uFlow: software for rational engineering of secondary flows in inertial microfluidic devices

Stoecklein, Daniel; Owsley, Keegan; Wu, Chueh‐Yu; Carlo, Dino Di; Ganapathysubramanian, Baskar

doi:10.1007/s10404-018-2093-x

Cited by 11 publications

(14 citation statements)

References 27 publications

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“…S8. We attribute differences between the experimental "UCLA" flow images and those predicted by FlowSculpt to errors in fabrication, which we verify using measured pillar diameters with the uFlow software's interpolative model 19 (see ESI † Fig. S7), and diffusion of the fluorescent dye as it traverses the length of the microchannel.…”

Section: Flow Shape Demonstrationmentioning

confidence: 71%

“…1(b)) to be rapidly concatenated for real-time flow simulation, as demonstrated in the freely available software "uFlow". 18,19 uFlow is a powerful visualization tool for manually exploring inertial flow sculpting, allowing users to rapidly build intuition with the complex design space. However, using uFlow to solve the inverse problem in flow sculptingdesigning a flow sculpting device that produces a target fluid flow shapeis a monumental task given the enormous combinatorial difficulty, which is compounded by the nonlinear physics of flow sculpting.…”

Section: Introductionmentioning

confidence: 99%

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FlowSculpt: software for efficient design of inertial flow sculpting devices

et al. 2019

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Section: Flow Shape Demonstrationmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

FlowSculpt: software for efficient design of inertial flow sculpting devices

et al. 2019

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“…The cross-sectional structure can be controlled by tuning the ratio of volumetric flow rates of prepolymer solutions and the sequence of pillars along the channel length, which deform the prepolymer streams in a controlled manner. We previously developed open source software, FlowSculpt ( 32 ) and uFlow ( 33 ), to rapidly simulate and design the cross-sectional pattern of a co-flow quantitatively and apply it to simulate the locations of the PPGDA and PEGDA streams here ( Fig. 3B ).…”

Section: Resultsmentioning

confidence: 99%

“…We designed the DCPs using custom software built in the lab and open to the public, called uFlow (33). uFlow enables rapid computation of a 3D particle shape formed from the intersection of an extrusion of the flow stream cross-sectional shape and an extrusion of an orthogonal 2D optical mask shape.…”

Section: Microfluidic Channel Designmentioning

confidence: 99%

Monodisperse drops templated by 3D-structured microparticles

Ouyang

Wang

et al. 2020

Sci. Adv.

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The ability to create uniform subnanoliter compartments using microfluidic control has enabled new approaches for analysis of single cells and molecules. However, specialized instruments or expertise has been required, slowing the adoption of these cutting-edge applications. Here, we show that three dimensional–structured microparticles with sculpted surface chemistries template uniformly sized aqueous drops when simply mixed with two immiscible fluid phases. In contrast to traditional emulsions, particle-templated drops of a controlled volume occupy a minimum in the interfacial energy of the system, such that a stable monodisperse state results with simple and reproducible formation conditions. We describe techniques to manufacture microscale drop-carrier particles and show that emulsions created with these particles prevent molecular exchange, concentrating reactions within the drops, laying a foundation for sensitive compartmentalized molecular and cell-based assays with minimal instrumentation.

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“…The numerical simulation revealed that the separation of deformable cells can be achieved by placing a single pillar with a semi-circular cross-section at the flow centerline [36]. Flow sculpting in microchannels with a sequence of pillars placed at different transverse positions have been extensively studied [17, 37–42], but these systems were not shown to be effective for cell-to-cell separation [17, 38, 39]. In particular, complex flow geometries with flow streams moved and split, solution exchange, and separation of embedded identical particles from the solution were created in pillared channels [17].…”

Section: Introductionmentioning

confidence: 99%

Modeling of Deformable Cell Separation in a Microchannel with Sequenced Pillars

Hymel

Fujioka

Khismatullin

2021

Preprint

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Embedded pillar microstructures are an efficient approach for controlling and sculpting shear flow in a microchannel but have not yet demonstrated to be effective for deformability-based cell separation and sorting. Although simple pillar configurations (lattice, line sequence) worked well for size-based separation of rigid particles, they had a low separation efficiency for circulating cells. The objective of this study was to optimize sequenced microstructures for the separation of deformable cells. This was achieved by numerical analysis of pairwise cell migration in a microchannel with multiple pillars, which size, longitudinal spacing, and lateral location, as well as the cell elasticity and size, varied. This study revealed two basic pillar configurations optimized for deformability-based separation: 1) 'duplet' that consists of two closely spaced pillars positioned far below the centerline and above the centerline halfway to the wall; and 2) 'triplet' composed of three widely-spaced pillars located below, above and at the centerline, respectively. The duplet configuration is well suited for deformable cell separation in short channels, while the triplet or a combination of duplets and triplets provides even better separation in long channels. These optimized pillar microstructures can dramatically improve microfluidic methods for sorting and isolation of blood and rare circulating tumor cells.

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uFlow: software for rational engineering of secondary flows in inertial microfluidic devices

Cited by 11 publications

References 27 publications

FlowSculpt: software for efficient design of inertial flow sculpting devices

FlowSculpt: software for efficient design of inertial flow sculpting devices

Monodisperse drops templated by 3D-structured microparticles

Modeling of Deformable Cell Separation in a Microchannel with Sequenced Pillars

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