Über die Umlagerung des Colchicins mit Natriumalkoholat und die Struktur des Ringes C

Fernholz, Hans

doi:10.1002/jlac.19505680106

Cited by 45 publications

(16 citation statements)

References 10 publications

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“…Allocolchicine was synthesized by following a published method (Fernholz, 1950). Purified tubulin was pre-clarified by high-speed centrifugation in TLA120.1 rotor (Beckman Coulter) at 90000 rpm for 10 mins at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Mutations in Human Tubulin Proximal to the Kinesin-Binding Site Alter Dynamic Instability at Microtubule Plus- and Minus-Ends

Pamula

Howes

et al. 2016

Developmental Cell

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The assembly of microtubule-based cellular structures depends on regulated tubulin polymerization and directional transport. Here, we purify and characterize tubulin heterodimers that have human β-tubulin isotype III (TUBB3), and heterodimers with one of two β-tubulin mutations (D417H or R262H). Both point mutations are proximal to the kinesin binding site and have been linked to an ocular motility disorder in humans. Compared to wild-type, microtubules with these mutations have decreased catastrophe frequencies and increased average lifetimes of plus- and minus-end stabilizing caps. Importantly, the D417H mutation does not alter microtubule lattice structure or Mal3 binding to growing filaments. Instead, this mutation reduces the affinity of tubulin for TOG domains and colchicine, suggesting that the distribution of tubulin heterodimer conformations is changed. Together, our findings reveal how residues on the surface of microtubules, distal from the GTP-hydrolysis site and inter-subunit contacts, can alter polymerization dynamics at the plus- and minus-ends of microtubules.

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Section: Methodsmentioning

confidence: 99%

Mutations in Human Tubulin Proximal to the Kinesin-Binding Site Alter Dynamic Instability at Microtubule Plus- and Minus-Ends

Pamula

Howes

et al. 2016

Developmental Cell

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“…Allocolchicine was synthesized by the established method ( Fernholz, 1950 ). Purified tubulin was preclarified by high-speed centrifugation in a TLA120.1 rotor (Beckman Coulter) at 90,000 rpm for 10 min at 4°C.…”

Section: Methodsmentioning

confidence: 99%

The structured core of human β tubulin confers isotype-specific polymerization properties

Pamula

Kapoor

2016

Journal of Cell Biology

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“…Experimental. Allocolchicine was prepared from colchicine according to the method of Fernholz (1950). Colourless prismatic crystals elongated along b from methanol (m.p.…”

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confidence: 99%

Structures of colchicine analogues. I. Allocolchicine

Mackay

Lacey²,

Burden³

1989

Acta Crystallogr C

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Methyl 5-acetylamino-6,7-dihydro-9,10,11-trimethoxy-5H-dibenzo[a,c] cycloheptene-3-carboxylate, C22H25NO6, Mr = 399.4, monoclinic, P2(1), a = 7.968 (1), b = 9.482 (1), c = 15.063 (2) A, beta = 112.95 (1) degrees, V = 1047.9 (2) A3, Z = 2, Dm(flotation) = 1.27 (1), Dx = 1.266 Mg m 3, lambda(Cu K alpha) = 1.5418 A, mu = 0.68 mm 1, F(000) = 424, T = 288 (1) K. Final R = 0.050 for 1765 observed data. The seven-membered ring has the expected boat conformation, and the angle between the normals to the phenyl rings is 48.7 (4) degrees. The relative orientations of the three adjacent methoxy groups and the acetamido substituent are similar to those observed in crystals of colchicine. Intermolecular hydrogen bonds between the N and O atoms of the acetamido groups link the molecules into infinite spirals along the b axis.

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Über die Umlagerung des Colchicins mit Natriumalkoholat und die Struktur des Ringes C

Cited by 45 publications

References 10 publications

Mutations in Human Tubulin Proximal to the Kinesin-Binding Site Alter Dynamic Instability at Microtubule Plus- and Minus-Ends

Mutations in Human Tubulin Proximal to the Kinesin-Binding Site Alter Dynamic Instability at Microtubule Plus- and Minus-Ends

The structured core of human β tubulin confers isotype-specific polymerization properties

Structures of colchicine analogues. I. Allocolchicine

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