UHPLC-MS/MS and QRT-PCR profiling of PGP agents and Rhizobium spp. of induced phytohormones for growth promotion in mungbean (var. Co4)

Mogal, Chaitanya; Solanki, Vanrajsinh H.; Kansara, Rohan V.; Jha, Sanjay Kumar; Singh, Susheel; Parekh, Vipulkumar; Rajkumar, B.

doi:10.1016/j.heliyon.2022.e09532

Cited by 6 publications

(5 citation statements)

References 50 publications

(42 reference statements)

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“…In this study, consortia did not perform well for plant growth promotion. Our results are in disagreement with Mogal et al (2022) who reported that consortia of rhizobial bacteria have positive effects on plant growth parameters of mungbean. Previously, Consentino et al (2022) described that inoculation of consortia did not improve growth parameters of lettuce, compared to the inoculation of pure bacterial culture.…”

Section: Resultscontrasting

confidence: 99%

Probing the potential of salinity-tolerant endophytic bacteria to improve the growth of mungbean [Vigna radiata (L.) Wilczek]

Zahra,

Tariq,

Abdullah

et al. 2023

Front. Microbiol.

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Soil salinity is one of the major limiting factors in plant growth regulation. Salinity-tolerant endophytic bacteria (STEB) can be used to alleviate the negative effects of salinity and promote plant growth. In this study, thirteen endophytic bacteria were isolated from mungbean roots and tested for NaCl salt-tolerance up to 4%. Six bacterial isolates, TMB2, TMB3, TMB5, TMB6, TMB7 and TMB9, demonstrated the ability to tolerate salt. Plant growth-promoting properties such as phosphate solubilization, indole-3-acetic acid (IAA) production, nitrogen fixation, zinc solubilization, biofilm formation and hydrolytic enzyme production were tested in vitro under saline conditions. Eight bacterial isolates indicated phosphate solubilization potential ranging from 5.8–17.7 μg mL−1, wherein TMB6 was found most efficient. Ten bacterial isolates exhibited IAA production ranging from 0.3–2.1 μg mL−1, where TMB7 indicated the highest potential. All the bacterial isolates except TMB13 exhibited nitrogenase activity. Three isolates, TMB6, TMB7 and TMB9, were able to solubilize zinc on tris-minimal media. All isolates were capable of forming biofilm except TMB12 and TMB13. Only TMB2, TMB6 and TMB7 exhibited cellulase activity, while TMB2 and TMB7 exhibited pectinase production. Based on in vitro testing, six efficient STEB were selected and subjected to the further studies. 16S rRNA gene sequencing of efficient STEB revealed the maximum similarity between TMB2 and Rhizobium pusense, TMB3 and Agrobacterium leguminum, TMB5 and Achromobacter denitrificans, TMB6 and Pseudomonas extremorientalis, TMB7 and Bradyrhizobium japonicum and TMB9 and Serratia quinivorans. This is the first international report on the existence of A. leguminum, A. denitrificans, P. extremorientalis and S. quinivorans inside the roots of mungbean. Under controlled-conditions, inoculation of P. extremorientalis TMB6, B. japonicum TMB7 and S. quinivorans TMB9 exhibited maximum potential to increase plant growth parameters; specifically plant dry weight was increased by up to 52%, 61% and 45%, respectively. Inoculation of B. japonicum TMB7 displayed the highest potential to increase plant proline, glycine betaine and total soluble proteins contents by 77%, 78% and 64%, respectively, compared to control under saline conditions. It is suggested that the efficient STEB could be used as biofertilizers for mungbean crop productivity under saline conditions after field-testing.

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Section: Resultscontrasting

confidence: 99%

Probing the potential of salinity-tolerant endophytic bacteria to improve the growth of mungbean [Vigna radiata (L.) Wilczek]

Zahra,

Tariq,

Abdullah

et al. 2023

Front. Microbiol.

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“…Thus, in a study by Mogal et al, leguminous plants treated with Rhizobium spp. observed an increase in the content of indoleacetic, indolebutyric, gibberellic, and other acids in the roots [65].…”

Section: Discussionmentioning

confidence: 86%

Microorganisms for Bioremediation of Soils Contaminated with Heavy Metals

et al. 2023

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Heavy-metal contaminants are one of the most relevant problems of contemporary agriculture. High toxicity and the ability to accumulate in soils and crops pose a serious threat to food security. To solve this problem, it is necessary to accelerate the pace of restoration of disturbed agricultural lands. Bioremediation is an effective treatment for agricultural soil pollution. It relies on the ability of microorganisms to remove pollutants. The purpose of this study is to create a consortium based on microorganisms isolated from technogenic sites for further development in the field of soil restoration in agriculture. In the study, promising strains that can remove heavy metals from experimental media were selected: Pantoea sp., Achromobacter denitrificans, Klebsiella oxytoca, Rhizobium radiobacter, and Pseudomonas fluorescens. On their basis, consortiums were compiled, which were investigated for the ability to remove heavy metals from nutrient media, as well as to produce phytohormones. The most effective was Consortium D, which included Achromobacter denitrificans, Klebsiella oxytoca, and Rhizobium radiobacter in a ratio of 1:1:2, respectively. The ability of this consortium to produce indole-3-acetic acid and indole-3-butyric acid was 18.03 μg/L and 2.02 μg/L, respectively; the absorption capacity for heavy metals from the experimental media was Cd (56.39 mg/L), Hg (58.03 mg/L), As (61.17 mg/L), Pb (91.13 mg/L), and Ni (98.22 mg/L). Consortium D has also been found to be effective in conditions of mixed heavy-metal contamination. Due to the fact that the further use of the consortium will be focused on the soil of agricultural land cleanup, its ability to intensify the process of phytoremediation has been studied. The combined use of Trifolium pratense L. and the developed consortium ensured the removal of about 32% Pb, 15% As, 13% Hg, 31% Ni, and 25% Cd from the soil. Further research will be aimed at developing a biological product to improve the efficiency of remediation of lands withdrawn from agricultural use.

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“…SA is also an important signaling molecule for triggering responses to a myriad of abiotic stresses, including cold, heat, drought, ultraviolet, heavy metals, and so on, and participates in developmental signaling pathways that control and regulate many physiological and biochemical processes in normal plant growth, development, flowering, ripening, and aging [8,[27][28][29]53,54,58,59,72,91,96].…”

Section: Interaction Of Sa With Other Molecules In Plant Defense Resp...mentioning

confidence: 99%

“…It is generally held that SA plays vital roles in plant stress tolerance. SA's main roles in plant tolerance to stresses can be summarized as following: the activation of plant defense mechanisms [2,[11][12][13][14], the improvement of photosynthesis [15][16][17][18][19][20][21][22], the regulation of plant normal growth and development [8,19,[23][24][25][26][27][28][29][30], and the enhancement of plant tolerance to drought [9,11,[31][32][33][34][35][36], cold [37][38][39], heat [36,[39][40][41][42], salt [43][44][45][46][47][48], and heavy metal stresses [49][50][51]…”

Section: Introductionmentioning

confidence: 99%

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Advances in Roles of Salicylic Acid in Plant Tolerance Responses to Biotic and Abiotic Stresses

Song,

Shao,

Zheng

et al. 2023

Plants

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A multitude of biotic and abiotic stress factors do harm to plants by bringing about diseases and inhibiting normal growth and development. As a pivotal signaling molecule, salicylic acid (SA) plays crucial roles in plant tolerance responses to both biotic and abiotic stresses, thereby maintaining plant normal growth and improving yields under stress. In view of this, this paper mainly discusses the role of SA in both biotic and abiotic stresses of plants. SA regulates the expression of genes involved in defense signaling pathways, thus enhancing plant immunity. In addition, SA mitigates the negative effects of abiotic stresses, and acts as a signaling molecule to induce the expression of stress-responsive genes and the synthesis of stress-related proteins. In addition, SA also improves certain yield-related photosynthetic indexes, thereby enhancing crop yield under stress. On the other hand, SA acts with other signaling molecules, such as jasmonic acid (JA), auxin, ethylene (ETH), and so on, in regulating plant growth and improving tolerance under stress. This paper reviews recent advances in SA’s roles in plant stress tolerance, so as to provide theoretical references for further studies concerning the decryption of molecular mechanisms for SA’s roles and the improvement of crop management under stress.

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UHPLC-MS/MS and QRT-PCR profiling of PGP agents and Rhizobium spp. of induced phytohormones for growth promotion in mungbean (var. Co4)

Cited by 6 publications

References 50 publications

Probing the potential of salinity-tolerant endophytic bacteria to improve the growth of mungbean [Vigna radiata (L.) Wilczek]

Probing the potential of salinity-tolerant endophytic bacteria to improve the growth of mungbean [Vigna radiata (L.) Wilczek]

Microorganisms for Bioremediation of Soils Contaminated with Heavy Metals

Advances in Roles of Salicylic Acid in Plant Tolerance Responses to Biotic and Abiotic Stresses

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