In this paper, U(3) L ×U(3) R chiral theory of mesons is extended to the leading order in quark mass expansion in order to evaluate the ρ 0 − ω mixing. It is shown that the use of path integral method to integrate out the quark fields naturally leads to the ρ 0 − ω mixing vertices, and this mixing is entirely from the quark loop in this theory. The off-shell behaviour of the mixing amplitude is analyzed. The on-shell mixing amplitude is obtained from the decay ω → π + π − . Furthermore, the constraints on the light quark mass parameters are extracted from the ρ 0 − ω mixing and the mesons masses, and the mass splitting of K * (892)-mesons is predicted.