Uji Aktivitas Antioksidan dan Daya Hambat Enzim Tirosinase Ekstrak Etanol Azolla filiculoides Lam.

Abstract

Azolla (Azolla filiculoides Lam.) merupakan tanaman paku air dari famili Azollaceae. Azolla filiculoides digunakan sebagai pakan alami dengan ketersediaannya melimpah di alam yang belum termanfaatkan secara optimal. Tanaman ini dapat bersimbiosis dengan Cyanobacteria. Azolla memiliki senyawa bioaktif yang berfungsi sebagai antioksidan dan inhibitor tirosinase. Tujuan dari penelitian ini adalah untuk menguji aktivitas antioksidan menggunakan metode DPPH (2.2-difenil 1-pikrihidrasil ) dan daya hambat tirosinase pada ekstrak etanol Azolla filiculoides. Ekstraksi menggunakan metode maserasi dan pelarut etanol 96%. Hasil uji fitokimia menunjukkan bahwa ekstrak etanol Azolla mengandung senyawa flavonoid, tanin, saponin, steroid dan triterpenoid. Kandungan fitokimia tersebut berpotensi sebagai antioksidan dan inhibitor tirosinase, terutama senyawa flavonoid dan tanin. Uji aktivitas antioksidan menunjukkan bahwa ekstrak etanol Azolla filiculoides memiliki aktivitas antioksidan yang kuat dengan nilai IC50 sebesar 23.00 ppm. Uji aktivitas inhibisi tirosinase ekstrak azolla menunjukkan daya hambat yang lebih efektif pada aktivitas diphenolase (IC50 996.60 ppm) dibandingkan aktivitas monophenolase (IC50 1893.09 ppm).

In Vitro Evaluation of Purple Sweet Potato Leaf Extract (Ipomoea batatas) as a Tyrosinase Inhibitor and Malondialdehyde Formation Inhibitor

“…The ability of flavonoids as antioxidant compounds is due to their properties as good acceptors against free radicals. Phenolic and flavonoid compounds from plants have the ability to act as antioxidants because they are able to scavenge reactive oxygen species (ROS) which produce free radicals (Prasetyo, 2021). From the results of the identification of samples with ethanol solvent which contain secondary metabolite compounds in the form of flavonoids and the results of the Tukey statistical test, it can be concluded that the ethanol extract and ascorbic acid as a positive control are not significantly different and the ethanol solvent is not significantly different from the water solvent.…”

“…The standard used in the calculation of MDA levels is tetramethoxypropane (TMP). Standard TMP in acidic conditions can be hydrolyzed to produce semiacetal and methanol, the hemiacetal formed will then decompose into methanol and aldehyde which can react with TBA (Prasetyo, 2021;Wresdiyati et al, 2002). The resulting linear regression equation for measuring the standard curve is y = 0.0406x -0.0133 with a value of r = 0.9961.…”

The Antityrosinase Activity of Mitragyna speciosa Korth Leaf Ethanolic Extract

“…Senyawa steroid memiliki aktivitas antitirosinase dengan mekanisme mengikat beberapa situs dari enzim tersebut kecuali situs katalitik. Sedangkan, kuinon memiliki aktivitas antitirosinase yang mekanismenya berkaitan dengan gugus fungsi yang melekat pada kerangkanya (Prasetyo, 2021).…”

The influence of extraction temperature and time on antiradical activity and total phenolic extract of Ceciwis

“…External sources of free radical generation include stress, ozone radiation, pollution, pesticides, and industrial chemicals, all of which can harm molecules, proteins, lipids, RNA, and DNA in the body [1,2]. Free radicals can cause cellular oxidative damage, which diminishes the body's defense system and makes a significant contribution to diseases such as diabetes, hypercholesterolemia, cancer, arthritis, arteriosclerosis, degenerative neuro-diseases, and premature aging [1,2]. As a result, antioxidants are required by our bodies to protect us from free radical damage According to Halliwell in [3], antioxidants are substances that inhibit, prevent, and completely remove oxidative damage to target molecules.…”