UFLC/MS-IT-TOF guided isolation of anti-HBV active chlorogenic acid analogues from Artemisia capillaris as a traditional Chinese herb for the treatment of hepatitis

Zhao, Yong; Geng, Chang‐An; Ma, Yun‐Bao; Huang, Xiaoyan; Chen, Hao; Cao, Tuan‐Wu; He, Kun; Wang, Hao; Zhang, Xuemei

doi:10.1016/j.jep.2014.08.043

Cited by 62 publications

(35 citation statements)

References 26 publications

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“…37,41) A. capillaris has been widely used to treat various hepatic disorders in traditional Oriental medicine. 42,43) Some species are used to stabilize sand in desert or semidesert areas or as herbage for feeding livestock.…”

Section: Discussionmentioning

confidence: 99%

DNA Barcode for Identifying Folium Artemisiae Argyi from Counterfeits

Quanxi

Chen

et al. 2016

Biological & Pharmaceutical Bulletin

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Folium Artemisiae Argyi is an important herb in traditional Chinese medicine. It is commonly used in moxibustion, medicine, etc. However, identifying Artemisia argyi is difficult because this herb exhibits similar morphological characteristics to closely related species and counterfeits. To verify the applicability of DNA barcoding, ITS2 and psbA-trnH were used to identify A. argyi from 15 closely related species and counterfeits. Results indicated that total DNA was easily extracted from all the samples and that both ITS2 and psbA-trnH fragments can be easily amplified. ITS2 was a more ideal barcode than psbA-trnH and ITS2 psbA-trnH to identify A. argyi from closely related species and counterfeits on the basis of sequence character, genetic distance, and tree methods. The sequence length was 225 bp for the 56 ITS2 sequences of A. argyi, and no variable site was detected. For the ITS2 sequences, A. capillaris, A. anomala, A. annua, A. igniaria, A. maximowicziana, A. princeps, Dendranthema vestitum, and D. indicum had single nucleotide polymorphisms (SNPs). The intraspecific Kimura 2-Parameter distance was zero, which is lower than the minimum interspecific distance (0.005). A. argyi, the closely related species, and counterfeits, except for Artemisia maximowicziana and Artemisia sieversiana, were separated into pairs of divergent clusters by using the neighbor joining, maximum parsimony, and maximum likelihood tree methods. Thus, the ITS2 sequence was an ideal barcode to identify A. argyi from closely related species and counterfeits to ensure the safe use of this plant. Key words Folium Artemisiae Argyi; DNA barcoding; ITS2; psbA-trnHFolium Artemisiae Argyi, the dried leaves of Artemisia argyi Levl. et Van, is an important herb in traditional Chinese medicine and is commonly used in moxibustion, medicine, food, or medicinal bath.1-4) A. argyi is widely distributed in Korea, Mongolia, Japan, and the Russia Far East.1,5,6) Folium Artemisiae Argyi was first recorded in "Shi Jing" near 1100 BC and in many clinical and medicine literature, such as "Wu Shi Er Bing Fang," "Ben Cao Gang Mu," and "Jin Gui Yao Lve." 3,7) In traditional Chinese medicine, Folium Artemisiae Argyi was considered to have bitter, pungent, and warm properties and associated with the liver, spleen, and kidney meridians.8) Folium Artemisiae Argyi prevents and cures gynecological, respiratory, and dermatological diseases, such as menstruation-related symptoms, infertility, dysmenorrhea, inflammation, hemostasis, tuberculosis, asthma, and eczema. 9-11)Folium Artemisiae Argyi contains complex ingredients, including essential oil, polysaccharide, flavonoid, tannin, sterols, and triterpenes. [12][13][14][15][16] Unfortunately, identifying A. argyi is difficult because this plant exhibits similar morphological characteristics to closely related species and counterfeits, including congeneric plants Artemisia lavandulifolia, Artemisia anomala, Artemisia princeps, Artemisia indica, Artemisia stolonifera, Dendranthema vestitum, and Dendranthema indicum.1-...

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Section: Discussionmentioning

confidence: 99%

DNA Barcode for Identifying Folium Artemisiae Argyi from Counterfeits

Quanxi

Chen

et al. 2016

Biological & Pharmaceutical Bulletin

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“…CA was demonstrated to counteract liver injury induced by hepatotoxic compounds or ischemia/reperfusion . Artemisia capillaris (Yin‐Chen) is another commonly used traditional herb rich in CA . CA has been shown to alleviate liver injury induced by bile duct ligation (BDL) in rats and to decrease liver fibrosis through decreased expression of collagen I and III, transforming growth factors, and vascular epithelial growth factor .…”

Section: Introductionmentioning

confidence: 99%

Modulation of transport and metabolism of bile acids and bilirubin by chlorogenic acid against hepatotoxicity and cholestasis in bile duct ligation rats: involvement of SIRT1‐mediated deacetylation of FXR and PGC‐1α

Zhu

Wang

Cao

et al. 2018

J Hepato Biliary Pancreat

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“…Findings from our study confirm what other studies have found with the antiviral activities of the isolated compounds from this species including the kaempferol and its derivative; kaempferol 8-methyl ether as well as the quercetin derivative; quercetin 3-methyl ether. 35 Other compounds breynioside A, asperphenamate, robustaside A (E)-p-coumaric acid, cristatin A, blumenol A and lygodinolide were also identified in the plant leaves.…”

Section: Discussionmentioning

confidence: 96%

“…46 It has been documented that a great amount of traditional knowledge about the uses of medicinal plant species is still carried and orally transmitted by indigenous peoples. 35 Many workers have therefore chosen the ethnobotanical approach as a means of focusing on plants with pharmacologically active agents of potential therapeutic interest. 36 The documentation of traditional knowledge especially on the medicinal uses of plants has provided many important drugs of modern day.…”

Section: Discussionmentioning

confidence: 99%

“…36 The documentation of traditional knowledge especially on the medicinal uses of plants has provided many important drugs of modern day. 35,36 This principle has been shown to work efficiently in screening for antivirals, where a high percentage of positive results have been obtained. 27 In many parts of the world, ethnoveterinary medicine is frequently used to treat and to control animal diseases in livestock and poultry by the breeders because it is easily accessible compared to conventional drugs, easy to prepare and administer, and cost very little or nothing at all.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Immune Boosting Properties and Combating of Multiple Respiratory Viral Infections by fifteen Euphorbiaceae Plant Extracts

Abd‐Alla¹,

Sweelam²,

El‐Kashak³

et al. 2019

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Objective: Fifteen Euphorbiaceae plant extracts were evaluated for their antiviral and immune boosting properties. Methods: The maximum non-toxic concentration of each extract in specific pathogen-free embryonated chicken eggs was estimated and their antiviral efficacy was determined in terms of reduction in viral titers, measured by the hemagglutination inhibition (HI) assay. The antiviral efficacy was compared with ribavirin. The active extract was further subjected to estimation of total polyphenolic and flavonoid contents. Also, GC/ MS analysis was used to analyze its unsaponifiable and saponifiable matters. Results: The cytotoxic concentration fifty (CC 50 ) of Breynia distacha recorded the value of >200 and >300 for Newcastle disease virus (NDV) and avian influenza virus (AIV) H5N1, respectively. This extract showed antiviral inhibition concentration (IC 50 ) values of ≤ 2 and ≤ 3, respectively and antiviral therapeutic index of 100% compared with ribavirin as standard. The serum of the vaccinated chicks treated with B. distacha has recorded the highest HI titers (7.1 and 8.3 log2) at the day of 28 post vaccination with high protection percentage (100 and 90%) post NDV and AIV H5N1 challenge, respectively. Also, a high HI titer (6.9 log2) was recorded in serum of the vaccinated chicks treated with Aleurites moluccana, Jatropha curcas, Ricinus communis, Putranjiva roxburghii with protection percentage of 95-100% post-NDV challenge. GC/MS analysis allowed identification of 91.10% of the total unsaponifiable matter, (1-heptatriacotanol represented the major component, 24.61%) and 82.51% of the fatty acid methyl esters content (methyl palmitate represented the major component, 26.13 %). Conclusion: This study highlighted the potential of many Euphorbiaceae plants especially B. distacha, to be new candidate of plant-based prototypes that carry antiviral and immunogenic features against respiratory viral infections.has the potential of being more successful in its steps than screening of pure compounds isolated from the products. 8 The spurge family, Euphorbiaceae occupied a special place in the realm of plant families and is scientifically reported for its antiviral, antimicrobial, anticancer, cytotoxic, and antitumor activities. 9 In this respect, the present work aims to search for the use of plants of the Euphorbiaceae family to combat emerging infectious poultry diseases. Fifteen species belong to this family, are widely distributed and easily available throughout various geographical locations in Egypt, 10 were selected and subjected to evaluate their potential anti-viral activities against respiratory tract infection pathogens in specific pathogen-free (SPF) chicken embryos and evaluation of immune boosting properties of these substances in SPF chicks. These plant species are well known to have their traditional claims for cure and/or reduce symptoms of various disorders including infectious diseases of animal and human among folk cultures.

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UFLC/MS-IT-TOF guided isolation of anti-HBV active chlorogenic acid analogues from Artemisia capillaris as a traditional Chinese herb for the treatment of hepatitis

Cited by 62 publications

References 26 publications

DNA Barcode for Identifying Folium Artemisiae Argyi from Counterfeits

DNA Barcode for Identifying Folium Artemisiae Argyi from Counterfeits

Modulation of transport and metabolism of bile acids and bilirubin by chlorogenic acid against hepatotoxicity and cholestasis in bile duct ligation rats: involvement of SIRT1‐mediated deacetylation of FXR and PGC‐1α

Evaluation of Immune Boosting Properties and Combating of Multiple Respiratory Viral Infections by fifteen Euphorbiaceae Plant Extracts

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