Typing of Aeromonas hydrophila of fish and human diarrhoeal origin by outer membrane proteins and lipopolysaccharides

Subashkumar, R.; Vivekanandhan, G.; Raja, S.; Natarajaseenivasan, Kalimuthusamy; Thangavelu, Thayumanavan; Lakshmanaperumalsamy, P.

doi:10.1007/s12088-007-0009-7

Cited by 7 publications

(8 citation statements)

References 24 publications

(15 reference statements)

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“…The detection of A. hydrophila in muscle of fish intended for consumption is significant and grave as it could act as a potential source of resistant bacteria for humans. Similar studies all over the world have consistently documented multiple antibiotic resistance to A. hydrophila [50,36,37,51,34].This study also further corroborates the reports of excessive and uncontrolled use of antimicrobials in Nigerian aquaculture [27] and other livestock industries [28,29,30] leading to the development of antimicrobial resistant pathogens.…”

Section: Resultssupporting

confidence: 85%

“…The resistance to augmentin (100%) recorded in this study agrees with records of [36] who also recorded 100% resistance to Ticarcillin/clavulanic acid and 92.6% to Amoxillin/Subactam (AMX-SUL) by Trifamox IBL(Laboratories Bagó, Buenos Aires, Argentina). Nitrofurantoin demonstrated the highest susceptibility (70%) in all isolates tested, this is close to a previously report of 100% by [34] but contrary to that of [37] who reported 42% susceptibility to nitrofurantoin. Such differences may be clearly demonstrates the kinetic ability of bacterial organisms in developing resistance to antimicrobials.…”

Section: Discussionsupporting

confidence: 83%

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Multiple antibiotic resistance indices of Aeromonas hydrophila isolates of muscle of catfish (Clarias gariepinus, Burchell 1822) from selected markets in Ibadan, Nigeria

Saka¹,

Adeyemo²,

Odeseye³

2017

Af J Clin Exp Micro

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The extensive use and misuse of antimicrobials for treatment and prophylaxis in livestock production generally and aquaculture in particular is of great concern to environmental and public health. In Nigeria, regulation and monitoring of aquaculture and other livestock production activities at best is lax. Drug resistance pathogens have therefore been consistently reported in Nigeria. Ninety-eight adult live fishes weighing an average of 684.88±141.73g were purchased at random from different live-fish selling points fortnightly over a fourteen-week period. Fish were anaesthetized using Tricaine Methane Sulfonate (MS222 and 15g of muscle excised and processed according to standard methods. Growth, isolation and characterization of Aeromonas hydrophila was accomplished using Rimler-Shotts agar medium which had been infused with ampicillin supplement for 24 hours and incubated at 37oC and appropriate biochemical tests.Ten positive isolates (AH1-AH10) were subjected to culture and sensitivity test using the disc diffusion method on nutrient agar. Zones of growth inhibition around the colonies were observed, measured and characterized as sensitive, intermediate and resistant based on the Manual of Antimicrobial Susceptibility Testing method. All the isolates had MAR >0.2. Isolate AH9 had the highest MAR index (1). Three of the isolates (AH3, AH5 and AH8) had MAR indices of 0.89, while AH2, AH4 and AH7 had MAR indices of 0.67. This study established the resistance of Aeromonas hydrophila isolates from fish muscle to a wide range of antibiotic. The detection of high MAR A. hydrophila in muscle of fish intended for consumption is significant and could act as a potential source of resistant bacteria for humans. Further investigation into antimicrobial resistance is recommended. une source potentielle de bactéries résistantes pour les humains. Enquête plus approfondie sur des gènes résistants aux antimicrobiens provenant de cette source est recommandée par les présentes.

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Section: Resultssupporting

confidence: 85%

Section: Discussionsupporting

confidence: 83%

Multiple antibiotic resistance indices of Aeromonas hydrophila isolates of muscle of catfish (Clarias gariepinus, Burchell 1822) from selected markets in Ibadan, Nigeria

Saka¹,

Adeyemo²,

Odeseye³

2017

Af J Clin Exp Micro

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“…We think it is important for the readers of the journal to look at other interpretations and conclusions published on this subject worldwide. In this sense, the association of Aeromonas and diarrhoea symptoms in both children and adults has been described in studies from all over the world (Janda & Abbott, 1998;Chan & Ng, 2004;Figueras, 2005;Martin-Carnahan & Joseph, 2005;Subashkumar et al, 2006). The latest studies have reported that 2-20 % of the diarrhoeal cases from children around the world are induced solely by species of Aeromonas, and that only 0-2 % of children that harbour Aeromonas do not present with diarrhoeal symptoms (Galindo et al, 2006).…”

mentioning

confidence: 94%

Controversial data on the association of Aeromonas with diarrhoea in a recent Hong Kong study

Figueras

Horneman

Martínez‐Murcia

et al. 2007

Journal of Medical Microbiology

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“…Then, 50 or 100 µl of 1x PBS solution was added to the pellet and mixed well. This extracellular protein was stored at -20°C for further use (modified protocol of Subashkumar et al, 2007). Extracellular protein separation was made by 12.5% of SDS-gel SDS-PAGE electrophoresis (Lamelli, 1970).…”

Section: Extracellular Protein Profilementioning

confidence: 99%

Genetic analysis of antibiotic production and other phenotypic traits from Streptomyces associated with seaweeds

Sridevi¹,

Dhevendaran²

2014

Afr. J. Biotechnol.

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The Gram-positive bacterium such as streptomycetes known for its production of a diverse array of biotechnologically important secondary metabolites, have major application in health, nutrition and economics of our society. There are limited studies on the genetics of streptomycetes, especially seaweed associated Streptomyces sp. So, the present study made an attempt to study the genetics of production of antibiotic and other phenotypic properties was demonstrated by plasmid DNA curing analysis. The DNA-intercalating agent ethidium bromide was used to eliminate plasmid DNA from streptomycetes and effects of curing agent (EB) on the antibiotic production and loss of other phenotypic traits such as aerial and substrate mycelial production, biomass production, protein synthesis were studied. The study demonstrates that the ethidium bromide is potent and probably region-specific mutagens that are capable of inducing high rates of plasmid loss (curing), production of antibiotics was not eliminated, but was reduced by 20.2-79.8% and extracellular protein of 26 KDa mol.wt. was unaffected by curing agents. Data suggests that production of antibiotics and other phenotypic traits likely chromosomally encoded in marine Streptomyces species. The study concludes that the new methodologies such as mutasynthesis have contributed substantially to the discovery of additional antibiotics as an added feather to the scope of antibiotic industry.

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Typing of Aeromonas hydrophila of fish and human diarrhoeal origin by outer membrane proteins and lipopolysaccharides

Cited by 7 publications

References 24 publications

Multiple antibiotic resistance indices of <i>Aeromonas hydrophila</i> isolates of muscle of catfish (<i>Clarias gariepinus</i>, Burchell 1822) from selected markets in Ibadan, Nigeria

Multiple antibiotic resistance indices of <i>Aeromonas hydrophila</i> isolates of muscle of catfish (<i>Clarias gariepinus</i>, Burchell 1822) from selected markets in Ibadan, Nigeria

Controversial data on the association of Aeromonas with diarrhoea in a recent Hong Kong study

Genetic analysis of antibiotic production and other phenotypic traits from Streptomyces associated with seaweeds

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