Über das gemeinsame Vorkommen von Triterpenen und Sterinen im Blatt von Salvia triloba und Pirus malus 6. Mitteilung zur Kenntnis der Biochemie der Triterpene

Brieskorn, Carl-Hein Z.; Klinger, Helmut; Polonius, Wulfran

doi:10.1002/ardp.19612940702

Cited by 13 publications

(3 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, selective and innovative isolation techniques are necessary to fully exploit the selective recovery of triterpenes which have gained increasing interest. Especially the pentacyclic triterpenes oleanolic acid (OA) and ursolic acid (UA) are attractive due to their demonstrated biological effects like anti-inflammation, hepatoprotection, antihyperglucemia, and antitumor promotion [4,5]. Both substances have an identical molecular weight and differ only in the position of one methyl group in their chemical structure (see Fig.…”

Section: Introductionmentioning

confidence: 99%

Plant Extraction with Aqueous Two‐Phase Systems

et al. 2011

View full text Add to dashboard Cite

The extraction of phytochemicals from plants and their downstream processing is often associated with solvents, which are problematical with respect to health, safety, and environment. An alternative approach is the extraction with aqueous two-phase systems, which offers a benign environment for complex molecular structures. Particularly nonionic surfactants like, e.g., Genapol® X-080 and Tween® 65 in high concentrations in water can be used to form aqueous twophase systems. Sage (Salvia triloba) is used as a model plant, since it contains a relatively high concentration of the two triterpenes oleanolic acid (OA) and ursolic acid (UA), which are interesting due to their wide range of biological effects. Kinetic experiments have shown that an extractive isolation of triterpene acids using such mesophase systems is possible.

show abstract

Section: Introductionmentioning

confidence: 99%

Plant Extraction with Aqueous Two‐Phase Systems

et al. 2011

View full text Add to dashboard Cite

show abstract

“…This can be explained by the occurrence of the triterpenes OA and UA in Salvia triloba, which are located on the leaf surface between the cuticle and the epidermal cell. [24] Due to the fact that surfactants are surface active agents and therefore the penetration into the plant cells is lower or a more time-intensive process compared to organic solvents, the accessibility for these triterpenes is higher compared to the undesired by-compounds and results in less contaminants. The lower hazard potential of the aqueous two-phase system compared to organic solvents used in industrial extractions for these triterpenes is a further advantage.…”

Section: Extraction With Surfactant/water Mixturesmentioning

confidence: 99%

Surfactant Mediated Extraction of Triterpenes and Their Direct HPLC Analysis From the Micellar System

Schneider

Hosseiny

Bischoff

et al. 2011

Instrumentation Science & Technology

View full text Add to dashboard Cite

& The pentacyclic triterpenes oleanolic acid (OA) and ursolic acid (UA) in Salvia triloba are attractive ingredients for cosmetic and pharmaceutical formulations. These triterpenoids are constitution isomers and differ only in the position of one methyl group in their chemical structures. Therefore it is difficult to achieve a baseline separation by standard RP-HPLC systems. Another challenge is that the complex plant contains a large variety of similar compounds. To achieve precise results for the target molecules, these compounds should be removed prior to analysis. Due to the large application of these two triterpenes, a fast quantification method is favored. If the triterpenes are extracted with aqueous two-phase systems, the target substances are enriched in a surfactant-rich phase. For this application, it is desirable to develop a method that is capable of quantifying both triterpenes directly by RP-HPLC without additional purification steps.The optimal chromatographic conditions for pure substances as well as for crude surfactantcontaining extracts were accomplished on a Nucleodur C18 ISIS column by isocratic elution with methanol=water=acetic acid=triethanolamine (90:10:0.04:0.02 v=v) as the mobile phase and a column temperature of 10 C. The flow rate was 0.6 mL=min, and the detection wavelength was 210 nm. With these parameters, a baseline separation was achieved and the calibration curve showed a very good linearity (R 2 > 0.999 for OA and UA) within the test range. The method is simple, rapid, and reliable for the quantification of crude extracts from plant material.

show abstract

“…/3-Sitosterol, campesterol and stigmasterol occur in apple buds. /3-Sitosterol and campesterol have been found in apple leaves (Brieskorn et al 1961), seed, peel and pulp (Bock et al 1966, Sawai et al 1978. The sterol indicates that sterol synthesis is rapid in actively growing and differentiating plant tissue.…”

Section: Sterol Contentmentioning

confidence: 99%

Changes of fatty acids and sterols in apple buds during bud break induced by a plant bioregulator, thidiazuron

Wang

Faust

1988

Physiologia Plantarum

View full text Add to dashboard Cite

The effects of N‐phenyl‐N'‐l,2,3,‐thidiazol‐5‐ylurea (thidiazuron; Dropp; SN 49537) on fatty acids of membrane lipids and sterol content in apple (Malus domestica Borkh cv. Golden Delicious) buds associated with bud break and bud development were determined. The predominant fatty acids in the membrane lipids of apple buds were palmitic acid (C16:0), linoleic acid (C18:2) and linolenic acid (C18:3). β‐Sitosterol and sitosteryl ester were the predominant sterols. An accumulation of unsaturated polar membrane fatty acids started after thidiazuron treatment. A decrease in the percentage of the sitosterol was accompanied by an increase in campesterol and stigmasterol at the beginning of rapid growth. An increase in the ratio of campesterol and stigmasterol to sitosterol and a decrease in the ratio of free sterols to membrane lipids upon breaking of dormancy also occurred in apple buds induced by thidiazuron.

show abstract

Über das gemeinsame Vorkommen von Triterpenen und Sterinen im Blatt von Salvia triloba und Pirus malus 6. Mitteilung zur Kenntnis der Biochemie der Triterpene

Cited by 13 publications

References 5 publications

Plant Extraction with Aqueous Two‐Phase Systems

Plant Extraction with Aqueous Two‐Phase Systems

Surfactant Mediated Extraction of Triterpenes and Their Direct HPLC Analysis From the Micellar System

Changes of fatty acids and sterols in apple buds during bud break induced by a plant bioregulator, thidiazuron

Contact Info

Product

Resources

About