Uji Antagonisme Beberapa Fungi Endofit pada Tanaman Kentang  terhadap Fusarium oxysporum secara In Vitro

Izzatinnisa', Izzatinnisa'; Utami, Ulfah; Mujahidin, Ahmad

doi:10.26740/jrba.v2n1.p18-25

Cited by 6 publications

(4 citation statements)

References 2 publications

(2 reference statements)

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“…Fusarium merupakan jenis jamur patogen yang sering menyerang tanaman kentang. Hal ini disampaikan pada penelitian Sari et al (2016) dan Izzatinnisa et al (2020), bahwa salah satu kendala yang dijumpai dalam produksi kentang di Indonesia ialah penyakit layu dan busuk kering umbi kentang yang disebabkan oleh cendawan patogen Fusarium spp. Keterlibatan Fusarium spp.…”

Section: Pendahuluanunclassified

Aktivitas Cendawan Antagonis Trichoderma terhadap Fusarium dari Tanaman Kentang di Kecamatan Pakis Kabupaten Magelang

Purwantisari¹,

Mahardhika²,

Naufal³

et al. 2023

Bioscientist j. ilm. bosbiol.

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Potato is one of the common plants cultivated in Indonesia and used as a food ingredient besides rice. One of the pathogens that can attack potatoes is Fusarium. Farmers usually use synthetic fungicides to inhibit Fusarium infection, but this method is not environmentally friendly. One of the countermeasures for Fusarium can be done with the antagonistic fungus Trichoderma. This study aims to examine the antagonistic of Trichoderma spp. toward isolate Fusarium spp. from potato plants showing wilting symptoms. The method was carried out by isolating the fungus from potatoes with wilting symptoms, identifying it, and testing 4 Trichoderma strains using a double culture method. The fusarium obtained was identified as F. begoniae, F. foetens, F. mooniliforme, and F. oxysporum. A total of 2 Trichoderma strains originating from litter and trash cans were able to inhibit all Fusarium tests on day 2 and achieve 100% growth inhibition on day 4, while the other 2 Trichoderma strains only showed inhibition on day 4 to day 7. Further tests are needed to understand the mechanism of Fusarium attack, and to identify the antifungal compounds produced by Trichoderma.

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Section: Pendahuluanunclassified

Aktivitas Cendawan Antagonis Trichoderma terhadap Fusarium dari Tanaman Kentang di Kecamatan Pakis Kabupaten Magelang

Purwantisari¹,

Mahardhika²,

Naufal³

et al. 2023

Bioscientist j. ilm. bosbiol.

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“…Pengamatan secara mikroskopik menunjukkan adanya hifa bersepta. Berdasarkan ciri makroskopik dan mikroskopik tersebut maka dapat diketahui bahwa isolat IJP2 termasuk ke dalam genus Neoscytalidium sp [11].…”

Section: Karakterisasi Isolat Jamur Endofitunclassified

Isolasi dan Karakterisasi Jamur Endofit Daun Belimbing Hutan (Cnestis palala (Lour.) Merr)

Azzahra,

Naspiah,

Febrina

2023

Proc. Mul. Pharm. Conf.

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Belimbing hutan (Cnestis palala (Lour.) Merr) is recognized as a distinctive plant in East Kalimantan, commonly used by the local community for treating internal diseases, postpartum conditions, abdominal pain, and urinary tract disorders. The benefits of belimbing hutan cannot be separated from the compounds contained in it. However, the limited population makes exploration of this plant hampered. Endophytic fungi are an interesting alternative for exploring secondary metabolites from this plant. To date, there have been no scientific reports on endophytic fungus of belimbing hutan. This research successfully isolated and characterized three isolates from the leaves of belimbing hutan with isolate codes IJP1 exhibiting white colonies with a cotton-like texture, IJP2 colonies are white and fiber-shaped, IJH1 with green colonies accompanied by concentric circles. The diversity of endophytic fungi in belimbing hutan leaves opens up possibilities for discovering potential compounds from the extracts of these fungi. Keywords: Cnestis palala (Lour.) Merr, Endophytic Fungi, Isolation, Characterization Abstrak Belimbing hutan (Cnestis palala (Lour.) Merr) dikenal sebagai tanaman khas Kalimantan Timur yang umumnya digunakan sebagai obat penyakit dalam, obat pasca melahirkan, sakit perut dan gangguan saluran kemih oleh masyarakat sekitar. Khasiat dari belimbing hutan ini tidak terlepas dari senyawa yang terkandung di dalamnya. Namun, populasi yang terbatas membuat eksplorasi tumbuhan ini menjadi terhambat. Jamur endofitik merupakan salah satu alternatif menarik untuk mengeksplorasi metabolit sekunder dari tanaman ini. Sepanjang pengetahuan, belum ada laporan ilmiah mengenai jamur endofitik belimbing hutan. Penelitian ini berhasil mengisolasi dan mengkarakterisasi tiga isolat dari daun belimbing hutan dengan kode isolat IJP1 yang memiliki karakteristik koloni berwarna putih dan tekstur halus seperti kapas, IJP2 koloni berwarna putih dan berbentuk serabut, IJH1 koloni berwarna hijau disertai lingkaran konsentris. Beragamnya jamur endofitik daun belimbing hutan membuka peluang ditemukannya senyawa potensial dari ekstrak jamur endofitik daun belimbing hutan. Kata Kunci: Cnestis palala (Lour.) Merr, Jamur Endofit, Isolasi, Karakteristik

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“…Selanjutnya diletakkan pada tissue steril sampai kering. Isolat selanjutnya diletakkan dalam cawan petri yang berisi media PDA, masingmasing cawan berisi 2 potongan daun, kemudian diinkubasi selama 7 hari pada suhu 25-30 o C (Izzatinnisa et al, 2020).…”

Section: Isolasi Jamur Patogenunclassified

KARAKTERISASI DAN UJI POTENSI JAMUR ENDOFIT PADA DAUN BAWANG MERAH (Allium ascalonicum L.) SEBAGAI PENGENDALI PATOGEN Fusarium sp. dan Alternaria sp.

Mita Erliza,

Syafrina Sari Lubis

2023

kenanga

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Shallot (Allium ascalonicum L.) is a horticultural commodity that has high economic value and is needed every day by the community. Attack by pets and pathogens on shallot cultivation cause a decrease in crop productivity. The pathogen that often attacks shallot plants is Fusariumsp. and Alternaria sp. This study aims to obtain endophytic fungi from shallot leaves that can control Fusarium sp. and Alternaria sp. The endophytic fungus isolation method uses healthy shallot leek samples while the pathogen isolation uses unhealthy leaves. The antagonism potency test was conducted using the dual culture method with an incubation time of 7 days at 25-30oC. Based on the results of the study, there were 4 isolates of endophytic fungi on shallot leaves, namely EA1 isolates (Aspergillus niger), EA2 isolates, EA3 isolates (Clasdoporium sp.) and EA4 isolates (Phyllosticta sp.). The results of the antagonist test showed that the endophytic fungus EA1 was able to inhibit the fungus Fusarium sp. and Alternaria sp. maximally (strong) with an average value of Fusarium sp. of 62.72% while Alternaria sp. 67.83 %. The endophytic fungus EA2 was also able to inhibit both of these pathogens but it was moderately categorized with an average value of Fusarium sp. 52.11 % and Alternaria sp. 53.28%. The mechanisms of inhibition are competition and hyperparasitism.

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Uji Antagonisme Beberapa Fungi Endofit pada Tanaman Kentang terhadap Fusarium oxysporum secara In Vitro

Cited by 6 publications

References 2 publications

Aktivitas Cendawan Antagonis Trichoderma terhadap Fusarium dari Tanaman Kentang di Kecamatan Pakis Kabupaten Magelang

Aktivitas Cendawan Antagonis Trichoderma terhadap Fusarium dari Tanaman Kentang di Kecamatan Pakis Kabupaten Magelang

Isolasi dan Karakterisasi Jamur Endofit Daun Belimbing Hutan (Cnestis palala (Lour.) Merr)

KARAKTERISASI DAN UJI POTENSI JAMUR ENDOFIT PADA DAUN BAWANG MERAH (Allium ascalonicum L.) SEBAGAI PENGENDALI PATOGEN Fusarium sp. dan Alternaria sp.

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