Über die quantitative Berücksichtigung der Tyndallabsorption im UV-Absorptionsspektrum von Proteinen

Bayzer, H.; Schauenstein, E.

doi:10.1007/bf01235018

Cited by 5 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1. All the data haVe been corrected for Rayleigh scattering by measuring the scattering as a function of wavelength and extrapolating a double logarithmic plot into the region of consumptive absorption (5,26). It is seen from Fig.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Stability of Ribosomes from Mesophilic and Thermophilic Bacteria

Stenesh

Yang

1967

J Bacteriol

View full text Add to dashboard Cite

Ribosomes were isolated from three mesophilic and three thermophilic strains of Bacillus. The ribosomes consisted of about 55% protein and 45% ribonucleic acid. Average ratios for the absorbance at 260/235 and 260/280 mu were 1.77 and 1.92 for the mesophiles and 1.63 and 1.84 for the thermophiles. Ultracentrifugation revealed mainly components with sedimentation coefficients of about 30, 50, 70, 100, and 120S. All the preparations were shown to contain a ribonuclease which, in the presence of ethylenediaminetetraacetic acid, led to ribosome breakdown as measured by the increase in acid-soluble nucleotides. The stability of the ribosomes from the thermophiles was consistently greater than that of the ribosomes from the mesophiles. After 5 hr at 37 C, the breakdown was about 80% for the ribosomes from the mesophiles and 55 to 70% for those from the thermophiles. At 60 C, the ribosomes from the mesophiles were broken down slightly more and at a faster rate than those from the thermophiles. At temperatures above 60 C, the breakdown was again more pronounced for the ribosomes from the mesophiles.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization and Stability of Ribosomes from Mesophilic and Thermophilic Bacteria

Stenesh

Yang

1967

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…This change is termed the hyperchromic effect and is generally considered to be due to breakage of H-bonds in the RNA (Hall and Doty, 1959). A decrease in the 2ß / 235 ratio also furnishes a very sensitive index of aggregation, since an increase in turbidity would result in more light of shorter wave lengths being scattered (Bayzer and Schauenstein, 1955). Finally, chemical and enzymatic analyses of dialyzable and/or sedimentable components have been useful in evaluating the sequence and quantitative significance of a number of reactions.…”

mentioning

confidence: 99%

Beef Pancreas Ribosomes: Stability^*

Madison¹,

Dickman²

1963

Biochemistry

View full text Add to dashboard Cite

Möglichkeiten und Grenzen der Anwendung der UV‐Spektrometrie in der Fettforschung

Schauenstein

1959

Nahrung

View full text Add to dashboard Cite

show abstract

Über die quantitative Berücksichtigung der Tyndallabsorption im UV-Absorptionsspektrum von Proteinen

Cited by 5 publications

References 5 publications

Characterization and Stability of Ribosomes from Mesophilic and Thermophilic Bacteria

Characterization and Stability of Ribosomes from Mesophilic and Thermophilic Bacteria

Beef Pancreas Ribosomes: Stability^*

Möglichkeiten und Grenzen der Anwendung der UV‐Spektrometrie in der Fettforschung

Contact Info

Product

Resources

About

Über die quantitative Berücksichtigung der Tyndallabsorption im UV-Absorptionsspektrum von Proteinen

Cited by 5 publications

References 5 publications

Characterization and Stability of Ribosomes from Mesophilic and Thermophilic Bacteria

Characterization and Stability of Ribosomes from Mesophilic and Thermophilic Bacteria

Beef Pancreas Ribosomes: Stability*

Möglichkeiten und Grenzen der Anwendung der UV‐Spektrometrie in der Fettforschung

Contact Info

Product

Resources

About

Beef Pancreas Ribosomes: Stability^*