Über den Einbau von C<sup>14</sup>O<sub>2</sub>in Weizenblätter nach Infektion mit Puccinia graminis tritici).

Sydow, B.

doi:10.1111/j.1439-0434.1966.tb02250.x

Cited by 10 publications

(8 citation statements)

References 20 publications

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“…T h e activity of this enzyme is considerably higher in extracts from rustinoculated leaves than in those from healthy leaves (Table V) with labelled substrates reported by von Sydow (1964), and our own results utilizing sucrose-U-14C (see below), present supporting evidence for the increase of invertase activity in vivo after infection with rust. \Ve believe t h a t the values obtained with the complete assay system (assay a in Table V) probably give some information about the invertase concentration in the crude extract.…”

Section: Invertase Activity In Crude Extracts From Ilealthy Leaves Ansupporting

confidence: 61%

“…After application of 14C02 t o wheat leaves, this sugar is one of the earliest radioactive metabolites (von Sydow 1964). Since both glucose and fructose, the moieties of sucrose, are substrates for hexokinase, the rates of sucrose synthesis and utilization may well be determining factors for the hexokinase activity in vivo.…”

Section: Invertase Activity In Crude Extracts From Ilealthy Leaves Anmentioning

confidence: 99%

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Effect of Rust Infection on Hexokinase Activity and Carbohydrate Dissimilation in Primary Leaves of Wheat

Lunderstädt¹

1966

Can. J. Bot.

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The increase in activity of hexolcinase occurring in leaves of wheat (Triticum satat~nt L., variety Little Club) after infection with stem rust (P,zlccinia graminis (Pets.) f. sp. tritizi Erikss. and Henn.) cannot be attributed t o activation, or relief of inhibition, by compounds of low molecular weight in the host-parasite complex. The light regime t o which plants were subjected after inoculation was found t o modify the activity of this enzyme in crude extracts, presumably b y affecting the concentration of inhibitory substances. Fractionation of proteinaceous material by gel filtration produced evidence that infection results in qualitative differences of the hexol~inase protein: the enzyme preparation obtained from infected leaves differs in its behavior on Sephadex G-100 columns from hexokinase preparations isolated from healthy leaves, approximating the gel filtration properties of rust spore hexoltinase. The activity in vitro of invertase, glucohexolcinase, and fructohesolcinase was found t o be correlated with the concentration of their respective substrates in the host-parasite complex: after infection, a small increase of endogenous substrate concentration was accompanied by a small increase of the enzyme activity in vitro; and vice versa. Measurements of invertase activity in vitro indicated, and feeding of sucrose-U-14C t o detached leaves confirmed, that sucrose dissimilation is increased after infection with rust. As measured in crude enzyme preparations, the postinfectional increase of invertase activity was always larger than that of either glucohexokinase or fructohexol

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Section: Invertase Activity In Crude Extracts From Ilealthy Leaves Ansupporting

confidence: 61%

Section: Invertase Activity In Crude Extracts From Ilealthy Leaves Anmentioning

confidence: 99%

Effect of Rust Infection on Hexokinase Activity and Carbohydrate Dissimilation in Primary Leaves of Wheat

Lunderstädt¹

1966

Can. J. Bot.

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“…A number of papers has provided evidence for accumulation of radioactive substances at infection sites (Shaw et al 1954;Shaw and Samborski 1956;Thrower 1965;Livne and Daly 1966;Pozsar and Kiraly 1966;von Sydow 1966;Thrower 1965 ;and Garraway and Pelletier 1966) in fungal hyphae in or over disease lesions (Bhattacharya and Shaw 1967;Jager and Reisener 1969a, b;and Mount and Ellingboe 1969).…”

mentioning

confidence: 96%

Microradioautography of potato leaves inoculated with ³H-labeled conidia of Phytophthora infestans

Sivak¹,

Shaw²

1970

Can. J. Bot.

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SIVAK, B., and M. SHAW. 1970. Microradioautography of potato leaves inoculated with 3H-labeled conidia of Plzytophthorn infestans. Can. J . Bot. 48: 613-615. Leaves of Solartlrm tuberosum var. Green Mountain were inoculated with leucine-3H or cytidine-3H labeled spores of Phytophthorn infestans. Microradioautography showed that such spores germinated and developed an extensive radioactive intercellular mycelium but the release of radioactive substances, and their transfer to host organelles were not detected. Possible explanations are discussed briefly.A number of papers has provided evidence for accumulation of radioactive substances at infection sites (Shaw et al.

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“…The large size of individual pustules produced by P. poarum facilitates the investigation of the transfer process and, following photosynthesis, marked accumulation occurs in individual sites of infection in both detached leaves and leaf discs as noted earlier by others for several biotrophic pathogens (see e.g. Shaw and Samborski, 1956;Thrower, 1965b;von Sydow, 1966a;Gaunt and Manners, 1971;Thrower & Lewis, 1973). A significant qualitative difference between infection of Tussilago by Puccinia poarum and those previously studied is the accumulation, in tissue of Tussilago at infection sites, of polyfructan (inulin) instead of the stimulated synthesis of polyglucose (starch) as in tissues of hosts to other biotrophs .…”

Section: Discussionmentioning

confidence: 99%

Carbohydrate Metabolism in Healthy and Rusted Leaves of Coltsfoot

1974

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Summary Infection of leaves of Tussilago by Puccinia poarum results in decreased export of photosynthetic products and, when infection is severe, in increased import. Photosynthetic products move to infection sites where host metabolites are converted to specific fungal products, including mannitol, arabitol, trehalose, glycogen, glucomannan and lipids. More previously fixed 14C is translocated to the fungus in the dark than in the light since, in the dark, degradation of starch contributes to net movement of 14C.

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Über den Einbau von C¹⁴O₂in Weizenblätter nach Infektion mit Puccinia graminis tritici).

Cited by 10 publications

References 20 publications

Effect of Rust Infection on Hexokinase Activity and Carbohydrate Dissimilation in Primary Leaves of Wheat

Effect of Rust Infection on Hexokinase Activity and Carbohydrate Dissimilation in Primary Leaves of Wheat

Microradioautography of potato leaves inoculated with ³H-labeled conidia of Phytophthora infestans

Carbohydrate Metabolism in Healthy and Rusted Leaves of Coltsfoot

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Über den Einbau von C14O2in Weizenblätter nach Infektion mit Puccinia graminis tritici).

Cited by 10 publications

References 20 publications

Effect of Rust Infection on Hexokinase Activity and Carbohydrate Dissimilation in Primary Leaves of Wheat

Effect of Rust Infection on Hexokinase Activity and Carbohydrate Dissimilation in Primary Leaves of Wheat

Microradioautography of potato leaves inoculated with 3H-labeled conidia of Phytophthora infestans

Carbohydrate Metabolism in Healthy and Rusted Leaves of Coltsfoot

Contact Info

Product

Resources

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Über den Einbau von C¹⁴O₂in Weizenblätter nach Infektion mit Puccinia graminis tritici).

Microradioautography of potato leaves inoculated with ³H-labeled conidia of Phytophthora infestans