Tyrosol prevents apoptosis in irradiated keratinocytes

Salucci, Sara; Burattini, Sabrina; Battistelli, Michela; Buontempo, Francesca; Canonico, Barbara; Martelli, Alberto M.; Papa, Stefano; Falcieri, Elisabetta

doi:10.1016/j.jdermsci.2015.07.002

Cited by 44 publications

(27 citation statements)

References 29 publications

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“…We suggest that sperm quality improvement after washing could be explained by ROS elimination as HT acts as a scavenger by breaking the chain of reactions triggered by free radicals (Visioli et al ., ; Tripoli et al ., ; Hamden et al ., ). Antioxidant activities of HT were tested in some experimental cell‐based models such as in human erythrocytes (Paiva‐Martins et al ., ), keratinocytes (Salucci et al ., ) and other cell types (Raederstorff, ). It was also shown that HT at concentrations varying from 0.05 m m to 1 m m inhibits the formation of DNA oxidation products in neuronal hybridoma cell line (Deiana et al., ).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant effect of hydroxytyrosol on human sperm quality duringin vitroincubation

Kedechi

Zribi

Louati³

et al. 2016

Andrologia

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The aim of the study was to evaluate the antioxidant potential of hydroxytyrosol (HT) on human sperm quality during incubation in vitro. Semen samples collected from men attending the Laboratory of Histology-Embryology of Sfax Faculty of Medicine (Tunisia) for infertility investigations were evaluated for initial sperm parameters. Only normal selected ejaculates (n = 15) were centrifuged and incubated further with or without HT (200ug ml ) at room temperature for 45 min. After incubation, sperm motility and viability, DNA oxidation and reactive oxygen species (ROS) production were assessed. The results showed that centrifugation significantly influenced sperm motility and viability. The supplementation of HT in incubating media improved (P = 0.01) significantly sperm viability and decreased sperm DNA oxidation (P < 0.001) and ROS levels (P = 0.03) following centrifugation. It can be concluded that supplementation of HT might be helpful to maintain the human spermatozoon after centrifugation.

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Section: Discussionmentioning

confidence: 99%

Antioxidant effect of hydroxytyrosol on human sperm quality duringin vitroincubation

Kedechi

Zribi

Louati³

et al. 2016

Andrologia

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“…Antioxidant concentrations have been chosen since they did not affect HaCaT cell viability and proliferation as demonstrated in our previous works. 14 , 24 …”

Section: Methodsmentioning

confidence: 99%

Protective effect of different antioxidant agents in UVB-irradiated keratinocytes

et al. 2017

Self Cite

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Skin cells can respond to UVB-induced damage either by tolerating it, or restoring it through antioxidant activation and DNA repair mechanisms or, ultimately, undergoing programmed cell death, when damage is massive. Nutritional factors, in particular, food antioxidants, have attracted much interest because of their potential use in new preventive, protective, and therapeutic strategies for chronic degenerative diseases, including skin inflammation and cancer. Some polyphenols, present in virgin olive oil, well tolerated by organism after oral administration, show a variety of pharmacological and clinical benefits such as antioxidant, anti-cancer, anti-inflammatory, and neuro-protective activities. Here, the protective effects of antioxidant compounds against UV-induced apoptosis have been described in HaCaT cell line. Human keratinocytes were pre-treated with antioxidants before UVB exposure and their effects have been evaluated by means of ultrastructural analyses. After UVB radiation, a known cell death trigger, typical apoptotic features, absent in control condition and in antioxidant alone-treated cells, appear. An evident numerical decrease of ultrastructural apoptotic patterns and TUNEL positive nuclei can be observed when natural antioxidants were supplied before cell death induction. These data have been confirmed by molecular investigation of caspase activity. In conclusion, this paper highlights antioxidant compound ability to prevent apoptotic cell death in human keratinocytes exposed to UVB, suggesting, for these molecules, a potential role in preventing skin damage.

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“…Cell growth experiments were performed using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay with minor modifications to the previously described procedure [7, 8, 24, 25]. Hydrogen peroxide was used as the positive control.…”

Section: Methodsmentioning

confidence: 99%

N-(4-methoxyphenyl) caffeamide-induced melanogenesis inhibition mechanisms

Kuo

Chen

et al. 2017

BMC Complement Altern Med

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BackgroundThe derivative of caffeamide exhibits antioxidant and antityrosinase activity. The activity and mechanism of N-(4-methoxyphenyl) caffeamide (K36E) on melanogenesis was investigated.MethodsB16F0 cells were treated with various concentrations of K36E; the melanin contents and related signal transduction were studied. Western blotting assay was applied to determine the protein expression, and spectrophotometry was performed to identify the tyrosinase activity and melanin content.ResultsOur results indicated that K36E reduced α-melanocyte-stimulating hormone (α-MSH)-induced melanin content and tyrosinase activity in B16F0 cells. In addition, K36E inhibited the expression of phospho-cyclic adenosine monophosphate (cAMP)-response element-binding protein, microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related protein-1 (TRP-1). K36E activated the phosphorylation of protein kinase B (AKT) and glycogen synthase kinase 3 beta (GSK3β), leading to the inhibition of MITF transcription activity. K36E attenuated α-MSH induced cAMP pathways, contributing to hypopigmentation.ConclusionsK36E regulated melanin synthesis through reducing the expression of downstream proteins including p-CREB, p-AKT, p-GSK3β, tyrosinase, and TRP-1, and activated the transcription factor, MITF. K36E may have the potential to be developed as a skin whitening agent.

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Tyrosol prevents apoptosis in irradiated keratinocytes

Cited by 44 publications

References 29 publications

Antioxidant effect of hydroxytyrosol on human sperm quality duringin vitroincubation

Antioxidant effect of hydroxytyrosol on human sperm quality duringin vitroincubation

Protective effect of different antioxidant agents in UVB-irradiated keratinocytes

N-(4-methoxyphenyl) caffeamide-induced melanogenesis inhibition mechanisms

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