Typing of methicillin-resistant Staphylococcus aureus isolates from Dusseldorf by six genotypic methods

Schmitz, F.-J.; Steiert, Matthias; Tichy, Hans-Volker; Hofmann, B.; Verhoef, J.; Heinz, Hans‐Peter; Köhrer, Karl; Jones, Mark

doi:10.1099/00222615-47-4-341

Cited by 69 publications

(59 citation statements)

References 11 publications

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“…Previous studies have shown that hypervariable region analysis by PCR and sequencing can be used to distinguish different mec DNA types (191,233,308). Five to eighteen different HVR-types have been identified in collections of 24-254 strains on the basis of differences in either the number or the sequence of dru repeats (185,191,242,246,308). Consistently with the present study, the same HVRtype was often found in different genotypes.…”

Section: Mec Hypervariable Regionsupporting

confidence: 90%

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Finland

Salmenlinna¹,

Lyytikäinen

Kotilainen

et al. 2000

European Journal of Clinical Microbiology & Infectious Dise

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Section: Mec Hypervariable Regionsupporting

confidence: 90%

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Finland

Salmenlinna¹,

Lyytikäinen

Kotilainen

et al. 2000

European Journal of Clinical Microbiology & Infectious Dise

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“…The use of a sequencebased typing method, like MLST, facilitated rapid comparisons with other S. aureus strains previously typed using this method via the MLST web site (http://saureus.mlst.net). The clustering of isolates obtained by MLST, and the level of discrimination, is largely consistent with that obtained by PFGE [11,12,13].…”

Section: Resultssupporting

confidence: 65%

Ovine clone ST1464: A predominant genotype of Staphylococcus aureus subsp. anaerobius isolated from sheep in Sudan

Elbir

Feil

Drancourt

et al. 2010

J Infect Dev Ctries

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Background: The aim of the present study was to examine the phenotypic and genotypic relatedness of 17 Staphylococcus aureus subsp. anaerobius isolates recovered from sheep abscesses in Khartoum state, Sudan, during the period [2007][2008]. Methodology: This sample was characterised using antibiogram typing, biochemical typing with the commercial PhenePlate system (PhP-CS) and multilocus sequence typing (MLST). Results: Low levels of resistance were noted to the 11 antimicrobial agents tested. All the isolates corresponded to a single PhP type, and to a single, novel, multilocus sequence type, designated ST1464.Conclusion: These results demonstrate that the vast majority of cases of sheep abscess disease in Khartoum state are caused by a single novel clone of S. aureus subsp. anaerobius.

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“…Monitoring the hospital distribution of MRSA by application of DNA-based molecular typing methods has significantly increased the resolution of epidemiological analysis (21,22). Restriction fragment length polymorphism analysis of genomic DNA using pulsed-field gel electrophoresis has been considered to be a reliable DNA-based method for differentiating MRSA strains and has proved to be superior to most of all other molecular typing methods due to its high discriminatory power and reproducibility (23)(24)(25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Use of molecular epidemiology to monitor the nosocomial dissemination of methicillin-resistant Staphylococcus aureus in a University Hospital from 1991 to 2001

Beretta

Trabasso

Stucchi

et al. 2004

Braz J Med Biol Res

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Methicillin-resistant Staphylococcus aureus (MRSA) has been the cause of major outbreaks and epidemics among hospitalized patients, with high mortality and morbidity rates. We studied the genomic diversity of MRSA strains isolated from patients with nosocomial infection in a University Hospital from 1991 to 2001. The study consisted of two periods: period I, from 1991 to 1993 and period II from 1995 to 2001. DNA was typed by pulsed-field gel electrophoresis and the similarity among the MRSA strains was determined by cluster analysis. During period I, 73 strains presented five distinctive DNA profiles: A, B, C, D, and E. Profile A was the most frequent DNA pattern and was identified in 55 (75.3%) strains; three closely related and four possibly related profiles were also identified. During period II, 80 (68.8%) of 117 strains showed the same endemic profile A identified during period I, 18 (13.7%) closely related profiles and 18 (13.7%) possibly related profiles and, only one strain presented an unrelated profile. Cluster analysis showed a 96% coefficient of similarity between profile A from period I and profile A from period II, which were considered to be from the same clone. The molecular monitoring of MRSA strains permitted the determination of the clonal dissemination and the maintenance of a dominant endemic strain during a 10-year period and the presence of closely and possibly related patterns for endemic profile A. However, further studies are necessary to improve the understanding of the dissemination of the endemic profile in this hospital.

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Typing of methicillin-resistant Staphylococcus aureus isolates from Dusseldorf by six genotypic methods

Cited by 69 publications

References 11 publications

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Finland

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus in Finland

Ovine clone ST1464: A predominant genotype of Staphylococcus aureus subsp. anaerobius isolated from sheep in Sudan

Use of molecular epidemiology to monitor the nosocomial dissemination of methicillin-resistant Staphylococcus aureus in a University Hospital from 1991 to 2001

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