UHPLC-MS Metabolome Fingerprinting: The Isolation of Main Compounds and Antioxidant Activity of the Andean Species Tetraglochin ameghinoi (Speg.) Speg.

Abstract

The seriated extracts of petroleum ether (PE-E), dichloromethane (DCM-E) and methanol extracts (MeOH-E) from the aerial parts of the native South American plant Tetraglochin ameghinoi (Rosaceae), were evaluated regarding their antioxidant and antibacterial activities. The antioxidant properties were evaluated by free radical scavenging methods (DPPH and TEAC), ferric-reducing antioxidant power (FRAP) and lipoperoxidation in erythrocytes (LP), while the antibacterial activity was performed against Gram-positive and Gram-negative bacteria according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The chemical and biological analyses of this plant are very important since this bush is currently used in traditional medicine as a cholagogue and digestive. The polar MeOH-E showed the highest antioxidant activities (17.70 µg/mL in the DPPH assay, 381.43 ± 22.38 mM TE/g extract in the FRAP assay, 387.76 ± 91.93 mg TE/g extract in the TEAC assay and 93.23 + 6.77% in the LP assay) and it was selected for chromatographic isolation of its components. These components were found to be four acetophenones, including the new phloracetophenone glucoside: 4′,6′,-dihydroxy-2′-O-(6″-acetyl)-β-d-glucopyranosylacetophenone or IUPAC name: (6-(2-acetyl-3,5-dihydroxyphenoxy)-3,4,5-trihydroxytetrahydro-2H-pyran-2-yl)methyl acetate, whose structure was elucidated by NMR and MS methods. In addition, twenty-six compounds, including five of these acetophenone derivatives, two sugars, six flavonoids, eleven phenolic acids and two triterpenes, were identified based on UHPLC-OT-MS and PDA analysis on the MeOH-E. The results support the medicinal use of the plant.

UHPLC-HESI-OT-MS-MS Biomolecules Profiling, Antioxidant and Antibacterial Activity of the “Orange-Yellow Resin” from Zuccagnia punctata Cav.

“…The orange-yellow resin from Z.punctata (ZpRe) displayed a stronger DPPH scavenging activity with an EIC 50 25.72 µg/mL, as well as an outstanding inhibition of lipid peroxidation in erythrocytes (70% percent at 100 µg ZpRe/mL), this was comparable to the value shown by the reference compound catechin (74% at 100µg/mL) ( Table 2) and to the values shown recently by others Andean species [14,15,18]. Phenolic antioxidant compounds acting as free radical scavengers can delay or inhibit lipid oxidation processes.…”

“…Regarding guibourtinidol (14), recently, the hepatoprotective activity and powerful antioxidant properties of Cassia abbreviata root extract, rich in (epi)-catechin, (epi)-afzelechin, (epi)-guibourtinidol, and (ent)-cassiaflavan monomers as well as their dimers and trimers has been reported [66].…”

Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

“…Standards and extracts dissolved in methanol were kept at 10 °C in the auto sampler. MS parameters: The HESI II and other parameters for the Q-orbitrap instrument were optimized also as previously reported [65].…”

Metabolomic Analysis, Fast Isolation of Phenolic Compounds, and Evaluation of Biological Activities of the Bark From Weinmannia trichosperma Cav. (Cunoniaceae)

“…Ultra-high-performance chromatography (UHPLC) coupled to mass spectrometer (MS) hyphenated devices nowadays offer higher sample throughput with more accurate information per sample. In the case of orbitrap technology (OT), it offers the fast and reliable untargeted information of several different metabolites, such as phospholipids, glycans, phenolic compounds, peptides, pesticides, toxins, and other small bioactive molecules (Simirgiotis et al, 2016;Luna et al, 2018;Deng et al, 2019, Yang et al, 2018a. To our knowledge, there are no reports on chemical characterization, antioxidant, antiinflammatory, or enzyme inhibitory capacities of the phenolic-enriched bark obtained from W. trichosperma.…”