Uji Antibakteri Rimpang Gandasuli (Hedychium Coronarium) Terhadap Bakteri Staphylococcus Aureus Dan Escherichia Coli Dengan Perbandingan Metode Ekstraksi

Abstract

<p><em>Indonesia has various types of plants those have potential as medicine to overcome health problems in line with the expanding of traditional medicine industry. One example of plants from the Zingiberaceae family is Hedychium coronarium, which is not widely known by the community despite it has efficacy as an antibacterial which is not inferior to other Zingiberaceae family. The research was conducted to determine whether the Hedychium coronarium rhizome shown to have antibacterial activity against the growth of Staphylococcus aureus and Escherichia coli, knowing antibacterial compound that contained in Hedychium coronarium rhizome, also knowing the most effective extraction method to take the antibacterial compound from Hedychium coronarium rhizome. The gandasuli rhizome was extracted by maceration and reflux with 96% ethanol. Antibacterial activity test carried out by the agar diffusion method, and the analysis of antibacterial compounds with bioautography, and the results are monitored with vanillin-sulfuric acid. Maceration and reflux extract at a concentration of 90% has antibacterial activity with inhibition zones, each for 8 and 10 mm against Escherichia coli as well as 9 and 10 mm against Staphylococcus aureus. Bioautography results show a positive effect on the appearance of vanillin-sulfuric acid. Reflux extract has better antibacterial activity, while the antibacterial compound is an essential oil group.</em></p><p><strong> </strong><strong><em>Keywords:</em></strong><strong><em> </em></strong><em>Hedychium coronarium, antibacterial, Staphylococcus aureus, Escherichia coli, bioautography</em><em>.</em></p>

Antibacterial activity of ethanol extracts of hibiscus tiliaceus l. leaves from different extraction methods against Escherichia coli and Staphylococcus aureus

“…These antibacterial activities may, however, vary depending on the extraction technique used. For instance, temperature (i.e., hot or cold extraction) greatly affects the compounds or secondary metabolites drawn out of plant materials and the extract's antibacterial activity (Suhardiman et al, 2018). The Soxhlet and reflux are examples of hot extraction, while maceration and percolation are cold extractions (Patel et al, 2019).…”