Über den Schwefelstoffwechsel von Microsporum canis*

Ziegler, H.; Reichmann, G.

doi:10.1111/j.1439-0507.1968.tb04458.x

Cited by 19 publications

(1 citation statement)

References 14 publications

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“…During the growth on synthetic media enriched with free L-cystine this substance is utilized not only as a source of sulfur but also of carbon, nitrogen, and energy. Moreover, cystine ist intensively metabolized in media containing more suitable nutrients where utilization of this compound with an unsuitable C:N:S ratio leads to growth inhibition (3,11,12,16,19,20,21). This facts lead to the presumption that some toxic derivatives of cystine may be readily metabolized by dermatophytes and exhibit a significant antifungal activity.…”

Section: Introductionmentioning

confidence: 99%

Antifungal Effects of Selenocystine and its Derivatives on Dermatophytes: Die antimyzetische Wirkung von Selenocystin und seinen Derivaten auf Dermatophyten

Kunert¹

1989

Mycoses

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Antifungal effects of DL-selenocystine and its derivatives (DL-selenolanthionine, DL-Se-sulfoselenocysteine) and of sodium selenite were tested in 14 dermatophytes and 7 species of keratinolytic and non-keratinolytic soil fungi. Minimal inhibitory concentrations of the compounds were measured and compared to those of ketoconazole. Organic selenocompounds behaved similarly. They were on average less inhibitory than ketoconazole but, in some cases, MICs were equal or even lower. The effects of selenite were but small. The action of selenocystine and ketoconazole in concentrations up to 50 pg/ml was found to be mostly fungistatic. Free L-cystine (100 pg/ml) antagonized the antifungal effect of its selenoanalog. Zusammenfassung: Die antimyzetische Wirkung von DL-Selenocystin und seiner Derivate DL-Selenolanthionin und DL-Se-Sulfoselenocystein sowie von Natriumselenit wurde an 14 Dermatophyten und 7 Arten keratinolytischer und nichtkeratinolytischer Bodenpilze bestimmt. Es wurden die minimalen Hemmkonzentrationen dieser Verbindungen gemessen und mit Ketoconazol verglichen. Die organischen Selen-verbindungen verhielten sich ahnlich; sie hemmten durchschnittlich etwas weniger als Ketoconazol, in einigen Fallen waren die MHK jedoch gleich oder geringer. Die Selenit-Wirkung war nur gering. Die Wirkung von Selenocystin und Ketoconazol in Konzentrationen bis zu 50 pg/ml erwies sich meist als fungistatisch. Freies L-Cystin (100 pg/ml) antagonisiert die antimyzetische Wirkung seines Selenanalogs.

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Section: Introductionmentioning

confidence: 99%

Antifungal Effects of Selenocystine and its Derivatives on Dermatophytes: Die antimyzetische Wirkung von Selenocystin und seinen Derivaten auf Dermatophyten

Kunert¹

1989

Mycoses

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Keratin decomposition by dermatophytes. I. Sulfite production as a possible way of substrate denaturation

Kunert

1973

J of Basic Microbiology

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The dermatophyte Nicrosporum gypseum was grown on children's hairs in a mineral solution. During the growth of the fungus the substrate digestion, pH of the medium, and concentrations of soluble decomposition products were determined. I n aerated and stationary cultures the substrate digestion attained was 56% and 37 to 45%. respectively. The decomposition was accompanied by the alkalinization of the medium and by the release of peptidic substances.The average content of sulfur amino acids in soluble decomposition products was always lower than their content in keratin. Sulfhydryl compounds (cysteine) were found in traces only. The final product of the cystine metabolism was the sulfate excreted in high amounts into the medium. Sulfides, thiosulfates, and free sulfite were not present. However, after the treatment of the culture filtrates with cyanide, arsenite or cysteine well determinable amounts of sulfite were liberated. These reactions are typical of S-sulfocysteine.The author presents the hypothesis that the dermatophytes can produce not only sulfate, but also sulfite in the course of keratinolysis. By the latter compound, disulfide bonds of the substrate could be cleaved to cysteine and S-sulfocysteine. The keratin denatured by "sulfitolysis" would then be easily attacked by the proteases of the fungus.

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Metabolism of sulfur‐containing amino acids in the dermatophyte Microsporum gypseum I. Neutral amino acids

Kunert

1985

J. Basic Microbiol.

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The dermatophyte Microsporum gypseum was cultivated on a glucose-arginine medium to which on out of six sulfur-containing amino acids was added (L-cystine, L-djenkolic acid, DL-lanthionine DL-homocystine, L-methionine, or L-methionine-sulfone at a concentration of 5 mM with respect to sulfur content). The addition of these substances did not stimulate the growth and some amino acids (djenkolic acid and particularly methionine and methionine-sulfone) were inhibitory. All tested compounds were utilized during the growth not only as sulfur source but as a source of carbon and nitrogen as well. In four substrates excess sulfur was excreted after oxidation into the medium in the form of sulfate. Small amounts of sulfite were also observed. It usually reacted with remaining disulfides in the medium forming S-sulfo compounds (R-S.SO3H). Cystine and djenkolic acid were oxidized rapidly and completely. In contrast, lanthionine and particularly homocystine were oxidized slowly and only after a longer adaptation. To some extent lanthionine was already oxidized extracellularly in the medium. With methionine and its sulfone, excess sulfur was not removed by oxidation but by demethiolation to methane thiol and further volatile products.

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Über den Schwefelstoffwechsel von Microsporum canis*

Cited by 19 publications

References 14 publications

Antifungal Effects of Selenocystine and its Derivatives on Dermatophytes: Die antimyzetische Wirkung von Selenocystin und seinen Derivaten auf Dermatophyten

Antifungal Effects of Selenocystine and its Derivatives on Dermatophytes: Die antimyzetische Wirkung von Selenocystin und seinen Derivaten auf Dermatophyten

Keratin decomposition by dermatophytes. I. Sulfite production as a possible way of substrate denaturation

Metabolism of sulfur‐containing amino acids in the dermatophyte Microsporum gypseum I. Neutral amino acids

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