Typing of Foot and Mouth Disease Virus circulating in Bangladesh by Reverse Transcription Polymerase Chain Reaction

Abstract

Foot and Mouth Disease (FMD) is one of the highly contagious diseases of domestic and wild animals of many countries of the world including Bangladesh. The present study was undertaken to detect currently circulating FMD virus (FMDV) in Bangladesh using BHK-21 cell line, and the FMDV were typed using RT-PCR. The viruses induced characteristics cytopathic effects in BHK-21 cell lines, for examples, rounding, swelling, breaking down of intercellular bridge and cell death. Out of 151 clinical samples collected from different districts of Bangladesh, 85(56.29%) were successfully adapted in BHK-21 cell line, and were analyzed using FMDV type specific primers. Among these 85 BHK-21 adopted virus, 71(83.52%) were found as positive for FMD virus by RT-PCR; of which, 31 (36.47%) were positive for type A, 26 (30.58%) for type O, and 10 (11.76%) for type Asia 1. Only 4 (4.70%) samples were found as positive for mixed infection having Type A and Asia 1 FMDV. All the FMDV originated from Tangail (n=7) and Rajshahi (n=6) districts were found as positive for FMDV while 71.42% (n=14/36) samples of Mymensingh district were positive for FMDV. The FMDV identified in this study could now be used for effective vaccine development to control the disease in Bangladesh.

The clinicopathology of foot and mouth disease and molecular epidemiology of the viruses circulated in cattle of Pabna district, Bangladesh

“…4). Similar observation was also reported by Loth et al (2011) and Hossen et al (2014). However, effect of this mutation on the virulence of FMD virus was not evaluated.…”

RT-PCR based detection and adaptation of foot and mouth disease virus serotype “A” in BHK-21 cell line

“…Mixed infection having Type A and Asia-1 FMDV was also detected (Hossen et al, 2014). Epidemiological investigation of this virus in cattle population indicated that four different types (A, O, C and Asia-1) of FMDV were prevalent in Bangladesh during 1960 to 1990 (Chowdhury et al, 1996).…”

“…Detection of FMDV PCR has been described , 2015; Hossen et al, 2014;Loth et al, 2011;Saeed et al, 2011). We have performed PCR after synthesis of cDNA from the extracted RNA of the FMD virus and found positive bands at 866bp after electrophoresis in 2% agarose gel which was specific for FMDV serotype "A" (Figure 2 Present study proved that FMDV serotype "A" is present in the Pabna district of Bangladesh.…”

“…The recent studies indicated that three different types (A, Asia-1 and O) were prevalent in Bangladesh during 2007 to 2008 (Sil and Taimur, 2000;Zinnah et al, 2010;Nandi et al, 2013). Serotype "O" was found to be responsible for 80% of the confirmed outbreaks, whereas "Asia 1" and "A" caused 12% and 8% outbreaks, respectively (Sarker et al, 2011;Hossen et al, 2014).…”

Clinically detection of foot and mouth disease at Kapasia upazila under Gazipur dristrict in Bangladesh

“…144.0 /animal), draught energy wastage average 12.48 kilo watt hr/animal, and land left uncultivated average .72/ animal (Rahman et al, 1985). A lot of epidemiological and molecular research works have been conducted on FMD virus but in clinical aspects has few studied in Bangladesh (Islam et al, 2000;Howlader et al, 2004;Sarker et al, 2011;Hossen et al, 2014;Rahman et al, 2015) and in the world (Edwards, 2004;Durand et al, 2008;Fernandez et al, 2008;Ryan et al, 2008;Huang et al, 2011). There is no published data so far on prevalence of FMD in Gazipur district of Bangladesh although this region has huge number of livestock population.…”