UJI AKTIVITAS PENGHAMBATAN ENZIM α-GLUKOSIDASE EKSTRAK ETANOL BIJI BUAH ALPUKAT SANGRAI (Persea americana Mill.) SECARA IN VITRO

Abstract

It is known that the seeds of avocado (Persea americana Mill.) are one part of the avocado widely used to treat various diseases, including diabetes. Based on the previous research, avocado seeds contain flavonoids with the potency as inhibitors of the enzyme α-glucosidase. The research aimed to determine the potency of ethanol extracted by meceration using 96% ethanol. The inhibitory activity was assayed using ELISA (Enzyme-Linked Immunosorbant Assay) reader measured at the wavelength of 405 with an acarbose comparator. The results showed that the ethanol extract of avocado seeds had an inhibitory activity included in the active category based on IC50 value of 34.300 µg/mL. Meanwhile, the acarbose had inhibition activity included in the very active category with an IC50 value of 0.253 µg/mL.

INHIBITORY EFFECT OF CURCUMIN INCORPORATED IN CHITOSAN NANOPARTICLE ON Α-Amylase AND Α-Glucosidase ACTIVITIES

“…Inhibitory activity of α-glucosidase can be observed from the decrease in absorption at 305 nm, which indicates the absorption of p-nitrophenol formed. The substrate used was PNPG as a model that represents carbohydrates in the body, where the enzyme will break down the substrate into glucose and p-nitrophenol [5]. The result of this study showed that nano curcumin has a smaller IC50 value of 3.95 µg/ml compared to acarbose of 4.11 µg/ml, it is shown in fig.…”

“…Then, 250 µl of 1% starch solution as a substrate and 250 µl iodine was added sequentially. Absorbance was measured at 595 nm by using UV-Visible spectrophotometer Thermo Scientific-Genesys 10S UV-Vis [5].…”

“…Curcumin is the main yellow polyphenolic compound derived from the rhizome of turmeric (Curcuma longa). According to several research, curcumin has pharmacological activity such as anticancer, anti-inflammatory, antioxidant and anti-hyperglycemic by inhibiting α-amylase and α-glucosidase enzymes [5]. However, curcumin is classified into class IV of Biopharmaceutical Classification System (BCS) based on its poor water solubility (4.375 g/ml) and low bioavailability (1,498±0.402 µg h/ml) [6].…”