Uji aktivitas antagonistik beberapa isolat Bacillus spp. terhadap penyakit layu bakteri (Ralstonia solanacearum) pada beberapa varietas tomat dan identifikasinya

Saputra, Rachmad; Arwiyanto, Triwidodo; Wibowo, Arif

doi:10.13057/psnmbi/m010525

Cited by 5 publications

(4 citation statements)

References 6 publications

(7 reference statements)

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“…Hal tersebut sesuai dengan pendapat Pitasari dan Ali (2018) yang menyatakan bahwa perbedaan daya hambat yang dihasilkan oleh bakteri dapat terjadi karena adanya perbedaan jenis dan jumlah metabolit sekunder bersifat menghambat yang dihasilkan oleh masing-masing isolat. Saputra et al (2015) menambahkan bahwa perbedaan diameter daya hambat juga dapat dipengaruhi oleh perbedaan fisiologis bakteri dalam memanfaatkan nutrisi pada media. Beberapa bakteri dapat memanfaatkan nutrisi untuk menghasilkan senyawa antibiotik dan beberapa bakteri memanfaatkan nutrisi untuk pertumbuhannya.…”

Section: Potensi Dan Persentase Daya Hambat Bakteri Endofit Asal Tumb...unclassified

UJI POTENSI DAN MEKANISME ANTAGONIS BAKTERI ENDOFIT ASAL TUMBUHAN LIAR SEBAGAI AGENS HAYATI PENGENDALIAN Fusarium oxysporum SECARA IN-VITRO

RAHIMI

KHAERUNI²,

BANDE³

et al. 2022

Agroteknos

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This research motivated by the Fusarium oxysporum which is a soil borne fungus that can infect cultivated plants. F. oxysporum is a facultative parasite that is part of its life as a saprophyte and becomes a parasite if there is a host plant. This study aims to determine the potential of endophytic bacteria from wild plants as biological agents control of F. oxysporum in-vitro. This research was conducted at the Laboratory of Plant Protection, Phytophatology Unit, Faculty of Agriculture, Halu Oleo University from September 2020 to March 2021. The results showed that endophytic bacteria isolated from wild plants such as as Jamaica cherry/kersen (Muntingia calabura), Suruhan (Paperomia pellucida), and Markisa Hutan/Rambusa (Passiflora foetida) have the potential as biological agents to control F. oxysporum in vitro, with the two best isolates, namely ASR-7(3) and BK-3(2) which were to inhibit the growth of F. oxysporum through the mechanism of antibiosis and competition, produced enzyme bioactive compounds (amylase and protease) which degrade of pathogenic fungi, and are able to produce volatile compounds.

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Section: Potensi Dan Persentase Daya Hambat Bakteri Endofit Asal Tumb...unclassified

UJI POTENSI DAN MEKANISME ANTAGONIS BAKTERI ENDOFIT ASAL TUMBUHAN LIAR SEBAGAI AGENS HAYATI PENGENDALIAN Fusarium oxysporum SECARA IN-VITRO

RAHIMI

KHAERUNI²,

BANDE³

et al. 2022

Agroteknos

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“…Suspensi bakteri patogen (0,2 ml suspensi air steril bakteri patogen dalam 4 ml 0,6 % agar air pada suhu 45°C), dituang pada permukaan medium tersebut lalu diinkubasi selama 24 jam pada suhu ruang. Terbentuk berupa zona hambatan diukur sesuai zona bening (Saputra et al 2015). Pengukuran dilakukan dengan rumus (Izza et al 2018…”

Section: Uji Antagonis Bacillus Spp Terhadap B Glumaeunclassified

Identifikasi penyebab penyakit busuk bulir bakteri pada tanaman padi (Oryza sativa) dan pengendaliannya menggunakan isolat Bacillus spp. secara in vitro

Isnaeni

Masnilah

2020

J. Prot. Trop. Crop

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Bacterial grain rot disease in rice plant is a new disease in Indonesia. The disease can reduce rice production up to 40% in severe disease attack. Morphological and physiological identification of these pathogens can be useful as a strategy of disease control. Control of this disease using Agent Biological Control is a right step and eco-friendly. The research was conducted in July until November 2018 at Plant Quarantine Laboratory of Agricultural Quarantine Center Surabaya. Design of experiment used on pathogenic identification is descriptively and analytically while in strategy of diseases control used are Randomized Complete Design method of single factor as many as 5 treatment of Bacillus spp. such as; Ba1: Bacillus spp. isolate 1, Ba2: Bacillus spp. isolate 2, Ba3: Bacillus spp. isolate 3, Ba4: Bacillus spp. isolate 4, and Ba5: Bacillus isolate spp. 5. Research result showed that the pathogen causing bacterial grain rot in Rice is Burkholderia glumae with bacterial characteristic are Gram negative, aerobic, not fluorescent on Kings B medium, mesophyll, isotonic, can hydrolysis arginine and can produce enzyme of gelatinase and (2) Bacillus spp. Isolate which consistent to inhibiting B. glumae in vitro is Ba-1 with a mean inhibition of 17.88 mm and a variety of resistance mechanisms, namely bacteriostatic and bactericidal Isolate of Bacillus spp Keywords: Bacterial grain rot rice; Bacillus spp; Burkholderia glumae

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“…Ralstonia solanacearum is a plant pathogen that causes bacterial wilt disease in the Solanaceae family and other plant species (Saputra et al 2015). The occurrence of bacterial wilt disease starts from the entry of R. solanacearum through wounds or natural openings on plant roots.…”

Section: Introductionmentioning

confidence: 99%

Physio-biochemical, molecular characterization, and phage susceptibility of Ralstonia pseudosolanacearum associated with tomato (Solanum lycopersicum) and eggplant (Solanum melongena)

2022

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Abstract. Nurdika AAH, Arwiyanto T, Sulandari S. 2022. Physio-biochemical, molecular characterization, and phage susceptibility of Ralstonia pseudosolanacearum associated with tomato (Solanum lycopersicum) and eggplant (Solanum melongena). Biodiversitas 23: 5149-5158. Ralstonia solanacearum is a plant pathogen that causes bacterial wilt diseases in various plant species. The high diversity of these bacteria strains is the basis for their grouping into the R. solanacearum species complex (RSSC). RSSC associated with tomato and eggplant in Indonesia are commonly known as R. solanacearum. It is necessary to characterize R. solanacearum which infects tomatoes and eggplant according to the latest classification. The high diversity of strains also affects their susceptibility to bacteriophages as host-specific biocontrol agents. This research was conducted by characterizing R. solanacearum isolates from different locations based on their physio-biochemical properties, biovar, virulence, phylotype, sequevar, and susceptibility to 12 bacteriophage isolates. The phylotype identification was carried out using multiplex polymerase chain reaction with several specific primers for R. solanacearum. Amplification and sequencing based on the egl gene region were carried out to determine the sequevar of R. solanacearum isolates. As a result, isolates RS18, RS19, RS23, and RS24 had morphological, physio-biochemical, and biovar characteristics according to Ralstonia pseudosolanacearum phylotype I, biovar 3. The two most virulent isolates, RS19 and RS24 were identified as sequevar 14. RS19 and RS24 isolates were susceptible to 7 of the 12 bacteriophage isolates used in this study. Variations of bacteriophage isolates resulted in different plaque morphology which could be attributed to R. pseudosolanacearum susceptibility and bacteriophage virulence.

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Uji aktivitas antagonistik beberapa isolat Bacillus spp. terhadap penyakit layu bakteri (Ralstonia solanacearum) pada beberapa varietas tomat dan identifikasinya

Cited by 5 publications

References 6 publications

UJI POTENSI DAN MEKANISME ANTAGONIS BAKTERI ENDOFIT ASAL TUMBUHAN LIAR SEBAGAI AGENS HAYATI PENGENDALIAN Fusarium oxysporum SECARA IN-VITRO

UJI POTENSI DAN MEKANISME ANTAGONIS BAKTERI ENDOFIT ASAL TUMBUHAN LIAR SEBAGAI AGENS HAYATI PENGENDALIAN Fusarium oxysporum SECARA IN-VITRO

Identifikasi penyebab penyakit busuk bulir bakteri pada tanaman padi (Oryza sativa) dan pengendaliannya menggunakan isolat Bacillus spp. secara in vitro

Physio-biochemical, molecular characterization, and phage susceptibility of Ralstonia pseudosolanacearum associated with tomato (Solanum lycopersicum) and eggplant (Solanum melongena)

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