Über den Chemismus der Farbreaktion von 2‐Thiobarbitursäure mit Carbonyl‐Verbindungen

Abstract

Anhand der Ergebnisse systematischer Untersuchungen uber die Reaktion zwiscben 2-Thiobarbitursaure (TBS) und definierten, als Modelle ausgewahlten Carbonyl-Verbindungen sowie naturlichen Substraten wird iiber die Spezifitat dieser Farbreaktion, iiber Struktur, Eigenschaften und Bildungsbedingungen der entstehenden Farbstoffe sowie uber die analytischen Anwendungsmoglichkeiten berichtet. Chemistry of the Colour-Reaction of 2-Thiobarbituric Acid with Carbonyl CompoundsFrom the results at hand of the systematic investigations over the reaction between 2-thiobarbituric acid (TBS) and definite chosen model-carbonyl-compounds as well as natural substances, the specificity of those colour reactions, structure, properties and combining conditions of the resulting coloured material and the possibilities of analytical use have been reported.

Analysis of fat deterioration‐comparison of some photometric tests

“…Numerous variations in the procedure of the TBA test are suggested in the literature (2). To increase the sensitivity, an addition of Fe +3 ions (19,20) is recommended. The reason for higher values is due to the breakdown of peroxides to products which react with TBA (9,21).…”

“…The reason for higher values is due to the breakdown of peroxides to products which react with TBA (9,21). Besides this, the possibility cannot be excluded that under drastic reaction conditions the sample is further autoxidized or that fat accompanying substances influence the TBA test (19,22). Since in this case there is no real increase in sensitivity, a procedure for the TBA reaction (9,10) was chosen in which acid and Fe + 3 ions were not present.…”

Copper‐dependent hydroxyl radical damage to ascorbic acid

“…In these test systems it appears that a TBA adduct is formed with malondialdehyde (MDA) which is released from precursor molecules during the a&d heating stage of the test [2,3]. Several other aldehydes as well as the bile pigments give similar chromogenic adducts with TBA [4,5]. However, these can be distinguished from the MDA-TBA adduct following chromatographic separation or characterisation of their fluorescent spectra [61.…”

“…The OH' radical is probably formed by the reaction sequence shown in eq. (3)(4)(5). Autoxidation of Cu(I) will lead to the formation of superoxide radicals which rapidly dismutate to form hydrogen peroxide.…”

Thiobarbituric acid test for detecting lipid peroxides

“…However, secondary products from linoleic acid also form a red pigment with the TBA test. It has been reported that 2,4-alkadienal forms the red pigment, but the red pigment formed by 2,4-alkadienal was suggested to be caused by further oxidation of alkadienal to malonaldehyde (16)(17)(18)(19)(20)(21).…”