Aktivitas Antibakteri Ekstrak Etanol Daun Pepaya (Carica papaya Linn.) Terhadap Bakteri Pseudomonas aeruginosa

Abstract

Pseudomonas aeruginosa is a Gram-negative bacterium that can cause nosocomial infections. The use of antibiotics in the treatment of non-adherent P. aeruginosa infections increases the risk of antibiotic resistance. Efforts to find new antibiotic compounds from plants continue to be carried out. Papaya leaves are widely spread among the public, which are often used as ingredients for vegetables and have antibacterial potential because they contain papain enzymes and a class of flavonoid compounds. The purpose of this study was to analyze the antibacterial activity of the ethanol extract of papaya leaves (Carica papaya Linn.) against P. aeruginosa by finding the value of the Minimum Inhibitory Concentration (MIC) and calculating the index activity value against the positive control. The method used in this research is Kirby-Bauer (disc diffusion method) with several variations of the test concentration. The results showed that the diameter of the inhibition zone at a concentration of 500 mg/mL was in the strong category with an inhibition zone diameter of 10.87 ± 0.06 mm. MIC at a concentration of 3.125 mg/mL. The index activity value was greatest at a concentration of 500 mg/mL with a value of 0.668. So it can be concluded that the ethanol extract of papaya leaves (Carica papaya Linn.) has inhibitory activity against Pseudomonas aeruginosa.

Antibacterial Activity and Determination of Total Phenol and Flavonoid of Carica papaya L. Ethanol Extract

“…Following their cleansing and rinsing, papaya leaves are dried. The dried papaya leaves are ground to a fine powder [12]. Using a rotary evaporator, evaporate at 40°C until a concentrated extract is obtained [12].…”

Antibacterial Activity Ethanolic Extract of Ocimum basilicum L. Leaves in Inhibiting the Growth of Escherichia coli and Pseudomonas aeruginosa

“…As much as 200 g of kemangi leaf powder was soaked in 1500 mL of ethanol for 5 days, then filtered and macerated again for 2 days with 500 mL of ethanol 7 . The extract obtained was concentrated using a rotary vacuum evaporator 7,8 .…”

“…A condensed extract of kemangi leaves was weighed at 1 g and then dissolved in 2 mL of DMSO for a concentration of 500 mg/mL. Then it was diluted with various concentrations of 250, 100, 50, 25, 12.5, 6.25, and 3.125 mg/mL 8,11 .…”

Phytochemical Screening and Antibacterial Activity Ethanolic Extract of Solanum mauritianum Scop Leaves Against Staphylococcus aureus and Pseudomonas aeruginosa

“…Glass were used in this study were sterilized by the dry heat method using an oven at 170ᵒC for 1 hour. The media used in this study were sterilized using the wet heat method by autoclaving at 121ᵒC for 15 minutes [5], [12].…”

“…The ethanol extract was weighed up to 5 g, then dissolved in dimethylsulfoxide (DMSO) to 10 mL (concentration 500 mg/mL), then diluted with 400, 300, 200, 100, 80, 60, 40 (mg/mL) [12], [13].…”