Type-specific action of vibriocidal antibody onVibrio cholerae

Abstract

SUMMARYThe vibriocidal activity of Inaba and Ogawa antisera, from which cross-reacting agglutinin had been absorbed, was specific for Vibrio cholerae strains of the homologous serotype. No vibriocidal action against strains of the heterologous type was detected.The sera appeared to be equally effective in killing organisms of different biotypes (classical, intermediate, and ElTor), provided that these were of the homologous serotype (Inaba or Ogawa). However, they had been raised against strains of the classical biotype only; and sera resulting from immunization with other biotypes had not been prepared.The implications of these findings in immunity to cholera are discussed.

Corresponding type-specificity of vibriocidal and agglutinating activities ofVibrio choleraeantisera: relevance to vaccine immunogenicity

“…See footnotes to Table 1. available sera in an attempt to re-confirm the existence of serotype specificity in vibriocidal activity [6]. In the first such experiment ( heterologous bacteria, however, the agglutination results formed a pattern very similar to that obtained with the vibriocidal test (above), as described below.…”

“…The tests were performed in plastic microtitration plates with U cups, as described in detail previously [6].…”

“…Regardless of biotype, strains of cholera vibrio can be typed with antiserum into one of two serotypes, Ogawa and Inaba; and such sera have been shown to produce serotype-specific killing of the bacteria in a vibriocidal test [6]. It has been known for many years that vibriocidal titres of human sera correlate with immunity in family contacts of cholera patients [7], and that such immunity is 17 HYG 11( serotype-specific [8].…”

Length of the linker and the interval between immunizations influences the efficacy ofVibrio choleraeO1, Ogawa hexasaccharide neoglycoconjugates

“…The upstream terminal sugar of the O‐SP of V. cholerae LPS differentiates the Ogawa and Inaba serotypes. A 2‐ O ‐methyl group (Pykett & Preston, 1975; Hisatsune et al , 1993; Ito et al , 1994; Villeneuve et al , 2002) defines Ogawa LPS, while the Inaba terminal sugar has a hydroxyl element in that position (Hisatsune et al , 1993; Liao et al , 2002). Protective Ogawa specific monoclonal antibodies (mAbs) bind the O‐SP upstream terminal sugar (Liao et al , 2002; Villeneuve et al , 2002), while other structures in common (core‐O‐SP junction) between Ogawa and Inaba also can induce protective antibodies (Villeneuve et al , 1999).…”

Immunogenicity of synthetic saccharide fragments ofVibrio choleraeO1 (Ogawa and Inaba) bound to Exotoxin A

“…The upstream terminal sugar of the O-SP of V. cholerae LPS differentiates the Ogawa and Inaba serotypes. A 2-O-methyl group (Pykett & Preston, 1975;Ito et al, 1994;Liao et al, 2002;Villeneuve et al, 2002) defines Ogawa LPS (sero-epitope B) while the Inaba terminal sugar with a hydroxyl group at that position is thought to define sero-epitope C (Manning et al, 1994;Liao et al, 2002;Villeneuve et al, 2002). Protective Ogawaspecific mAbs bind the O-SP upstream terminal sugar (Wang et al, 1998;Chernyak et al, 2002).…”