Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomycete<i>Flammulina velutipes</i>by fluorescence differential display

Yamada, Masato; Sakuraba, Sou; Shibata, Kou; Taguchi, Goro; Inatomi, Satoshi; Okazaki, Mitsuo; Shimosaka, Makoto

doi:10.1111/j.1574-6968.2005.00023.x

Cited by 28 publications

(25 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also noticed that some genes were commonly expressed at the two stages, such as proteasome regulatory subunit, ribosome protein, sterol dehydrogenase. Proteasome regulatory subunit mainly participates in protein degradation and was reported to be up-regulated in primordium of Flammulina velutipes [73]. Both proteasome regulatory subunit and ribosome proteins were highly expressed from mycelium to fruiting body, which implied that an increase in protein varieties was needed for cell differentiation during development.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome and Proteome Exploration to Provide a Resource for the Study of Agrocybe aegerita

Wang

Huang

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

Background Agrocybe aegerita, the black poplar mushroom, has been highly valued as a functional food for its medicinal and nutritional benefits. Several bioactive extracts from A. aegerita have been found to exhibit antitumor and antioxidant activities. However, limited genetic resources for A. aegerita have hindered exploration of this species.Methodology/Principal FindingsTo facilitate the research on A. aegerita, we established a deep survey of the transcriptome and proteome of this mushroom. We applied high-throughput sequencing technology (Illumina) to sequence A. aegerita transcriptomes from mycelium and fruiting body. The raw clean reads were de novo assembled into a total of 36,134 expressed sequences tags (ESTs) with an average length of 663 bp. These ESTs were annotated and classified according to Gene Ontology (GO), Clusters of Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways. Gene expression profile analysis showed that 18,474 ESTs were differentially expressed, with 10,131 up-regulated in mycelium and 8,343 up-regulated in fruiting body. Putative genes involved in polysaccharide and steroid biosynthesis were identified from A. aegerita transcriptome, and these genes were differentially expressed at the two stages of A. aegerita. Based on one-dimensional gel electrophoresis (1-DGE) coupled with electrospray ionization liquid chromatography tandem MS (LC-ESI-MS/MS), we identified a total of 309 non-redundant proteins. And many metabolic enzymes involved in glycolysis were identified in the protein database.Conclusions/SignificanceThis is the first study on transcriptome and proteome analyses of A. aegerita. The data in this study serve as a resource of A. aegerita transcripts and proteins, and offer clues to the applications of this mushroom in nutrition, pharmacy and industry.

show abstract

Section: Resultsmentioning

confidence: 99%

Transcriptome and Proteome Exploration to Provide a Resource for the Study of Agrocybe aegerita

Wang

Huang

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…So far, cytochrome P450 enzymes have been reported to be involved in producing phytohormones in plants (Winkier and Helentjaris 1995;Feldmann 2001). Furthermore, a variety of developmentally specifi c genes encoding several types of cytochrome P450 enzymes and several proteins involved in membrane transport and endocytosis have been identifi ed in edible mushrooms L. edodes, Pleurotus ostreatus, and F. velutipes (Akiyama et al 2002;Miyazaki et al 2005;Yamada et al 2006;Joong-Ho et al 2007;Lee et al 2007). These results led us to propose another possibility: both Cc.ctg1 (Cc.Ctg1) and ctg1 (CTG1) are involved in production (or regulation of production) or transport of a sort of signal compound.…”

Section: Discussionmentioning

confidence: 96%

The homologue of Lentinula edodes ctg1, a target for CDC5 and its interacting partner CIPB, from Coprinopsis cinerea is involved in fruitingbody morphogenesis of C. cinerea

et al. 2009

View full text Add to dashboard Cite

We have previously isolated a target gene for the Lentinula edodes CDC5 (Le.CDC5) containing a c-Mybtype DNA-binding domain. The gene, termed ctg1, contains a 7-bp Le.CDC5-binding sequence (5′-GCAATCT3′) in its transcribed region downstream of the start codon and 6-bp CIPB-binding sequences in the 5′-upstream and transcribed regions. The expression of ctg1 appeared to be cooperatively regulated by Le.CDC5 and CIPB. Here, we found that the gene expression regulation system of ctg1-CDC5-CIPB is conserved in the basidiomycetous mushroom Coprinopsis cinerea. Similar to L. edodes, the Le.CDC5 homologue (named Cc.Cdc5) bound to a 7-bp sequence, 5′-GCAAGCT-3′, in the transcribed region of the ctg1 homologue (Cc.ctg1) and the CIPB homologue (Cc.CipB) to 6-bp sequences in its 5′-upstream and transcribed regions. To study the biological function of the Cc.ctg1 gene (and also the ctg1 gene) in C. cinerea, we bred the C. cinerea homokaryotic fruiting strains, consistently producing a large amount of the Cc.ctg1 (or ctg1) transcript. Analysis of the phenotype of these strains suggested that the Cc.ctg1 gene (or the ctg1 gene) is mainly involved in the regulation of stipe elongation.

show abstract

“…They include GTP-binding protein, growth factor, ubiquitineproteasome, cytochrome P450 and hydrophobin (Yamada et al 2006). In Lentinula edodes, by cDNA representational difference analysis, 51 and 54 individual cDNAs have been isolated specifically in primordium and mature fruiting body.…”

Section: Introductionmentioning

confidence: 99%

Opposing developmental functions of Agrocybe aegerita galectin (AAL) during mycelia differentiation

et al. 2010

View full text Add to dashboard Cite

Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomyceteFlammulina velutipesby fluorescence differential display

Cited by 28 publications

References 28 publications

Transcriptome and Proteome Exploration to Provide a Resource for the Study of Agrocybe aegerita

Transcriptome and Proteome Exploration to Provide a Resource for the Study of Agrocybe aegerita

The homologue of Lentinula edodes ctg1, a target for CDC5 and its interacting partner CIPB, from Coprinopsis cinerea is involved in fruitingbody morphogenesis of C. cinerea

Opposing developmental functions of Agrocybe aegerita galectin (AAL) during mycelia differentiation

Contact Info

Product

Resources

About