Metabolism of sulfur‐containing amino acids in the dermatophyte Microsporum gypseum I. Neutral amino acids

1985

J. Basic Microbiol.

Self Cite

The dermatophyte Microsporum gypseum was cultivated on a glucose-arginine medium supplemented with five strongly acidic derivatives of cysteine (L-cysteine sulfinic acid, L-cysteic acid, L-serine-O-sulfate and taurine at a concentration of 5 mmol/l, and L-S-sulfocysteine at a concentration of 2.5 mmol/l). The addition of these substances did not stimulate the growth as compared with the control containing 0.5 mmol/l cystine. Cysteine sulfinic acid and cysteic acid showed rather inhibitory effects. A strong inhibition of the growth was caused by the presence of serine sulfate. During the growth, all substances investigated were gradually consumed and utilized not only as a source of sulfur but of nitrogen and carbon as well. Cysteine sulfinic acid and S-sulfocysteine were utilized most rapidly. Cysteic acid was also rapidly utilized but after a certain adaptation. Taurine was utilized slowly and serine sulfate very slowly. Excess sulfur contained in the substances used was excreted into the medium in the form of sulfate. Sulfate excretion was most rapid with cysteine sulfinic acid and slowest with taurine. With cysteine sulfinic acid, S-sulfocysteine and cysteic acid, small amounts of sulfite were found in the medium. The results obtained are in accordance with the presumption that cysteine sulfinic acid (but not cysteic acid and taurine) is an intermediate of cysteine catabolism in dermatophytes.

Section: Discussionmentioning

confidence: 97%

“…The consumption of amino acids from the medium and possible formation of other ninhydrin-positive products was detected by chromatography. All the methods used have been described in Part I (KUNERT 1985). L-cysteic acid was a product of REANAL (Budapest, Hungary) and taurine of hBA-Chemie (Vienna, Austria).…”

Section: Methodsmentioning

confidence: 99%

Metabolism of sulfur‐containing amino acids in the dermatophyte Microsporum gypseum II. Acidic amino acid derivatives

1985

J. Basic Microbiol.

Self Cite

Utilization of various concentrations of free cystine by the fungus Microsporum gypseum

1987

J. Basic Microbiol.

The dermatophyte Microsporum gypseum was cultivated on two liquid media enriched with 50 to 1000 micrograms/ml free L-cystine. The presence of cystine in concentrations above 250 micrograms/ml (gelatin medium) or 500 micrograms/ml (glucose-glutamate medium) inhibited the growth. In all variants, however, cystine was utilized from the very beginning of growth and exhausted completely until stationary phase. The rate of cystine metabolization grew with its concentration to 500 micrograms/ml but decreased again with 1000 micrograms/ml. The excess sulfur was oxidized and excreted back into the medium mainly as inorganic sulfate. Moreover, sulfite was also produced which immediately reacted with the residual cystine in the medium giving rise to S-sulfocysteine. Sulfite excretion was higher in the initial phases of growth and on the medium with poorer growth (gelatin medium). The sulfate-to-sulfite ratio was different on the two media used but was little influenced by cystine concentration. The excretion of strongly acidic compounds (sulfate, sulfite, and S-sulfocysteine) reduced the usual alkalinization of the medium in the course of growth.

Cystine catabolism in mycelia of Microsporum gypseum, a dermatophytic fungus

Trüper

1986

Arch. Microbiol.

The fate of 35S label was studied during cystine degradation by mycelia of the dermatophytic fungus Microsporum gypseum. Excess free cystine in the medium was readily taken up and its sulfur moiety excreted as inorganic sulfate and sulfite. At intervals after 3-60 min of incubation with 35S cystine the products of cystine catabolism were extracted from the mycelia by boiling water and separated by thin layer chromatography and electrophoresis. A total of 10 sulfur-containing compounds were identified, and their relative radioactivity was assessed. After 3 min the mycelia contained, in addition to cystine, labeled cysteine and particularly cysteine sulfinic acid which was accompanied by a smaller amount of cysteic acid. Later on, oxidized and reduced glutathione, inorganic sulfate and taurine appeared consecutively. In all extracts, small amounts of labeled S-sulfocysteine were found, not, however, sulfite. The results suggest that the intermediates of cysteine degradation in the fungal mycelia are cysteine, cysteine sulfinate, unstable sulfinylpyruvate, sulfite and sulfate, i.e., that the catabolic pattern is similar to that of higher organisms. The formation and the role of S-sulfocysteine, cysteic acid, and of taurine is not yet completely understood, although certainly autoxidative processes are involved in the formation of the latter two compounds, and sulfitolysis in that of the former compound.