Typing of Staphylococcus aureus Isolated from Nasal Carriers

Toshkova, K.; Savov, Encho; Indarjulianto, Soedarmanto; Lämmler, Christoph; Chankova, D; Belkum, Alex van; Verbrugh, Henri A.; Leeuwen, Willem van

doi:10.1016/s0934-8840(97)80059-x

Cited by 15 publications

(13 citation statements)

References 19 publications

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“…As shown for patient L this nasal carriage appeared to be stable at least for a 2-year period. These ¢ndings correspond to data presented previously [27]. The identity of the S. aureus isolates in each pair could be demonstrated phenotypically by hemolysin production, their antibiotic resistances, the secreted enterotoxins and by phage-typing.…”

Section: Discussionsupporting

confidence: 89%

The significance of nasal carriage of Staphylococcus aureus as risk factor for human skin infections

Toshkova

2001

FEMS Microbiology Letters

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The present study was designed to investigate the significance and the relationship between nasal carriage of Staphylococcus aureus and staphylococcal skin infections. Thirty-one S. aureus strains, isolated from 12 patients with chronic and recurrent skin infections, one patient with septicemia and one patient with otitis externa were studied. The staphylococcal strains were isolated from the site of infection and from the anterior nares of each patient. The identity of both strains of each pair could be demonstrated by determination of phenotypic properties and by genotyping of the isolates. The phenotypic properties included hemolytic activities, antibiotic resistance data, and the production of enterotoxins. The identity was additionally confirmed by phage-typing, by determination of the size and the number of repeats of the X region of spa gene, by determination of gene polymorphisms of coa gene and by macrorestriction analysis of the chromosomal DNA of the isolates by pulsed-field gel electrophoresis. The present results showed an identity of the S. aureus obtained from anterior nares and from skin infection of each patient indicating the importance of nasal carriage of these bacteria for development of human skin infection. ß

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Section: Discussionsupporting

confidence: 89%

The significance of nasal carriage of Staphylococcus aureus as risk factor for human skin infections

Toshkova

2001

FEMS Microbiology Letters

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“…Additionally, PFGE analysis also demonstrated the same patterns within different carriers (PT C and D) at different times, suggesting that there occurred transference of microorganisms among food workers through direct or indirect contact and consequently this is a potential source of diet contamination. This fact was previously demonstrated by other researchers in healthy person, including personnel staff (Toshkova and others 1997). According to Kennedy and others (2000), the strains that are present in the nose can be transmitted to the hands and skin and may contaminate the air, water, soil, food, and any area or object that has come into contact with the carrier increasing risks of food poisoning.…”

Section: Resultssupporting

confidence: 78%

Molecular Epidemiology of Microorganisms Isolated from Food Workers and Enteral Feeding of Public Hospitals

Borges¹,

Campos²,

Cardoso³

et al. 2010

Journal of Food Science

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“…Ten differentsized amplicons of approximately 90,110,140,170,190,220,240,270,290, and 320 bp and calculated numbers of 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11 repeats, respectively, were observed. Comparable differences in the number of repeats of the X region of the protein A (spa) gene had already been used for genotyping isolates of this species (14,15,46,48).…”

Section: Discussionmentioning

confidence: 99%

“…The isolates were also investigated by digestion of their chromosomal DNAs with the restriction enzyme SmaI and subsequent separation of the fragments by pulsed-field gel electrophoresis (PFGE) using the Chef-Dr II pulsed-field electrophoresis system (Bio-Rad, Munich, Germany). The preparation of the DNA and the program for PFGE were described previously (46). In accordance with the method of Tenover et al (45), restriction patterns with no fragment difference were recorded as indistinguishable, restriction patterns with two to three fragments difference were recorded as closely related, and those with four and more differences were recorded as not related.…”

Section: Methodsmentioning

confidence: 99%

Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis

Akineden

Annemüller

Hassan

et al. 2001

Clin Diagn Lab Immunol

188

143

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In the present study, 103 Staphylococcus aureus strains isolated from milk samples from 60 cows with mastitis from eight different farms in seven different locations in one region of Germany were compared pheno-and genotypically and by identification of various toxins. On the basis of culture and hemolytic properties and by determination of the tube coagulase reaction, all of the isolates could be identified as S. aureus. This could be confirmed by PCR amplification of species-specific parts of the gene encoding the 23S rRNA. In addition, all of the S. aureus isolates harbored the genes encoding staphylococcal coagulase and clumping factor and the genes encoding the X region and the immunoglobulin G binding region of protein A. These four genes displayed size polymorphisms. By PCR amplification, the genes for the toxins staphylococcal enterotoxin A (SEA), SEC, SED, SEG, SEI, SEJ, and TSST-1 but not those for SEB, SEE, SEH, and the exfoliative toxins ETA and ETB could be detected. To analyze the epidemiological relationships, the isolates were subjected to DNA fingerprinting by macrorestriction analysis of their chromosomal DNAs. According to the observed gene polymorphisms, the toxin patterns, and the information given by macrorestriction analysis of the isolates by pulsedfield gel electrophoresis, a limited number of clones seemed to be responsible for the cases of bovine mastitis on the various farms.

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Typing of Staphylococcus aureus Isolated from Nasal Carriers

Cited by 15 publications

References 19 publications

The significance of nasal carriage of Staphylococcus aureus as risk factor for human skin infections

The significance of nasal carriage of Staphylococcus aureus as risk factor for human skin infections

Molecular Epidemiology of Microorganisms Isolated from Food Workers and Enteral Feeding of Public Hospitals

Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis

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