Changes of fatty acids and sterols in apple buds during bud break induced by a plant bioregulator, thidiazuron

Wang, S. Y.; Faust, Miklos

doi:10.1111/j.1399-3054.1988.tb06631.x

Cited by 31 publications

(14 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, studying apple bud break, Wang and his co-workers (Wang et al 1991a(Wang et al , 1991b found changes in key enzymes and other regulatory elements in response to TDZ. There are further indications that TDZ also induces accumulation of unsaturated polar membrane fatty acids (Wang and Faust 1988). This line of research strongly suggests that TDZ is actively involved in inducing metabolic changes and that it is not a simple substitute of purine-based cytokinins.…”

Section: Discussionmentioning

confidence: 99%

Thidiazuron Induces High-Frequency Shoot Regeneration in Intact Seedlings of Pea (Pisum sativum), Chickpea (Cicer arietinum) and Lentil (Lens culinaris)

Malik¹,

Saxena²

1992

Functional Plant Biol.

129

View full text Add to dashboard Cite

Axenic seedling cultures of chickpea (Cicer arietinum L.), lentil (Lens culinaris Medik.) and garden pea (Pisum sativum L.) were established by culturing mature seeds on Murashige and Skoog medium (MS) supplemented with thidiazuron (TDZ). Of various cytokinins or compounds with cytokinin-like activity (Kinetin, TDZ, Zeatin) tested for inducing shoot formation in pea seeds cultures, TDZ was found t o be most effective. Pea seedlings exhibited a unique pattern of shoot formation which was accomplished in two distinct phases. Multiple shoots developed within a week, from the nodal and basal regions of the primary epicotyl in a medium that contained 5-50 I(M TDZ. When these seedlings were exposed for a prolonged time period (3-4 weeks), to the same medium, numerous shoot buds emerged de novo from the base and/or from the upper part of multiple shoots. These shoots had no apparent vascular connection with parent tissues. The inductive capability of TDZ was then tested in several other genotypes of Pisum sativum and two other large-seeded grain legumes, Cicer arietinum, and Lens culinaris. In Cicer arietinum, and Lens culinaris, multiple shoots developed after 1 week of seed culture on media that contained 1-50 I(M TDZ. However, de novo differentiation of shoot buds occurred in cultures exposed to TDZ for 4-6 weeks, only from nodal and subjacent areas. Secondary shoot formation occurred frequently in all of the species tested. Developing shoots were able to form roots and eventually whole plants on a modified MS medium containing 2.5 I(M NAA. No genotypic difference for morphogenesis was observed.

show abstract

Section: Discussionmentioning

confidence: 99%

Thidiazuron Induces High-Frequency Shoot Regeneration in Intact Seedlings of Pea (Pisum sativum), Chickpea (Cicer arietinum) and Lentil (Lens culinaris)

Malik¹,

Saxena²

1992

Functional Plant Biol.

129

View full text Add to dashboard Cite

show abstract

“…Wang et al (1991) found changes in key enzymes and other regulatory elements in response to TDZ. Wang and Faust (1988) observed that TDZ induces accumulation of unsaturated polar membrane fatty acids, which strongly propose the active involvement of TDZ in inducing metabolic changes and suggested that it is not a simple substitute for purine based cytokinins. Also Sharma et al (2008) analyzed effect of BAP, indole-butyric acid (IBA) and sucrose on shoot regeneration in C. sativus.…”

mentioning

confidence: 96%

Activity of antioxidant enzyme during in vitro organogenesis in Crocus sativus

Vatankhah¹,

Niknam²,

Ebrahimzadeh³

2010

Biol. plant.

View full text Add to dashboard Cite

The effect of various hormonal combinations on regeneration of shoots and roots from meristem-derived callus of Crocus sativus L. and activities of antioxidant enzymes have been studied. The most efficient regeneration occurred with 1.0 mg dm -3 1-naphthaleneacetic acid (NAA) + 1.0 mg dm -3 thidiazuron and 1.0 mg dm -3 NAA + 2.0 mg dm -3 kinetin. For sprouting, regenerated shoot were subcultured on Murashige and Skoog medium containing 1.0 mg dm -3 NAA + 1.0 mg dm -3 benzylaminopurine (BAP). Protein content and superoxide dismutase activity decreased in regenerated shoots and roots and increased in sprouting shoots, while catalase (CAT), peroxidase (POX) and polyphenol oxidase (PPO) activities increased during organogenesis and decreased in sprouting shoots. High CAT and PPO activities were detected in regenerated roots, whereas high POX activity was observed in regenerated shoot.Additional key words: catalase, peroxidase, polyphenol oxidase, protein, saffron, superoxide dismutase.

show abstract

“…This cold requirement can be overcome by treating the buds with certain plant hormones and growth regulators. We previously reported that the plant bioregulant thidiazuron (N-phenyl-N'-l,2,3-thidiazol-5-ylurea) has the capacity to release apple lateral buds from dormancy and to induce metabolic changes (Wang and Faust 1988a, Wang et al 1986. A number of studies demonstrated that the physiological alterations produced in plants due to different causes are related to enzymes that catalyze oxidative reactions.…”

mentioning

confidence: 99%

Changes in the activities of catalase, peroxidase, and polyphenol oxidase in apple buds during bud break induced by thidiazuron

Wang

Jiao

Faust

1991

J Plant Growth Regul

View full text Add to dashboard Cite

Abstract. The breaking of dormancy in apple buds (Malus domestica Borkh cv. York Imperial) by thidiazuron (N-phenyl-N'-1,2,3,-thidiazol-5-ylurea) was investigated in relation to catalase, peroxidase, and polyphenol oxidase activities and their isoenzyme patterns. The activity and number of isoenzymic components of catalase increased progressively during bud break, then decreased after buds started to grow. Peroxidase activity was highest during dormancy and declined during bud swell, increased at bud break, and decreased after bud expansion. Several isoperoxidases were observed in gel electrophoresis. Similar patterns were found at different growth stages of apple buds except for one peroxidase isoenzyme, P3, which disappeared 12 days after thidiazuron treatment. There was an inverse relationship between the activities of polyphenol oxidase and peroxidase during the development of apple buds. Apple buds have a very similar polyphenol oxidase isoenzyme pattern throughout bud development. However, the appearance and disappearance of minor isoenzymes were also observed. Phloridzin, rutin, p-coumaric, epicatechin, naringin, chlorogenic acid, and catechol were found in apple buds. Among them, phloridzin, rutin, and p-coumaric were the dominant phenolic compounds. Dormant buds contained a high amount of phenolic substances which decreased after bud break (4 days after thidiazuron treatment) then increased until the start of bud expansion. Phenolic compounds are found to be potent modifiers of catalase, peroxidase, and polyphenol oxidase activity, as both inhibitors and stimulators in apple buds.

show abstract

Changes of fatty acids and sterols in apple buds during bud break induced by a plant bioregulator, thidiazuron

Cited by 31 publications

References 29 publications

Thidiazuron Induces High-Frequency Shoot Regeneration in Intact Seedlings of Pea (Pisum sativum), Chickpea (Cicer arietinum) and Lentil (Lens culinaris)

Thidiazuron Induces High-Frequency Shoot Regeneration in Intact Seedlings of Pea (Pisum sativum), Chickpea (Cicer arietinum) and Lentil (Lens culinaris)

Activity of antioxidant enzyme during in vitro organogenesis in Crocus sativus

Changes in the activities of catalase, peroxidase, and polyphenol oxidase in apple buds during bud break induced by thidiazuron

Contact Info

Product

Resources

About