Uji Aktivitas Antioksidan Ekstrak Daun Karet Kebo (Ficus Elastica) dengan Metode Peredaman Radikal Bebas Dpph (1,1-Diphenyl-2-Picrylhydrazil)

Abstract

Ficus elastica leaf is one of the Ficus species assumed to be rich in polyphenols so that it can act as an antioxidant. The research aimed to determine the antioxidant activity and IC50 value of methanol and water extracts of rubber leaf. In this research, the extraction was conducted by maceration using methanol and obtained the yield value of 7,03%, while the infundation using water obtained the yield value of 2,82%. The antioxidant activity test was conducted by DPPH free radical scavenging (1,1-diphenyl-2-phycrilhydrazyl) using a quercetin comparator. The qualitative tests were conducted by TLC method using dicloromethane:methanol eluent with a ratio of 99:1 for methanol extracts, and a ratio of 95:5 for water extracts, where all extracts of both methanol and water positively container antioxidants characterized by the formation of yellow spots with a purple background on TLC plates. The results of the quantitative test shower that the methanol extract had medium antioxidant activity with an IC50 value of 78,39 µg/mL, and water extract had an inactive antioxidant activity with an IC50 value of 319,11 µg/mL. While quercetin was classified as a very strong antioxidant with an IC50 value of 7,62 µg/mL.

THE EFFECT OF VARIATIONS IN ROASTING TEMPERATURE ON YIELD AND PERCENTAGE OF INHIBITION OF DPPH RADICAL REDUCTION IN CANDLENUT OIL THE UV-Vis SPECTROPHOTOMETRIC METHOD

“…Metode DPPH merupakan metode yang cepat, murah, dan sederhana. Prinsip pengujian aktivitas aktioksidan dengan metode DPPH dengan adanya donor atom hidrogen dari senyawa antioksidan ke DPPH radikal sehingga dihasilkan DPPH-H sebagai hasil reduksi dari DPPH (Handayani et al, 2020). DPPH berwarna ungu, disebabkan adanya perpindahan ditunjukkan oleh pita serapan dengan panjang gelombang 515 nm, karena adanya aktivitas antioksidan, ketika digabung dengan senyawa yang dapat memberikan atom hidrogen, terjadi peningkatan bentuk tereduksi dari DPPH yang mengakibatkan perubahan warna ungu menjadi warna kuning pucat (pudar) (Agustina et al, 2020).…”

PENENTUAN AKTIVITAS ANTIOKSIDAN INFUDASI AKAR BAJAKAH TAMPALA (Spatholobus littoralis Hassk) DAN KALALAWIT (Uncaria Gambir Roxb) DENGAN METODE DPPH

“…Selanjutnya identifikasi terpenoid menggunakan pereaksi Lieberman-Burchard (20 tetes asam asetat anhidrat dan 1 tetes asam sulfat pekat). Hasil berwarna hijau kebiruan adalah senyawa golongan steroid dan larutan membentuk cincin coklat adalah triterpenoid (Handayani, Kurniawati and Abdul Rasyid, 2020).…”

“…Selanjutnya, untuk menganalisis aktivitas antioksidan sampel, digunakan persamaan regresi linier untuk mencari nilai Inhibitory Concentration (50%) IC50. Menurut Phongpaichit suatu senyawa dikatakan sebagai antioksidan sangat kuat apabila memiliki nilai IC50 < l0 μg/mL, antioksidan kuat apabila nilai IC50 antara 10-50 μg/mL, sedang apabila nilai IC50 berkisar antara 50-100 μg/mL, lemah apabila nilai IC50 antara 100-250 μg/mL dan tidak aktif apabila IC50 diatas 250 μg/mL (Handayani, Kurniawati and Abdul Rasyid, 2020). Berdasarkan hasil penelitian pada akar bajakah, nilai IC50 ekstrak infusa bajakah Tampala adalah 70,81 μg/ml, sedangkan nilai IC50 infusa jenis Kalalawit adalah 71,77 μg/ml.…”

Toxicity Activity and Total Phenolic Content of Soursop Leaves from Three Regions in South Sulawesi, Indonesia

“…Using probit analysis, the lethal concentration (LC50) was determined. Calculating the LC50 value employs a regression line derived by plotting the concentration of the substance towards the percentage of death on a probit scale (Handayani, Kurniawati, and Abdul Rasyid 2020).…”

“…Soursop contains natural phenolic compounds that have potential as antioxidants, have bioactivity as drugs, and contain annonaceous acetogenins, which have cytotoxic potential (Puspitasari et al 2016). Cytotoxic compounds are compounds that can be toxic to inhibit and stop the growth of cancer cells (Handayani, Kurniawati, and Abdul Rasyid 2020).…”