Type-specific human papillomavirus-DNA load in anal infection in HIV-positive men

Pierangeli, Alessandra; Scagnolari, Carolina; Degener, Anna Marta; Bucci, Mauro; Ciardi, Antonio; Riva, Elisabetta; Indinnimeo, M; Mancini, Giuseppe; d’Ettorre, Gabriella; Vullo, Vincenzo; Antonelli, Guido

doi:10.1097/qad.0b013e32830fbd7a

Cited by 31 publications

(32 citation statements)

References 34 publications

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“…An obvious feature of this method is that, while adding information on the specific HPV genotype, testing all mucosal HPVs in a sample is not routinely feasible. In this study we detected the HR HPV genotypes associated with the higher percentage of cervical cancer cases (16,18,31,33) and the other HPV genotypes (the HR HPVs 53 and 58, and the LR HPVs 6 and 11) more frequently detected in Rome according to our previous paper [17]. Testing a limited number of genotypes can also explain the fact that no multiple infection were detected in this study.…”

Section: A C C E P T E D Accepted Manuscriptsupporting

confidence: 62%

“…To overcome this problem, we used HPV type-specific quantitative real-time PCR (qPCR) fluorogenic assays, implemented in a previous study [18], that provide a greater analytical sensitivity without requiring intact genomic DNA. To avoid false negative results, all samples were tested for the quality of…”

Section: Hpv Detectionmentioning

confidence: 99%

“…Accordingly, HPV type-specific primers and TaqMan probes for the more common low-risk (HPV 6, 11) and high-risk (16,18,31,33,53, 58) HPV genotypes were used and copy numbers measured in samples by means of HPV plasmid external curves [18]. Total DNA concentration was calculated by OD 260 reading and viral load was determined as HPV copy number per nanogram of total DNA in each sample [18].…”

Section: Hpv Detectionmentioning

confidence: 99%

“…Human papillomavirus (HPV) genotype and viral loads in oral samples from case and control groups 16[18]. 18[6],31[1],33[2],53[1],58[1] HR HPV viral load, median (range) e LR HPV viral load, median (range) e 2x10 4 (4.3x10 2 -1.3x10 7 )2.8 x10 3 (4.3x10 1 -4.4 x10 3 )…”

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confidence: 99%

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Frequent detection of high human papillomavirus DNA loads in oral potentially malignant disorders

Pierangeli

Cannella

Scagnolari

et al. 2016

Clinical Microbiology and Infection

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Human papillomavirus (HPV) is estimated to be the cause of 40--80% of the squamous cell carcinoma of the oropharynx but only of a small fraction of the oral cavity cancers. The prevalence of oral HPV infection has significantly increased in the last decade, raising concerns about the role of HPV in progression of oral potentially malignant disorders (OPMD) toward squamous cell carcinomas. We sought to study HPV infection in patients with oral lesions, and in control individuals, using non-invasive and site-specific oral brushing and sensitive molecular methods. HPV DNA positivity and viral loads were evaluated in relation to patient data and clinical diagnosis. We enrolled 116 individuals attending Dental Clinics: 62 patients with benign oral lesions (e.g. fibromas, papillomatosis, ulcers) or OPMD (e.g. lichen, leukoplakia) and 54 controls. Oral cells were collected with Cytobrush and HPV-DNA was detected with quantitative real-time PCR for the more common high-risk (HR) and low-risk (LR) genotypes. HPV detection rate, percentage of HR HPVs and HPV-DNA loads (namely HPV16 and in particular, HPV18) were significantly higher in patients than in controls. Lichen planus cases had the highest HPV-positive rate (75.0%), hairy leukoplakia the lowest (33.3%). This study detected unexpectedly high rates of HPV infection in cells of the oral mucosa. The elevated HR HPV loads found in OPMD suggest the effectiveness of quantitative PCR in testing oral lesions. Prospective studies are needed to establish whether elevated viral loads represent a clinically useful marker of the risk of malignant progression.

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Section: A C C E P T E D Accepted Manuscriptsupporting

confidence: 62%

Section: Hpv Detectionmentioning

confidence: 99%

Section: Hpv Detectionmentioning

confidence: 99%

mentioning

confidence: 99%

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Frequent detection of high human papillomavirus DNA loads in oral potentially malignant disorders

Pierangeli

Cannella

Scagnolari

et al. 2016

Clinical Microbiology and Infection

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“…Notably, the overall HPV prevalence found was higher than that observed in other cohorts of HIV-negative MSM, 11,12 although lower than that reported by most studies on the HIV-positive counterpart. 5,11,13 The high sensitivity and number of HPV types detected by the method utilized in this investigation may explain the elevated HPV prevalence, the high rate of multiple infection and mean number of types among HPV-positive patients. With respect to other sexually active MSM, 12 a higher rate of infection by HR-HPV was observed in our cohort.…”

Section: Discussionmentioning

confidence: 91%

Prevalence, genotype diversity and determinants of anal HPV infection in HIV-uninfected men having sex with men

Donà

Palamara

Carlo

et al. 2012

Journal of Clinical Virology

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HPV status and HPV16 viral load in anal cancer and its association with clinical outcome

et al. 2022

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Background:The incidence of anal cancer is increasing globally. Evidence-based improvement in early detection and management of this morbid cancer is thus required. In other cancers associated with Human Papillomavirus (HPV), viral status and dynamics, including viral load (VL) has been shown to influence clinical outcome. Our aim was to determine the influence of HPV status and HPV16 VL on the clinical outcomes of anal cancer patients.Methods: A total of 185 anal cancer lesions were genotyped for HPV. Of the HPV16 positive component, VL was determined using a digital droplet PCR assay.The association of qualitative HPV status and VL (low (<12.3), medium (12.3-57) and high (>57 copies/cell)) on overall survival and hazard of death was assessed.Results: Of the 185 cases, 164 (88.6%) samples were HPV positive. HPV16 was detected in 154/185 samples (83.2%). HPV positive status was associated with improved overall survival in the univariate analysis [hazard ratio (HR) of 0.44, 0.23-0.82, p = 0.01]. When adjusted by age, sex, stage and response to treatment, the association of positive HPV status with improved survival remained (HR 0.24 [0.11-0.55] p < 0.001). High VL was associated with improved overall survival in the univariate analysis with a HR of 0.28 (0.11-0.71, p = 0.007). When adjusted only by age and sex, high VL was associated with better overall survival (HR 0.27, 0.11-0.68 p = 0.006).Conclusions: HPV status appears to be independently associated with improved outcomes in anal cancer patients. Moreover, HPV viral load quantification may be informative for further risk stratification and warrants further investigation.

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Type-specific human papillomavirus-DNA load in anal infection in HIV-positive men

Abstract: Type-specific HPV-DNA copies at baseline appear to be independent of patient immune status and of HPV genotype. HPV genotype risk and viral load should be further evaluated for their potential predictive role in persistence and progression.

Cited by 31 publications

References 34 publications

Frequent detection of high human papillomavirus DNA loads in oral potentially malignant disorders

Frequent detection of high human papillomavirus DNA loads in oral potentially malignant disorders

Prevalence, genotype diversity and determinants of anal HPV infection in HIV-uninfected men having sex with men

HPV status and HPV16 viral load in anal cancer and its association with clinical outcome

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