Uji Efek Antifungi Ekstrak Daun Biduri (Calotropis Gigantea L.) Terhadap Pertumbuhan Jamur Trichophyton Mentagrophytes

Abstract

The leaf of biduri (Calotropis gigantea L.) is one of the plants that can be used as antifungal. Biduri leaves are used as antifungals based on the chemicals contained in them such as tannins, flavonoid saponins and polyphenols. The fungus Trichophyton mentagrophytes can cause a skin disease characterized by the appearance of circular and scaly patches or small bubbles known as Tinea or ringworm. This fungus has a habitat (especially in tropical and wet climates) in soil, humans and animals. This study aims to determine the effectiveness of biduri leaf extract (Calotropis gigantea L.) in inhibiting the growth of the fungus Trichophyton mentagrophytes and to determine what is the optimal concentration of biduri leaf extract (Calotropis gigantea L.) to inhibit the growth of the fungus Trichophyton mentagrophytes. This research was carried out at the Microbiology Laboratory of the Tarbiyah Faculty of UIN Ar-Raniry and the Chemical Laboratory of the Chemical Science Faculty, Syiah Kuala University, Banda Aceh, from 26 April to 21 August 2018. Biduri leaves were extracted using the maceration method which were soaked for 5 days with 2 repetitions and evaporated with using a rotary evaporator to obtain extract as much as 30 ml blackish green and thick. Inhibition test against the fungus Trichophyton mentagrophtes was carried out using the disc diffusion method on Potato Dextrose Agar (PDA) media with concentrations of 20%, 35% and 50%. Ketoconazole antifungal was made in suspension form as a positive control and aquadest as a negative control. The results showed that the diameter of the inhibition zone obtained from biduri leaf extract at a concentration of 50% of 9.16 mm was included in the medium category, at a concentration of 35% of 8.66 mm was included in the medium category, 20% concentration of 8.16 mm was included. in the medium category, 11.83 mm of ketoconazole suspension was included in the strong category and 0 mm of aquadest which had no inhibitory power.

Effectiveness of Kemangi (Ocimum basilicum) Leaf Methanol Extract against Candida albicans Colonies

“…Tannins are dynamic compounds functioning as antifungals (Safrida, Mardiana & Husna, 2021). These antifungal compounds work by neutralizing enzymes involved in fungal invasion, damaging fungal cell membranes, inhibiting fungal enzyme systems, hence, they interfere with hyphae tip arrangement, and affecting nucleic acid and protein synthesis.…”

Phytochemical screening of three extraction process of Calotropis gigantea

“…This plant is commonly found in dry season areas, such as grasslands, mountains, active volcanic foothills, and beaches [6,7]. Aceh Province is one of the areas in Indonesia where C. gigantea plants grow wild and are considered weeds or nuisance plants [8,9]. Even though this plant contains many active compounds or secondary metabolites that have many health benefits [10,11].…”

“…The content of secondary metabolites in C. gigantea has differences, which are due to geographical factors, growing environment conditions, and genetics [18]. Several studies report that phytochemical screening of C. gigantea plant extracts has different results based on the different locations in Aceh province, such as geothermal areas [9], mountainous [13], and coastal areas [8]. Therefore, this study will conduct phytochemical screening of C. gigantea plant extracts that grow in the coastal area, the location of Alue Naga beach, Banda Aceh city, Aceh Province -Indonesia.…”