UHPLC Separation of Structurally Diverse Markers in Fangchi Species

Kim, Jihee; Sim, Hee‐Jung; Lee, Kang Ro; Hong, Jongki

doi:10.5012/bkcs.2013.34.3.975

Cited by 3 publications

(5 citation statements)

References 18 publications

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“…The simultaneous analysis of several alkaloids with different skeletons present in Fangchi species is not an easy task. LC conditions for the separation of authentic alkaloid standards were optimized through the evaluation of various LC parameters in our previous study . The separation of alkaloids on the C18 column was significantly affected by the pH of the mobile phase.…”

Section: Resultsmentioning

confidence: 81%

“…LC conditions for the separation of authentic alkaloid standards were optimized through the evaluation of various LC parameters in our previous study. [25] The separation of alkaloids on the C18 column was significantly affected by the pH of the mobile phase. The HPLC retention behavior of alkaloids on the C18 column decreased significantly at low pH, and increased significantly under neutral pH conditions.…”

Section: Optimum Conditions For Lc/esi-ms/ms Analysismentioning

confidence: 99%

“…[20] LC/MS/MS resolves individual chemical components into separate peaks, even in complex mixtures, thus enhancing the ability to identify minor bioactive compounds. Previous LC/MS/MS studies [21][22][23][24][25] have investigated the fragmentation behaviors of alkaloids, enabling the determination of MS/MS spectral patterns of particular structures.…”

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confidence: 99%

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Identification of alkaloid constituents from Fangchi species using pH control liquid‐liquid extraction and liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry

Sim

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Kim

et al. 2015

Rapid Comm Mass Spectrometry

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Section: Resultsmentioning

confidence: 81%

Section: Optimum Conditions For Lc/esi-ms/ms Analysismentioning

confidence: 99%

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Identification of alkaloid constituents from Fangchi species using pH control liquid‐liquid extraction and liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry

Sim

Soon-Ock

Kim

et al. 2015

Rapid Comm Mass Spectrometry

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“…The simultaneous analysis of the alkaloids with different skeletons present in the Papaver genus is not a simple task. The chromatographic retention behavior of alkaloids on an RP column significantly decreases at low pH, and significantly increases at neutral pH . To optimize the gradient and mass spectrometer conditions, analysis of a mixture of 18 authentic standards (Table ) was performed with the UHPLC–QTOF system.…”

Section: Resultsmentioning

confidence: 99%

Identification and metabolite profiling of alkaloids in aerial parts of Papaver rhoeas by liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry

Lee

et al. 2018

J of Separation Science

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Papaver plants can produce diverse bioactive alkaloids. Papaver rhoeas Linnaeus (common poppy or corn poppy) is an annual flowering medicinal plant used for treating cough, sleep disorder, and as a sedative, pain reliever, and food. It contains various powerful alkaloids like rhoeadine, benzylisoquinoline, and proaporphine. To investigate and identify alkaloids in the aerial parts of P. rhoeas, samples were collected at different growth stages and analyzed using liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry. A liquid chromatography with mass spectrometry method was developed for the identification and metabolite profiling of alkaloids for P. rhoeas by comparing with Papaver somniferum. Eighteen alkaloids involved in benzylisoquinoline alkaloid biosynthesis were used to optimize the liquid chromatography gradient and mass spectrometry conditions. Fifty‐five alkaloids, including protoberberine, benzylisoquinoline, aporphine, benzophenanthridine, and rhoeadine‐type alkaloids, were identified authentically or tentatively by liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry in samples taken during various growth stages. Rhoeadine alkaloids were observed only in P. rhoeas samples, and codeine and morphine were tentatively identified in P. somniferum. The liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry method can be a powerful tool for the identification of diverse metabolites in the genus Papaver. These results may help understand the biosynthesis of alkaloids in P. rhoeas and evaluate the quality of this plant for possible medicinal applications.

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“…UHPLC separation conditions were optimized to facilitate the simultaneous determination of various markers (alkaloids, aristolochic acid I and a lignan) in Fangchi species in a single LC run. The optimized parameters were pH, NH 4 OAc concentration and the composition of the mobile phase, as performed in our previous report [ 15 ]. In addition, the retention of ionizable compounds using reversed-phase HPLC was strongly dependent on the percentage and strength of the organic solvent used in the mobile phase [ 16 , 17 , 18 ].…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS

et al. 2013

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Two bisbenzylisoquinoline alkaloids, two morphine alkaloids, one aporphine alkaloid, syringaresinol and aristolochic acid І were selected as marker compounds and simultaneously analyzed using an ultra-high pressure liquid chromatography-diode array detection (UHPLC-DAD) method. These marker compounds were used for the quality control of Fangchi species of different origins, including Sinomenium acutum, Stephaniatetrandra, Cocculus trilobus and Aristolochia fangchi. A reversed-phase UHPLC-DAD method was developed and validated for the simultaneous quantification of structurally diverse markers in different Fangchi species. In addition, an UHPLC-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method was used for marker identification in Fangchi species, which provided diagnostic MS/MS spectral patterns that were dependent upon the marker structures. The UHPLC-MS/MS data were used to confirm and complement the UHPLC-DAD quality evaluation results. Additionally, magnoflorine and syringaresinol were observed for the first time in S. tetrandra and C. trilobus, respectively. Twenty different Fangchi species samples were analyzed for aristolochic acid I, syringaresinol and the alkaloids using the UHPLC-DAD and MS/MS method. Based on the levels of markers and principal component analysis (PCA), this method allowed for the clear classification of the samples into four different groups representing samples originating from the four species.

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UHPLC Separation of Structurally Diverse Markers in Fangchi Species

Cited by 3 publications

References 18 publications

Identification of alkaloid constituents from Fangchi species using pH control liquid‐liquid extraction and liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry

Identification of alkaloid constituents from Fangchi species using pH control liquid‐liquid extraction and liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry

Identification and metabolite profiling of alkaloids in aerial parts of Papaver rhoeas by liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry

Simultaneous Determination of Structurally Diverse Compounds in Different Fangchi Species by UHPLC-DAD and UHPLC-ESI-MS/MS

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