Ubiquitin immunoreactivity shows several proteins varying with development and sporulation in the basidiomycete <i>Coprinus cinereus</i>

Kanda, Tadahito; Tanaka, Nobuharu; Takemaru, Tsuneo

doi:10.1139/o90-150

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…Therefore, degradation of unnecessary proteins through the ubiquitin–proteasome pathway is presumably linked with fruiting body development in basidiomycetes. Kanda et al . (1990a, b) reported on ubiquitin‐mediated protein degradation during fruiting in Coprinus cinereus . Cytochrome P450 : Two cDNAs (82f and 810i) are presumed to encode proteins homologous to cytochrome P450.…”

Section: Resultsmentioning

confidence: 99%

Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomyceteFlammulina velutipesby fluorescence differential display

Yamada

Sakuraba

Shibata

et al. 2006

FEMS Microbiology Letters

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Using fluorescence differential display, cDNAs specifically expressed at the primordial stage of fruiting body development were isolated from the basidiomycete, Flammulina velutipes. Seventy-five cDNAs were sequenced and compared with the amino-acid sequences of proteins in the database by BLASTX search. Significant similarity was found for 29 cDNAs coding for proteins with known function, GTP-binding protein, growth factor, ubiquitin-proteasome, cytochrome P450 and hydrophobin, all of which would be associated with fruiting body development. Seventeen cDNAs were not similar to proteins in the database and may represent unique genes that play specific roles in the process of fruiting in F. velutipes.

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Section: Resultsmentioning

confidence: 99%

Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomyceteFlammulina velutipesby fluorescence differential display

Yamada

Sakuraba

Shibata

et al. 2006

FEMS Microbiology Letters

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“…Studying cytology and the genetics of development in C. cinereus will also reveal how alike and how different a basidiomycete is compared to other eukaryotes and, in particular, to the better-understood ascomycetous fungi including the yeasts S. cerevisiae and S. pombe and the filamentous fungi Aspergillus nidulans and N. crassa. Few genes (e.g., cgl1, cgl2, eln2, ich1, and pcc1) and some differentially expressed proteins of undefined function (217,218) are to date known that are specific to the C. cinereus fruiting body or necessary for its formation. There have been surprising discoveries in this fungus within the small number of products so far identified from cloned genes, such as the galectins, only known in animals till now, and the putative cyclopropane fatty acid synthetase, believed so far to be a bacterium-specific enzyme.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Life History and Developmental Processes in the BasidiomyceteCoprinus cinereus

Kües

2000

Microbiol Mol Biol Rev

432

336

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SUMMARY Coprinus cinereus has two main types of mycelia, the asexual monokaryon and the sexual dikaryon, formed by fusion of compatible monokaryons. Syngamy (plasmogamy) and karyogamy are spatially and temporally separated, which is typical for basidiomycetous fungi. This property of the dikaryon enables an easy exchange of nuclear partners in further dikaryotic-monokaryotic and dikaryotic-dikaryotic mycelial fusions. Fruiting bodies normally develop on the dikaryon, and the cytological process of fruiting-body development has been described in its principles. Within the specialized basidia, present within the gills of the fruiting bodies, karyogamy occurs in a synchronized manner. It is directly followed by meiosis and by the production of the meiotic basidiospores. The synchrony of karyogamy and meiosis has made the fungus a classical object to study meiotic cytology and recombination. Several genes involved in these processes have been identified. Both monokaryons and dikaryons can form multicellular resting bodies (sclerotia) and different types of mitotic spores, the small uninucleate aerial oidia, and, within submerged mycelium, the large thick-walled chlamydospores. The decision about whether a structure will be formed is made on the basis of environmental signals (light, temperature, humidity, and nutrients). Of the intrinsic factors that control development, the products of the two mating type loci are most important. Mutant complementation and PCR approaches identified further genes which possibly link the two mating-type pathways with each other and with nutritional regulation, for example with the cAMP signaling pathway. Among genes specifically expressed within the fruiting body are those for two galectins, β-galactoside binding lectins that probably act in hyphal aggregation. These genes serve as molecular markers to study development in wild-type and mutant strains. The isolation of genes for potential non-DNA methyltransferases, needed for tissue formation within the fruiting body, promises the discovery of new signaling pathways, possibly involving secondary fungal metabolites.

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“…The higher levels of LeUbi mRNA in primordium suggests that L. edodes undergoes rapid ribosomal biogenesis and protein turnover in fruiting. In C. cinereus, the levels of ubiquitin and several ubiquitinrelated proteins increase during primordium formation but decrease during fruit body maturation (Kanda et al 1990). …”

Section: Discussionmentioning

confidence: 99%

Identification by RNA fingerprinting of genes differentially expressed during the development of the basidiomycete Lentinula edodes

Leung¹,

Zhang²,

Xie³

et al. 2000

Mol Gen Genet

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As part of an ongoing project to understand the molecular mechanisms of fruit body development in Lentinula edodes (Shiitake mushroom), RNA fingerprinting by arbitrarily primed PCR (RAP-PCR) was used to identify differentially expressed genes in RNA populations from four stages of L. edodes development vegetative mycelium, primordium, young fruit body and mature fruit body. From 30 RNA fingerprints, we cloned and sequenced 33 RAP fragments after their differential expression patterns had been verified by reverse Northern dot-blot hybridization. Thirteen RAP fragments show high sequence similarity to known gene products which are involved in (1) transport across the plasma membrane (drug efflux pump and sugar transporter); (2) cell cycle control (cyclin B); (3) signal transduction and transcriptional regulation (mitogen-activated protein kinase, Cdc39/Not1, PriA, Jun-D); (4) intracellular molecule trafficking (ubiquitin, plasma membrane proton ATPase, and alpha-adaptin); (5) mitochondrial biogenesis (mitochondrial processing peptidase beta-subunit, mitochondrial glycerol-3-phosphate dehydrogenase); and (6) intermediary metabolism (fructose 1,6 bisphosphatase). The transcript levels for plasma membrane proton ATPase and alpha-adaptin remained constant, whereas the other eleven genes were differentially expressed during L. edodes. development. The expression profiles of the genes suggest that transport across the plasma membrane is important in the mycelial stage. Specific signal transduction and transcriptional controls may play important roles during the initiation of primordia and the formation of young fruiting bodies. When the mushroom matures, expression of genes involved in metabolic pathways becomes prominent. The isolation of these genes indicates their involvement in homobasidiomycete development and suggests new directions for molecular studies on mechanisms of mushroom development.

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Ubiquitin immunoreactivity shows several proteins varying with development and sporulation in the basidiomycete Coprinus cinereus

Cited by 6 publications

References 18 publications

Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomyceteFlammulina velutipesby fluorescence differential display

Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomyceteFlammulina velutipesby fluorescence differential display

Life History and Developmental Processes in the BasidiomyceteCoprinus cinereus

Identification by RNA fingerprinting of genes differentially expressed during the development of the basidiomycete Lentinula edodes

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