Uji Aktivitas Ekstrak Buas-Buas (Premna Serratifolia Linn) Sebagai Anti Kolesterol Secara in Vitro

Abstract

ABSTRAKDaun Buas-buas (Premna serratifolia Linn) dipercaya oleh masyarakat dapat menurunkan kolesterol solusi obat herbal alternatif ditengah mahal dan efek samping buruk yang ditimbulkan obat sintetik. Penelitian dilakukan dengan tahapan daun Buas-buas (Premna cordiflora Linn) dikeringkan, dihaluskan dan dimaserasi menggunakan etanol, kloroform, dan n-heksan. Ekstrak yang diperoleh dari evaporasi, ditimbang dan disimpan dalam desikator. Uji aktivitas antikolesterol secara In Vitro dimulai dengan penentuan panjang gelombang maksimum larutan baku kolesterol dengan spektrofotometer UV-Visible. Dilanjutkan dengan pembuatan kurva standart dengan konsentrasi larutan kolesterol 0.5; 0,75; 1; 1,25; dan 1,5 mL larutan kolesterol 1000 ppm ditambahkan etanol 95% hingga volume 5 mL serta diukur absorbansinya. Pengukuran kadar koleterol setalah diberikan ekstrak etanol, kloroform, dan n-heksan daun Buas-buas dengan cara 0,5; 1; 1,5; 2; dan 2,5 mL ditambahkan 5 mL larutan kolesterol dengan konsentrasi 100 ppm. Selanjutnya diukur absorbansi pada panjang gelombang maksimum yang diperoleh pada panjang gelombang maksimum kolesterol. Hasil penelitian menunjukkan ekstraksi dengan pelarut etanol menghasilkan rendemen terbanyak. Ekstrak Premna serratifolia Linn dapat berfungsi sebagai anti kolesterol. Penurunan absorbansi larutan standar kolesterol 100 ppm terdapat pada penmbahan 0,5 mL ekstrak kloroform. ABSTRACTPremna Serratifolia Linn is believed to reduce cholesterol and as an alternative herbal medicine solution among the cost of medicine and the side effects caused by synthetic drugs. This study was conducted in several phases by using Premna Serratifolia Linn which was drained, mashed, and macerated using ethanol, choloform, and n-heksane. Extract obtained from evaporation, then whighed, and stored in a desiccators. Anti-cholesterol activity was tested test by using in vitro : began with determination of the maximum wavelength of the cholesterol standard solution with a UV-Vis spectrophotometer and continued by manufacturing the standard curve with the cholesterol concentration of 0.5; 0.75; 1; 1.25; and 1.5 mL and 1000 ppm cholesterol solution. Furthermore, maximum absorbance wavelength was measured in order to obtain the maximum wavelength of the cholesterol. The study reveled that the solvent extraction of ethanol produced the largest rendement. The extract Premna Serratifolia Linn is functioned as an anti-cholesterol. In addition, the absorbed reduction of 100 ppm cholesterol standard solution found in the addition of 0.5 mL choloform extract.

Phytochemistry and Pharmacological Potentials of Premna serratifolia L.: Traditional Medicinal Plant Used by Local People in Kalimantan

“…While the lowest absorbance was indicated by the addition of 0.5 ml of chloroform extract in 5 ml of standard solution with a concentration of 100 ppm, which was 2.735. This decrease in absorbance shows that chloroform extract with the addition of 0.5 ml is the most effective as an anti-cholesterol 19 .…”

“…In vitro 19 Leaves The greatest absorbance of the extract with either chloroform, ethanol, and n-hexane as solvents was indicated by the addition of 2.5 ml of chloroform extract in 5 ml of standard solution with a concentration of 100 ppm, which was 2.998.…”

“…Tannins can affect the color of the supernatant solution that will be absorbed. The addition of the extract also makes the supernatant solution dark so that the absorbance obtained is high due to the large number of molecules that interact with light 19 .…”

Profiling Senyawa Metabolit Sekunder Fraksi Aktif Antihiperkolesterolemia Dari Herba Ketumpang Air (Peperomia pellucida L)

“…Pengobatan melalui farmakologis yaitu dengan menggunakan obat-obatan seperti penggunaan golongan statin. Pengobatan farmakologis menggunakan obatobatan sintesis dapat menimbulkan berbagai efek samping seperti nyeri pada otot dan persendian hingga diare (Hadiarti, 2017). Salah satu upaya mengatasainya adalah dengan penggunaaan obat berasal ekstrak tumbuhan.…”

Potensi Inhibisi Enzim α-Glukosidase Dari Ekstrak Kulit Buah Labu Air (Lagenaria siceraria) Sebagai Antidiabetes

“…Akarbosa dapat menghambat kerja enzim α-glukosidase secara kompetitif. Akarbosa dapat digunakan sebagai obat oral dalam terapi pada penderita diabetes melitus tipe 2 [20]. Penghambatan enzim α-glukosidase oleh akarbosa akan dapat menunda absorbsi dan digesti glukosa.…”