UJI ANTI BAKTERI EKSTRAK DAUN JAMBU BIJI (Psidium guajava L) TERHADAP ZONA HAMBAT BAKTERI JERAWATPropionibacterium acnes SECARA IN VITRO

Afifi, Ruhana

doi:10.36465/jkbth.v17i2.259

Cited by 5 publications

(6 citation statements)

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“…Prebiotics from this Telang flower extract contain flavonoid compounds. Flavonoids in low concentrations are able to damage the cytoplasmic membrane causing damage to metabolites with the effect of inactivating the enzyme system of bacteria, while in high concentrations are able to damage the cytoplasmic membrane and precipitate cell proteins (Afifi & Erlin, 2017).…”

Section: Discussionmentioning

confidence: 99%

Differences in Minimum Inhibitory Concentrations of Probiotics and Prebiotics on Staphylococcus aureus

Yuliastuti,

Hermawati,

Islamy

2024

J. Prisma. Sains

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Currently, the use of antibiotics has increased sharply and use in the long term will have a bad impact on health. So the emergence of efforts in the form of making natural antibiotics by utilizing materials that are easily available in the environment. One of them is milk, where milk is a food product produced by farm animals, for example cows. This milk can be used as a probiotic. This probiotic is made by adding a bacterium and the bacterium that is often used is Lactobacillus casei. In addition to milk, the material that can be used is telang flower, which is one of the typical plants of Ternate, Maluku with purple, white, pink, and blue petals. Telang flower is often used as a traditional medicine because it has many benefits and content in it. Therefore, in this study Telang flower was used as a prebiotic tested on Staphylococcus aureus bacteria. The study was carried out in vitro by the method of laboratory experiments. The design used is Post Test control group only. The test is repeated at each concentration 3 times to obtain accurate results. The inhibition zone is measured using a caliper. The result of the inhibition zone formed from 3 repetitions is taken as the average value. The test results of the minimum inhibitory concentration of probiotics against Staphylococcus aureus bacteria showed results if at a concentration of 1.25% can form an inhibitory zone of 9.47 mm. While the minimum inhibitory concentration test on prebiotics against Staphylococcus aureus bacteria showed results at a concentration of 2.5% can form an inhibitory zone of 9.02 mm. This suggests that probitiks and prebiotics can be used as antibacterials, since at the lowest concentrations used they can form an inhibitory zone. So the higher the concentration of probiotics and prebiotics used, the inhibition zone formed will be greater.

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Section: Discussionmentioning

confidence: 99%

Differences in Minimum Inhibitory Concentrations of Probiotics and Prebiotics on Staphylococcus aureus

Yuliastuti,

Hermawati,

Islamy

2024

J. Prisma. Sains

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“…Staphylococcus aureus dapat masuk pada jaringan dan tinggal dalam waktu lama pada daerah infeksi karena organisme tersebut menghasilkan toksin (staphilotoksin, staphylococcal enterotoxin, dan exfoliatin) sehingga menimbulkan infeksi kulit. Staphylococcus aureus menyebabkan sindrom infeksi yang luas (Ruhana Afifi, 2017).…”

Section: Pendahuluanunclassified

“…Daun jambu biji (Psidium guajava L) mengandung senyawa aktif yang berperan sebagai antibakteri sehingga dapat digunakan sebagai alternatif antibiotik alami. Daun jambu biji mengandung senyawa metabolik sekunder seperti flavonoid, tannin, saponin, polifenol, minyak atsiri (eugenol), asam malat, asam ursolat, asam psidiolat, asam kratogolat, asam oleabolat, dan asam guajaverin (Ruhana Afifi, 2017). Berdasarkan penelitian-penelitian sebelumnya yang telah dilakukan menyebutkan bahwa saponin, tannin, dan flavonoid yang terkandung dalam daun jambu biji merupakan senyawa yang berkhasiat sebagai antibakteri (Handarni dkk., 2020).…”

Section: Pendahuluanunclassified

Uji Aktivitas Antibakteri Kombinasi Ekstrak Etanol Daun Jambu Biji Dan Daun Mangga Arumanis Terhadap S. Aureus

Widiastuti,

Fitriati,

Rahmawati

et al. 2023

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Daun mangga arummanis (Mangifera indica L.) dan daun jambu biji (Psidium guajava. L) terbukti memiliki aktivitas antibakteri dan antijamur. Staphylococcus aureus merupakan jenis bakteri patogen opportunistik yang dapat menyebabkan penyakit infeksi. Maserasi ekstrak dilakukan untuk menghilangkan adanya zat yang tidak dapat menghasilkan efek terapi. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun mangga arumanis dan daun jambu biji terhadap bakteri Staphylococcus aureus, serta kandungan senyawa flavonoid dengan metode KLT. Serbuk daun mangga arumanis dan serbuk daun jambu bji diekstraksi menggunakan pelarut etanol 70% dengan metode maserasi. Uji aktivitas antibakteri dilakukan dengan metode difusi cakram terhadap bakteri uji dengan seri konsentrasi 6,25%; 12,5%; dan 25% menggunakan perbandingan (1:1); (1:2); dan (2:1). Fase diam yang digunakan pada uji Kromatografi Lapis Tipis (KLT) adalah plat silika gel GF254 dengan fase gerak yang digunakan yaitu etil asetat : n-heksana (7:3) dengan pembanding senyawa flavonoid yang digunakan yaitu kuersetin dan untuk tannin dengan pembanding asam tanat. Analisis data yang diperoleh berupa diameter zona hambat, yang kemudian dianalisis secara statistik menggunakan One Way Anova. Hasil skrining fitokimia kombinasi ekstrak etanol daun jambu biji dan daun mangga arumanis mengandung senyawa metabolit sekunder berupa fenol, flavonoid, tanin, saponin, steroid, dan triterpenoid terkandung dalam daun mangga arumanis.

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“…Diameter zona hambat terbentuk karena terdapat senyawa bersifat antibakteri pada ekstrak daun pepaya seperti golongan flavonoid (kaemferol dan mirycetin) dan terdapat golongan fenolik (Agustina, 2019). Zona hambat dapat terbentuk dikarenakan adanya kemampuan ekstrak untuk berdifusi pada media agar sehingga menghambat pertumbuhan bakteri P. aeruginosa (Afifi, 2018).…”

Section: Hasil Dan Pembahasanunclassified

“…Zona hambat yang terbentuk menunjukkan pada media tidak lagi ditumbuhi oleh bakteri P. aeruginosa karena pada sekeliling cakram telah menyebar senyawa aktif metabolit sekunder dari ekstrak daun pepaya. Kandungan senyawa latex yang tedapat pada ekstrak daun pepaya di dalamnya terdapat enzim papain, alkaloid karpain, dan polifenol yang memiliki efek antibakteri (Afifi, 2018;Muamar, 2011).…”

Section: Hasil Dan Pembahasanunclassified

Aktivitas Antibakteri Ekstrak Etanol Daun Pepaya (Carica papaya Linn.) Terhadap Bakteri Pseudomonas aeruginosa

Kaban¹,

Gurning²,

Syahputra³

et al. 2022

INSOLOGI: Jurnal Sains dan Teknologi

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Pseudomonas aeruginosa is a Gram-negative bacterium that can cause nosocomial infections. The use of antibiotics in the treatment of non-adherent P. aeruginosa infections increases the risk of antibiotic resistance. Efforts to find new antibiotic compounds from plants continue to be carried out. Papaya leaves are widely spread among the public, which are often used as ingredients for vegetables and have antibacterial potential because they contain papain enzymes and a class of flavonoid compounds. The purpose of this study was to analyze the antibacterial activity of the ethanol extract of papaya leaves (Carica papaya Linn.) against P. aeruginosa by finding the value of the Minimum Inhibitory Concentration (MIC) and calculating the index activity value against the positive control. The method used in this research is Kirby-Bauer (disc diffusion method) with several variations of the test concentration. The results showed that the diameter of the inhibition zone at a concentration of 500 mg/mL was in the strong category with an inhibition zone diameter of 10.87 ± 0.06 mm. MIC at a concentration of 3.125 mg/mL. The index activity value was greatest at a concentration of 500 mg/mL with a value of 0.668. So it can be concluded that the ethanol extract of papaya leaves (Carica papaya Linn.) has inhibitory activity against Pseudomonas aeruginosa.

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UJI ANTI BAKTERI EKSTRAK DAUN JAMBU BIJI (Psidium guajava L) TERHADAP ZONA HAMBAT BAKTERI JERAWATPropionibacterium acnes SECARA IN VITRO

Cited by 5 publications

References 0 publications

Differences in Minimum Inhibitory Concentrations of Probiotics and Prebiotics on Staphylococcus aureus

Differences in Minimum Inhibitory Concentrations of Probiotics and Prebiotics on Staphylococcus aureus

Uji Aktivitas Antibakteri Kombinasi Ekstrak Etanol Daun Jambu Biji Dan Daun Mangga Arumanis Terhadap S. Aureus

Aktivitas Antibakteri Ekstrak Etanol Daun Pepaya (Carica papaya Linn.) Terhadap Bakteri Pseudomonas aeruginosa

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