitoring yang terbaik diidentifikasi gugus fungsinya menggunakan FTIR. Hasil penelitian menunjukkan rendemen ekstrak metanol dan fraksi petroleum eter masing-masing sebesar 11,866% dan 8,03%. Pemisahan kolom dengan variasi komposisi eluen n-heksana:etil asetat terbaik adalah 18:2 dengan diperoleh 2 kelompok fraksi steroid dan 3 kelompok fraksi triterpenoid. Hasil analisis FTIR pada isolat steroid memberikan informasi gugus –OH, C=C, C-O, CH2, -C(CH3)2, sedangkan isolat triterpenoid memberikan informasi gugus –OH, C=O, C=C, CH2,-C(CH3)2. Gugus gem dimetil (-C(CH3)2)merupakan ciri khas rantai samping steroid ataupun triterpenoid.Kata kunci: Eucheuma cottonii, kromatografi kolom, steroid, triterpenoid, variasi komposisi eluen

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UJI FITOKIMIA DAN AKTIVITAS ANTIBAKTERI FRAKSI ETIL ASETAT, KLOROFORM DAN PETROLEUM ETER EKSTRAK METANOL ALGA COKLAT Sargassum vulgare DARI PANTAI KAPONG PAMEKASAN MADURA

Cited by 9 publications

References 1 publication

Gastroprotective effect of Canna edulis Ker. ethanolic extract in piroxicam-induced rats

Gastroprotective effect of Canna edulis Ker. ethanolic extract in piroxicam-induced rats

Aktivitas Antioksidan Isolat Steroid Hasil Kromatografi Lapis Tipis dari Fraksi n-Heksana Hydrilla verticillata

Variasi Komposisi Eluen pada Isolasi Steroid dan Triterpenoid Alga Merah Eucheuma cottonii dengan Kromatografi Kolom Basah

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