Tyrosinase mRNA levels in the blood of uveal melanoma patients: correlation with the number of circulating tumor cells and tumor progression

Pinzani, Pamela; Mazzini, Cinzia; Salvianti, Francesca; Massi, Daniela; Grifoni, Raffaella; Paoletti, Costanza; Ucci, Francesca; Molinara, Elena; Orlando, Claudio; Pazzagli, Mario; Neri, B

doi:10.1097/cmr.0b013e32833906e3

Cited by 39 publications

(44 citation statements)

References 27 publications

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“…Serum tyrosinase m-RNA levels have been shown to be increased in patients with primary uveal melanoma, and they correlate with metastatic disease. In addition, tyrosinase m-RNA can be used for the indirect quantification of circulating tumour cells and have been correlared with the dimensions of the primary tumour [61]. …”

Section: Metastasesmentioning

confidence: 99%

Uveal Melanoma

Papastefanou

Cohen

2011

Journal of Skin Cancer

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Uveal melanoma is the most common primary intraocular malignancy and the leading primary intraocular disease which can be fatal in adults. In this paper epidemiologic, pathogenetic, and clinical aspects of uveal melanoma are discussed. Despite the advance in local ocular treatments, there has been no change in patient survival for three decades. Development of metastases affects prognosis significantly. Current survival rates, factors predictive of metastatic potential and metastatic screening algorithms are discussed. Proposed and emerging treatments for uveal melanoma metastases are also overviewed. Current advances in genetics and cytogenetics have provided a significant insight in tumours with high metastatic potential and the molecular mechanisms that underlie their development. Biopsy of those lesions may prove to be important for prognostication and to allow further research into genetic mutations and potential new therapeutic targets in the future.

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Section: Metastasesmentioning

confidence: 99%

Uveal Melanoma

Papastefanou

Cohen

2011

Journal of Skin Cancer

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“…In a previous paper [8] we studied a group of 41 patients affected by uveal melanoma including small, medium and large size tumors (Collaborative Ocular Melanoma [9]) followed for a period of up to 55 months. The study was performed by RT-qPCR for the measurement of tyrosinase mRNA in blood as a means to detect and indirectly quantify the number of CTC.…”

Section: Introductionmentioning

confidence: 99%

Circulating Tumor Cells Detection and Counting in Uveal Melanomas by a Filtration-Based Method

Mazzini

Pinzani

Salvianti

et al. 2014

Cancers

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Uveal melanoma is one of the most deadly diseases in ophthalmology for which markers able to predict the appearance of metastasis are needed. The study investigates the role of circulating tumor cells (CTC) as a prognostic factor in this disease. We report the detection of circulating tumor cells by Isolation by Size of Epithelial Tumor cells (ISET) in a cohort of 31 uveal melanoma patients: we identified single CTCs or clusters of cells in 17 patients, while the control population, subjects with choroidal nevi, showed no CTC in peripheral blood. The presence of CTCs did not correlate with any clinical and pathological parameter, such as tumor larger basal diameter (LBD), tumor height and TNM. By stratifying patients in groups on the basis of the number of CTC (lower or higher than 10 CTC per 10 mL blood) and the presence of CTC clusters we found a significant difference in LBD (p = 0.019), Tumor height (p = 0.048), disease-free and overall survival (p < 0.05). In conclusion, we confirm the role of CTC as a negative prognostic marker in uveal melanoma patients after a long follow-up period. Further characterization of CTC will help understanding uveal melanoma metastasization and improve patient management.

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“…Bluemke et al (15) and McKiernan et al (35) also reported that there was no correlation between the presence of CTCs and tumor size in RCC. However, Pinzani et al (36) demonstrated that the messenger RNA levels of tyrosinase, which can be taken as an indirect parameter correlated to the number of CTCs, were significantly correlated with tumor dimension and disease-free and overall survival through reverse transcription-quantitative polymerase chain reaction analysis of blood from 41 patients with uveal melanoma. In the present study, there was no correlation between the positive staining rate and age, gender, tumor size, the pathological pattern, T classification, Fuhrman grade, presence of a lesion or metastasis to the lungs in 27 metastatic patients.…”

Section: Discussionmentioning

confidence: 99%

New applications of the acridine orange fluorescence staining method: Screening for circulating tumor cells

et al. 2017

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The aim of the present study was to explore use of the acridine orange fluorescence (AO-F) staining method for screening of circulating tumor cells (CTCs) in renal cell carcinoma (RCC) patients. The AO-F positive staining rate of live and dead tumor cells was calculated. The positive staining rate in the live group was 93.4±3.0%, while the dead group failed to emit specific fluorescence. A known number of tumor cells were added to peripheral blood, and the detection sensitivity of the four groups (50, 100, 200 and 500 cells/tube) was 10.2±3.8, 9.2±2.3, 10.8±2.6 and 10.5±1.9%, respectively. The average detection sensitivity of the four groups was 10.16±2.73%. There was a positive correlation between the number of cells that was positively stained with AO-F and the total number cells in the system (χ2=0.959; P<0.001). Subsequently, the AO-F staining method was used to detect positive staining cells in 8 healthy volunteers (control group), and 112 non-metastatic and 27 metastatic RCC patients. The positive staining rate was 13.67% (19/139) in RCC patients, while none of the control group was positive. The AO-F positive staining rate was not significantly different between the metastatic and non-metastatic patients according to age, gender, the pathological pattern, T2/3 (according to the Tumor-Node-Metastasis classification) or Fuhrman grade, while there was a significant difference according to T1. The positive staining rate was 8.93% (10/112) for non-metastatic patients and 33.33% (9/27) for metastatic patients, which showed a significant difference (P<0.05). In 112 non-metastatic and 27 metastatic patients, the positive staining rate was not significantly associated with gender, age, tumor size, the pathological pattern, T classification, Fuhrman grade, the presence of a lesion or metastasis to the lungs. The present study demonstrated that the method of CTC staining with AO-F, which has high reproducibility and specificity, was feasible for identifying CTCs and warrants further study.

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Tyrosinase mRNA levels in the blood of uveal melanoma patients: correlation with the number of circulating tumor cells and tumor progression

Cited by 39 publications

References 27 publications

Uveal Melanoma

Uveal Melanoma

Circulating Tumor Cells Detection and Counting in Uveal Melanomas by a Filtration-Based Method

New applications of the acridine orange fluorescence staining method: Screening for circulating tumor cells

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