Ubiquitous mitochondrial creatine kinase is overexpressed in the conditioned medium and the extract of LNCaP lineaged androgen independent cell lines and facilitates prostate cancer progression

Pang, Bo; Zhang, Hui; Wang, Jian; Chen, Wen Zheng; Li, Shan Hu; Shi, Qing Guo; Liang, Rei Xia; Xie, Bang Xiang; Wu, Rui; Qian, Xiao Long; Lan, Yu; Li, Qi; Huang, Chiu-Jung; Zhou, Jian

doi:10.1002/pros.20969

Cited by 22 publications

(18 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Collection, assay, concentration, and purification of proteins from CM were performed as in reference [25]. Precipitated protein samples (80 ug) were loaded for 2-DE protein separation using 7 cm ReadyStrip IPG Strips (pH 3–10, linear) in strict accordance with the product manual 2-D Electrophoresis for Proteomics (Bio-Rad), Each experiment was repeated independently three times.…”

Section: Methodsmentioning

confidence: 99%

“…Extracellular proteins in CM and holoproteins in CL were harvested and assayed as described in reference [25]. Western blot analyses were also performed as described, except we used goat anti-human SPON2 (R&D Systems), goat anti-human PSA, and rabbit anti-human β-actin (Santa Cruz Biotechnology, Inc.) antibodies [25].…”

Section: Methodsmentioning

confidence: 99%

“…Western blot analyses were also performed as described, except we used goat anti-human SPON2 (R&D Systems), goat anti-human PSA, and rabbit anti-human β-actin (Santa Cruz Biotechnology, Inc.) antibodies [25].…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Spondin-2 (SPON2), a More Prostate-Cancer-Specific Diagnostic Biomarker

Qian

Pang³

et al. 2012

PLoS ONE

Self Cite

View full text Add to dashboard Cite

BackgroundProstate-specific antigen (PSA) screening, although common, has recently been called into question. To find prostate cancer (PCa) diagnostic biomarkers that can make up for the defects of PSA, we compared the secretomes of several benign and PCa cell lines, selected candidate molecules, and then confirmed their clinical value.Methodology/Principal FindingsWe first identified extracellular proteins by two-dimensional gel electrophoresis (2-DE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification. We then validated the secreted proteins on a cellular level, and finally determined whether they could be used as PCa diagnostic biomarkers using prostate tissue and serum specimens of Chinese volunteers by immunohistostaining and sandwich ELISA. We obtained credible extracellular protein 2-DE graphs of prostate cell lines. The 5 spots that showed superior repeatability were selected for LC-MS/MS analysis, which identified seven candidate molecules. One of the candidate molecules, spondin-2 (SPON2), was only expressed in the conditioned media (CM) of androgen receptor (AR) positive PCa cell lines. Using tissue microarray by immunohistostaining, we found SPON2 to be over-expressed in PCa. SPON2 staining was more intense in Gleason score sum 7–8 and in PCa patients with metastasis. By receiver operator characteristic (ROC) curve analysis, we found that the serum SPON2 level was elevated in PCa patients, showing sensitivity and specificity suitable for diagnostic use. We also found that SPON2 could be used to identify PCa patients with serum PSA levels no higher than 10 ng/ml from healthy elderly men.Conclusion/SignificanceSPON2 is a new serum and histological diagnostic biomarker for PCa. It can avoid some of the problems of PSA testing and was here found to offer relatively high sensitivity and specificity relative to PSA.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Spondin-2 (SPON2), a More Prostate-Cancer-Specific Diagnostic Biomarker

Qian

Pang³

et al. 2012

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…Western blot analysis was performed as described previously (6). Rabbit anti-SGEF (Sigma, St. Louis, MO, USA), mouse actin antibody (Santa Cruz Biotechnology, Inc., Heidelberg, Germany), rabbit anti-AKT and rabbit anti-SGEF is overexpressed in prostate cancer and contributes to prostate cancer progression AKT/phosphor Ser473 (Cell Signaling Technology, Inc., Danvers, MA, USA) were used as primary antibodies.…”

Section: Methodsmentioning

confidence: 99%

“…Immunohistochemistry was performed as described previously using TMA (Chaoying Biotechnology Co., Xi'an, China) including 75 prostate specimens (10 benign prostate tissues, 19 BPH tissues and 46 tumor tissues) (6). SGEF expression was scored blindly by staining intensity in each biopsy core as being weak, moderate or strong.…”

Section: Rt-pcrmentioning

confidence: 99%

SGEF is overexpressed in prostate cancer and contributes to prostate cancer progression

Wang¹,

Wu²,

Lan³

et al. 2012

Oncology Reports

Self Cite

View full text Add to dashboard Cite

Abstract. The purpose of this study was to investigate the potential roles of the SH3-containing guanine nucleotide exchange factor (SGEF) in human prostate cancer. Experimental data showed that SGEF was overexpressed in human prostate cancer cells and specimens. The reduction of SGEF expression through an SGEF-targeting siRNA in androgen-independent C4-2 and C4-2B cells suppressed both anchorage-dependent and anchorage-independent growth. In addition, the androgen receptor (AR) antagonist bicalutamide further strengthened this inhibitory effect due to the suppression of the elevated AR transactivation after knockdown of SGEF. Collectively, our results provide the first demonstration that SGEF is a novel promoter of human prostate cancer progression and development.

show abstract

Mitochondrial Proteolipid Complexes of Creatine Kinase

Schlattner

Kay

Tokarska‐Schlattner

2018

Subcellular Biochemistry

View full text Add to dashboard Cite

Isoforms of creatine kinase (CK) generate and use phosphocreatine, a concentrated and highly diffusible cellular "high energy" intermediate, for the main purpose of energy buffering and transfer in order to maintain cellular energy homeostasis. The mitochondrial CK isoform (mtCK) localizes to the mitochondrial intermembrane and cristae space, where it assembles into peripherally membrane-bound, large cuboidal homooctamers. These are part of proteolipid complexes wherein mtCK directly interacts with cardiolipin and other anionic phospholipids, as well as with the VDAC channel in the outer membrane. This leads to a stabilization and cross-linking of inner and outer mitochondrial membrane, forming so-called contact sites. Also the adenine nucleotide translocator of the inner membrane can be recruited into these proteolipid complexes, probably mediated by cardiolipin. The complexes have functions mainly in energy transfer to the cytosol and stimulation of oxidative phosphorylation, but also in restraining formation of reactive oxygen species and apoptosis. In vitro evidence indicates a putative role of mtCK in mitochondrial phospholipid distribution, and most recently a role in thermogenesis has been proposed. This review summarizes the essential structural and functional data of these mtCK complexes and describes in more detail the more recent advances in phospholipid interaction, thermogenesis, cancer and evolution of mtCK.

show abstract

Ubiquitous mitochondrial creatine kinase is overexpressed in the conditioned medium and the extract of LNCaP lineaged androgen independent cell lines and facilitates prostate cancer progression

Abstract: We showed that uMtCK could be easily detected in CM of LNCaP lineaged AIPC cells. Exogenous uMtCK in LNCaP cells surprisingly contributed to overproduction of ROS, activation of Akt signaling pathway and more aggressive phenotypes including androgen independence development.

Cited by 22 publications

References 35 publications

Spondin-2 (SPON2), a More Prostate-Cancer-Specific Diagnostic Biomarker

Spondin-2 (SPON2), a More Prostate-Cancer-Specific Diagnostic Biomarker

SGEF is overexpressed in prostate cancer and contributes to prostate cancer progression

Mitochondrial Proteolipid Complexes of Creatine Kinase

Contact Info

Product

Resources

About