Microradioautography of potato leaves inoculated with ³H-labeled conidia of Phytophthora infestans

Abstract

SIVAK, B., and M. SHAW. 1970. Microradioautography of potato leaves inoculated with 3H-labeled conidia of Plzytophthorn infestans. Can. J . Bot. 48: 613-615. Leaves of Solartlrm tuberosum var. Green Mountain were inoculated with leucine-3H or cytidine-3H labeled spores of Phytophthorn infestans. Microradioautography showed that such spores germinated and developed an extensive radioactive intercellular mycelium but the release of radioactive substances, and their transfer to host organelles were not detected. Possible explanations are discussed briefly.A number of papers has provided evidence for accumulation of radioactive substances at infection sites (Shaw et al.

Microautoradiographic studies on host-parasite interactions II. The exchange of 3H-lysine between Uromyces phaseoli and Phaseolus vulgaris

“…This corresponds to earlier work with 3H-orotic acid (Mendgen and Heitfuss, 1975) and to results observed after infection of Solanum tuberosum with Phytophthora infestans (Sivak and Shaw, 1970). Such studies, however, cannot exclude the possibility that secretion or transfer of minor amounts of label from the parasite occurs, as it is difficult in electronmicroscopic autoradiography to differentiate a few silver grains over a large structure from background grains (Mendgen and Heitefuss, 1975).…”

“…kinetics of metabolite uptake during development and aging of haustoria and on the possible transfer of similar metabolites from fungus to host tissue at the early stage of infection (see Sivak and Shaw, 1970;Mendgen and Heitefuss, 1975). Such information is needed for a description of haustorial function.…”