Typing for MLC Determinants using Lymphocytes from Cousin‐Marriage Offspring as Typing Cells

Singal, D. P.; Villanueva, Ronald; Naipaul, N.

doi:10.1111/j.1399-0039.1975.tb00604.x

Cited by 4 publications

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“…HLA-D typing was done by the micromixed lymphocyte culture method previously described (Singal et al 1975). The homozygous typing cells utilized in HLA-D typing were obtained from Drs.…”

Section: Methodsmentioning

confidence: 99%

“…The strongest proliferation is caused by disparity at the HLA-D locus, closely linked to but distinct from the HLA-B locus (Yunis & Amos 1971). The typing for these HLA-D determinants is done with the use of stimulating homozygous typing cells (HTC) obtained from HLA-D homozygous individuals (J$rgensen et al 1973, Singal et al 1975, Mempel et al 1973, van den Tweel et al 1973). This HTC method has been used to recognize the polymorphism of the HLA-D-stimulating products.…”

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confidence: 99%

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The Specificity of Secondary MLC Assayed by Titration of Primed Lymphocyte Populations

Singal

1979

Tissue Antigens

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The specificity of responses in secondary MLC was studied by titration (100 × 103 to 5 × 103) of responder primed lymphocytes. In all instances, significant proliferative responses of responder primed lymphocytes to the specific stimulator occurred using lower responding cell numbers. When tested against allogeneic stimulating donors, three patterns of responses were observed: no significant responses, responses only at higher (100 × 103) responding cell densities, and responses at lower responding cell densities, similar to those with the priming donor. In instances where primed lymphocytes responded significantly to allogeneic stimulating cells at lower cell densities, the responses were considered positive and the stimulating cells positive for the PL determinant. In several instances, primed lymphocytes responded significantly to allogeneic stimulators negative for the specific HLA—D and/or HLA—DR specificities. On the other hand, in experiments where allogeneic stimulating donors shared either HLA—D or DR specificity with the priming donor, a significant response was always observed. Thus, the PL determinant was present on stimulating allogeneic cells negative for specific HLA—D and DR specificities. The present data suggest that an analysis of the specificity of primed populations could be profoundly affected by the responder cell density at which the assay is performed. Also, the data suggest that other MHC determinants, including non‐HLA loci may be important in secondary MLC.

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“…HLA-D typing was done by the micromixed lymphocyte culture method previously described (Singal et al 1975). The homozygous typing cells utilized in HLA-D typing were obtained from Drs.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%