Über ein gebundenes Gibberellin ausPhaseolus coccineus L.

Sembdner, G.; Schneider, G.; Weiland, Jürgen; Schréiber, Klaus

doi:10.1007/bf02151258

Cited by 36 publications

(8 citation statements)

References 11 publications

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“…The presence of neutral bound gilbberellins has been reported by a nutmber of authors (3,10,13,18). Evidence was presented that acidic gibberellins could be recovered from such complexes by treatment with glycolytic (13) and proteolytic (10) enzymes as well as by acid hydrolysis (18). The aqueous fraction was either inctbated with hydrolytic enzymes, or hydrolyzed with acid as specified in table III.…”

Section: Resultsmentioning

confidence: 88%

Preparation of Radioactive Gibberellin A₁ and its Metabolism in Dwarf Peas

Kende

1967

Plant Physiol.

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Summary. G(iberellin A1-3,4-3H was prepared by selective catalytic reduction of gibberellic acid with a mixture of tritium and hydrogen. 3H-GA1 was applied at physiological concentrations to dwarf peas and the metabolism of the horimone was investigated. tH-GA1 was converted to an acidic, biologically active compound. Radioactive but biologically inactive compounds were also found in the neutral fraction and could not be converted to

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Section: Resultsmentioning

confidence: 88%

Preparation of Radioactive Gibberellin A₁ and its Metabolism in Dwarf Peas

Kende

1967

Plant Physiol.

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“…The methyl and dimethyl esters of GA3 have also been reported (10). From other plant sourtlces there is also evidence of neuitral and bound gibberellins with glucoside (19) and possibly other carbohydrate and ninhydrin-positive moieties (31). Both the glucoside and the bound gibberellins of Sembdnier et al were soluble in n-butyl alcohol following ethyl acetate extraction.…”

Section: Discussionmentioning

confidence: 96%

Physiological Effects of Gibberellic Acid. X. The Release of Gibberellin-Like Substances by Germinating Barley Embryos

Cohen

Paleg

1967

Plant Physiol.

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Sum,itmary. Barley embryos, completely free from endospernm, wvere excised from germinating grain at various times and al'lowed to diffuse into an aqueous medium for varying lengths of time. At the end of this time, the embrvos and ambient solutioins were separately extracted. Gibberellin-like activity in the extracts was determined with the barley endosperm bioassay using seed from the same varietv, hiarvest and treatment schedule as was employed for the emibryo diffusion experiments. Gibberellin-like sutbstances were released by embryos thTroughout the 60 hour germination period, though at no time during this period could sufficient activity be extracted from the embryos themiiselves to account for the observed release. Solvent partitionling and chromatography identified at least one major acidic component migrating at an Rf similar to that of GA3.It is concluded that the endogenous gibberellin-like substance(s) originates within the embryo during germination, and that the release of this substance('s) is temporally consistent with, and quantitatively sufficient to account for the in vivo endosperm mobilization response synldroIiie. A gilbberellin-Fke substance is uindotlubtedly the endospernm iinI)ilizinlr hormiionie.In 1890 Brown and Morris (5)

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“…A GA present in large amounts at one developmental stage of a plant need not hold this place in the next. For example, developing seeds of Phaseolus coccineus contain GAl, GAs, GA., GA6, GAs, GAl7, GAI9, and GAzo (see Table 1), but none of them can be demonstrated in the mature seed (19,20) , nor do the two major GA-like substances of Ph. coccineus seedlings seem to be identical with any of them (157,158) .…”

Section: Techniques (Diffusion Extraction Assays )mentioning

confidence: 99%