Types of Myosin Light Chains Present during the Development of Fast Skeletal Muscle in Chick Embryo1

Abstract

The types of myosin light chains in developing fast skeletal muscle of chick embryo were characterized in terms of their mobilities on SDS-polyacrylamide gel electrophoresis and their reactions with antisera against adult fast, slow, or cardiac myosin light chains. Breast and leg muscles of adult chicken mainly contain fast-type myosin, while at early developmental stages, these muscles contain four classes of light chain as judged by SDS-polyacrylamide gel electrophoresis and immunoelectrophoresis; two of these light chains are of the slow and/or cardiac type and the other two are of the fast type. At late developmental stages, fast-type light chains are present in large amounts and slow and/or cardiac light chains exist only in small amounts. It is suggested that the qualitative changes in light chains during embryonic development are coordinated with those in heavy chains reported by Masaki and Yoshizaki (5).

Molecular cloning of the duck MyoG and MRF4 genes coding region sequence and their differential expression patterns in the breast and leg muscle during fetal development

“…In poultry, the developing breast muscle is mainly composed of fast muscles, whereas the developing leg muscles in the embryo are mainly composed of slow fibers (Obinata et al 1980). In the present study with ducks, both genes tended to have a trend that increases in expression at first and decreases later, similar to observations made in the chicken (Yamane et al 2000).…”

“…These data mainly concentrated on mammals of different breeds. In poultry, the developing embryonic breast muscles (BM) are mainly composed of fast muscle, whereas the developing leg muscles (LM) are mainly composed of slow muscle (Obinata et al 1980). So far, little is known about whether MyoG and MRF4 have different expression patterns in poultry BM and LM during embryonic and fetal development, and needs further elucidation.…”

Transition from Non-Muscle to Muscle Myosin Light Chains in Chick Embryo Development

“…2, lane e), there are six light chains, three of which comigrate with the adult light chains of the fast type (16,000, 18,000 and 25,000) and two comigrate with the slow type 20,000 and 27,000. This pat tern is not an unusual one in embryonic muscle, in which, as many authors have shown [12,13], there are both the fast and slow light chains, and only at the time of birth does the type of innervation determine the terminal differentiation into fast and slow fibers [14], It is interesting that, in our extracts of 13-and 42-stage embryos, traces of another light chain were present, a 15,000 chain. The results reported here suggest that the appearance of myosin light chain is developmentally regulated, changing from a non-muscle to a muscle type during the dif ferentiation of the myotome.…”

Régulation de l'expression des gènes α et β de la tropomyosine au cours du développement du muscle pectoral chez le poulet

“…Les protéines contractiles identifiées dans la totalité de la masse du muscle pectoral embryonnaire sont les isoformes a et fi rapides de la tropomyosine (Roy et al, 1979 ; Matsuda et a/., 1983) et une faible quantité de tropomyosine lente (Matsuda et al, 1983), les isoformes LC1 et LC2 rapides (les formes lentes sont très minoritaires) des chaînes légères de la myosine (Hoh, 1979 ;Obinata et al, 1980b ;Matsuda et al, 1983), une isoforme embryonnaire de la chaîne lourde de la myosine rapide (Bandman et (Hoh, 1979) (Rosenberg et al, 1985) de l'isoforme musculaire de la créatine kinase (M-CK) seule présente dans le muscle après différenciation (Lough et Bischoff, 1977). Dans le muscle pectoral, le messager {3 est accumulé en plateau ( fig.…”